Date published: 2025-9-6
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Nectin 3 Antibody (H-11) - Western Blotting - Image 356945
Nectin 3 抗体 (H-11): sc-271611. NTERA-2 cl. D1 (A), JAR (B), F9 (C), C3H/10T1/2 (D), NRK (E) 和 RIN-m5F (F) 全细胞裂解液中 Nectin 3 表达的 Western 印迹分析.
Nectin 3 抗体 (H-11): sc-271611. NTERA-2 cl. D1 (A), JAR (B), F9 (C), C3H/10T1/2 (D), NRK (E) 和 RIN-m5F (F) 全细胞裂解液中 Nectin 3 表达的 Western 印迹分析.

Nectin 3 抗体 (H-11): sc-271611

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说明书
  • Nectin 3 抗体 H-11 是小鼠单克隆 IgG1 κ，Nectin 3抗体, 在1篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列301-545位于human物种的Nectin 3的C-terminus附近
  • Nectin 3 抗体 (H-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Nectin 3
  • 抗Nectin 3抗体（H-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nectin 3 (H-11): sc-271611的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Nectin 3 Antibody (H-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Nectin 3 Antibody (H-11) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Nectin抗体，包括Nectin 1, Nectin 2, Nectin 3和Nectin 4

Nectin 3抗体（H-11）是一种IgG1 κ小鼠单克隆Nectin 3抗体（也称为NECTIN3抗体或PVRL3抗体），它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Nectin 3蛋白。Nectin 3抗体（H-11）既可以以非偶联抗Nectin 3抗体的形式存在，也可以以多种偶联形式的抗Nectin 3抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Nectin是一种不依赖于Ca2+的同型细胞粘附分子，属于免疫球蛋白超家族。人类Nectin与脊髓灰质炎病毒受体相关蛋白（PRR）相同，被确定为α疱疹病毒进入介体。Nectin构成一个家族，至少包括Nectin 1、2和3。Nectin 2和3在体内普遍表达，而Nectin 1在大脑中大量表达。Nectin 3，也被称为PRR3，有三种剪接变体：Nectin 3α、3β和3γ。Nectin 3是一种跨膜蛋白，其胞外区含有三个类似Ig的结构域。Nectin 3α和3β，而不是Nectin 3γ，具有一个C端保守基序（E/A-X-Y-V）。该基序与F-Actin结合蛋白afadin的PDZ结构域相互作用，通过该结构域与Actin细胞骨架相连。Nectin 3α与Nectin 2在基于钙粘蛋白的粘附连接处共定位，并与afadin相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nectin 3 抗体 (H-11) 参考文献:

  1. 在上皮细胞极化连接排列的形成和破坏过程中，nectin-afadin-ponsin 系统和 cadherin-catenin 系统之间存在相似和不同的行为。  |  Asakura, T., et al. 1999. Genes Cells. 4: 573-81. PMID: 10583506
  2. 花蜜素-3，免疫球蛋白样细胞黏合剂分子的新成员，具有同嗜性和异嗜性细胞-细胞黏合剂活性。  |  Satoh-Horikawa, K., et al. 2000. J Biol Chem. 275: 10291-9. PMID: 10744716
  3. 细胞-细胞粘合剂介导的酪氨酸磷酸化 nectin-2delta，一种免疫球蛋白样细胞粘合分子，位于粘合剂连接处。  |  Kikyo, M., et al. 2000. Oncogene. 19: 4022-8. PMID: 10962558
  4. 人类 nectin3/PRR3：与 afadin 相互作用的 PVR/PRR/nectin 家族新成员。  |  Reymond, N., et al. 2000. Gene. 255: 347-55. PMID: 11024295
  5. 坐骨神经切断后脊髓运动神经元中nectin-1、nectin-3、N-cadherin和NCAM的表达。  |  Zelano, J., et al. 2006. Exp Neurol. 201: 461-9. PMID: 16777094
  6. Nectin-3 (CD113) 与 Nectin-2 (CD112) 相互作用，促进淋巴细胞跨内皮迁移。  |  Devilard, E., et al. 2013. PLoS One. 8: e77424. PMID: 24116228
  7. 着床时子宫上皮细胞连接处的 Nectin-3 增加了  |  Poon, CE., et al. 2016. Reprod Sci. 23: 1580-1592. PMID: 27217376
  8. 花蜜素-3能调节齿状颗粒细胞的结构可塑性和长期记忆。  |  Wang, XX., et al. 2017. Transl Psychiatry. 7: e1228. PMID: 28872640
  9. Nectin-3 是一种新的生物标记物，它能介导卵巢癌细胞中 MMP2 和 MMP9 的上调。  |  Xu, F., et al. 2019. Biomed Pharmacother. 110: 139-144. PMID: 30469078
  10. 胰腺神经内分泌肿瘤中作为肿瘤侵袭性标志物的 nectin-3 表达缺失。  |  Hirabayashi, K., et al. 2020. Pathol Int. 70: 84-91. PMID: 31855317
  11. 有证据表明大鼠角膜内皮细胞表面的大豆凝集素结合蛋白是花蜜素-3。  |  Gordon, SR., et al. 2022. Exp Eye Res. 223: 109212. PMID: 35964705
  12. 与脊髓灰质炎病毒受体编码基因有关的人类基因的互补 DNA 特征和染色体定域。  |  Lopez, M., et al. 1995. Gene. 155: 261-5. PMID: 7721102

订购信息

产品名称产品编号规格价格数量收藏夹

Nectin 3 抗体 (H-11)

sc-271611
200 µg/ml
$316.00

Nectin 3 (H-11): m-IgG Fc BP-HRP 套装

sc-529222
200 µg Ab; 10 µg BP
$354.00

Nectin 3 (H-11): m-IgGκ BP-HRP 套装

sc-521997
200 µg Ab, 40 µg BP
$354.00

Nectin 3 (H-11): m-IgG1 BP-HRP 套装

sc-543397
200 µg Ab; 20 µg BP
$354.00

Nectin 3 抗体 (H-11) AC

sc-271611 AC
500 µg/ml, 25% agarose
$416.00

Nectin 3 抗体 (H-11) HRP

sc-271611 HRP
200 µg/ml
$316.00

Nectin 3 抗体 (H-11) FITC

sc-271611 FITC
200 µg/ml
$330.00

Nectin 3 抗体 (H-11) PE

sc-271611 PE
200 µg/ml
$343.00

Nectin 3 抗体 (H-11) Alexa Fluor® 488

sc-271611 AF488
200 µg/ml
$357.00

Nectin 3 抗体 (H-11) Alexa Fluor® 546

sc-271611 AF546
200 µg/ml
$357.00

Nectin 3 抗体 (H-11) Alexa Fluor® 594

sc-271611 AF594
200 µg/ml
$357.00

Nectin 3 抗体 (H-11) Alexa Fluor® 647

sc-271611 AF647
200 µg/ml
$357.00

Nectin 3 抗体 (H-11) Alexa Fluor® 680

sc-271611 AF680
200 µg/ml
$357.00

Nectin 3 抗体 (H-11) Alexa Fluor® 790

sc-271611 AF790
200 µg/ml
$357.00

Are there any issues with using sc-271611: Nectin 3 (H-11) mouse monoclonal primary antibody on mouse samples?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271611 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Great antibodyWorks very well in human neural stem cells. Visible band at 100 kDa.
Date published: 2017-12-14
Rated 5 out of 5 by from Nectin-3 antibodyExcelent antibody for western blot. Detected Nectin-3 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti Nectin-3 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 5 out of 5 by from very satisfiedWB investigate the expression of Nectin 3 in breast cancer tissues ,very satisfied with strong signal at 83kda,use the dilution 1:800.
Date published: 2017-09-03
Rated 4 out of 5 by from good quality antibodyNectin 3 antibody is a good antibody for detection of Nectin 3 by WB (1:800, 5% BSA,TBST),we got specific band, but the IF result have some background signal,the condition should be optimized.
Date published: 2017-07-29
Rated 5 out of 5 by from gave strong signalThis antibody gave strong signal in Hepg2 cell lysates ,I got target band. Dilution was 1:1000 and there were no visibility of background signals.
Date published: 2017-07-08
Rated 5 out of 5 by from Produced nice Western blot data of NectinProduced nice Western blot data of Nectin 3 expression in 3T3-L1 whole cell lysate. -SCBT QC
Date published: 2014-06-26
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Nectin 3 抗体 (H-11) is rated 4.8 out of 5 by 6.
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