Date published: 2025-9-7

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NDUFS1 抗体 (E-8): sc-271510

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说明书
  • NDUFS1 抗体 E-8 是小鼠单克隆 IgG2b κ,NDUFS1抗体, 在20篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列428-727位于human物种的NDUFS1的C-terminus
  • NDUFS1 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 NDUFS1
  • 抗NDUFS1抗体(E-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NDUFS1 (E-8): sc-271510免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 NDUFS1 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NDUFS1 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NDUFS1抗体(E-8)是一种IgG2b κ小鼠单克隆NDUFS1抗体(也称为NDUFS1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的NDUFS1蛋白。NDUFS1抗体(E-8)既可以是非偶联的抗NDUFS1抗体形式,也可以是多种偶联形式的抗NDUFS1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。多亚基NADH:泛醌氧化还原酶(复合体I)是线粒体电子传递链中的第一个酶复合体。通过使用离液剂,复合体I可以被分离成3个不同的组分:一个黄素蛋白组分、一个铁硫蛋白(IP)组分和一个疏水蛋白(HP)组分。IP组分包含NDUFS1-7。NDUFS1是一种75kD的蛋白质,是复合体I中最大的亚基,被认为是复合体内第一个从NADH-黄素蛋白还原酶接受电子的铁硫蛋白。NDUFS1甚至可能是NADH被氧化的活性位点裂隙的一部分。NDUFS1也是线粒体中的关键半胱天冬酶底物,半胱天冬酶对NDUFS1的裂解是与凋亡相关的几种线粒体变化所必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NDUFS1 抗体 (E-8) 参考文献:

  1. 线粒体复合体 I 缺乏症中核 NDUFV1 和 NDUFS1 基因的大规模缺失和点突变。  |  Bénit, P., et al. 2001. Am J Hum Genet. 68: 1344-52. PMID: 11349233
  2. 人类 75-kDal Fe-S 蛋白的 NADH 辅酶 Q 还原酶基因(NDUFS1)在 2q33----q34 的定域。  |  Duncan, AM., et al. 1992. Cytogenet Cell Genet. 60: 212-3. PMID: 1505218
  3. 因 NDUFS1 基因发生新型突变而导致线粒体复合体 I 缺乏症的缔合作用莱氏综合征。  |  Martín, MA., et al. 2005. Arch Neurol. 62: 659-61. PMID: 15824269
  4. 高脂肪饮食会协调下调骨骼肌线粒体氧化磷酸化所需的基因。  |  Sparks, LM., et al. 2005. Diabetes. 54: 1926-33. PMID: 15983191
  5. 超声评估腹膜导管隧道在 CAPD 中与导管相关的感染。  |  Karahan, OI., et al. 2005. Int Urol Nephrol. 37: 363-6. PMID: 16142572
  6. 线粒体 NADH:泛醌氧化还原酶缺乏的细胞生物学后果。  |  Smeitink, JA., et al. 2004. Curr Neurovasc Res. 1: 29-40. PMID: 16181064
  7. 铁硫簇 N2 在 NADH:泛醌氧化还原酶(复合物 I)质子化反应中的可能作用。  |  Flemming, D., et al. 2005. J Mol Microbiol Biotechnol. 10: 208-22. PMID: 16645316
  8. 线粒体 NADH:泛醌氧化还原酶(复合体 I)中活性-非活性过渡敏感 SH 基团的拓扑结构和化学反应性。  |  Gostimskaya, IS., et al. 2006. Biochim Biophys Acta. 1757: 1155-61. PMID: 16777054
  9. 确定人类线粒体 NADH 辅酶 Q 还原酶 75-kDa Fe-S 蛋白的 cDNA 序列。  |  Chow, W., et al. 1991. Eur J Biochem. 201: 547-50. PMID: 1935949
  10. MiR-3130-5p是2q33的中间调节因子,通过靶向NDUFS1影响肺腺癌的侵袭性。  |  Zhan, J., et al. 2021. Cancer Med. 10: 3700-3714. PMID: 33978320
  11. 蛋白质组学分析确定NDUFS1和ATP5O作为前列腺癌生存结果的新标志物。  |  Wiebringhaus, R., et al. 2021. Cancers (Basel). 13: PMID: 34885151
  12. 有利于复合体I亚基NDUFS1谷胱甘肽化的条件在泛醌相关底物、甘油-3-磷酸和脯氨酸氧化后增加ROS的产生。  |  Wang, K., et al. 2022. Antioxidants (Basel). 11: PMID: 36290766

订购信息

产品名称产品编号规格价格数量收藏夹

NDUFS1 抗体 (E-8)

sc-271510
200 µg/ml
$316.00

NDUFS1 (E-8): m-IgG Fc BP-HRP 套装

sc-529197
200 µg Ab; 10 µg BP
$354.00

NDUFS1 (E-8): m-IgGκ BP-HRP 套装

sc-521964
200 µg Ab, 40 µg BP
$354.00

NDUFS1 抗体 (E-8) AC

sc-271510 AC
500 µg/ml, 25% agarose
$416.00

NDUFS1 抗体 (E-8) HRP

sc-271510 HRP
200 µg/ml
$316.00

NDUFS1 抗体 (E-8) FITC

sc-271510 FITC
200 µg/ml
$330.00

NDUFS1 抗体 (E-8) PE

sc-271510 PE
200 µg/ml
$343.00

NDUFS1 抗体 (E-8) Alexa Fluor® 488

sc-271510 AF488
200 µg/ml
$357.00

NDUFS1 抗体 (E-8) Alexa Fluor® 546

sc-271510 AF546
200 µg/ml
$357.00

NDUFS1 抗体 (E-8) Alexa Fluor® 594

sc-271510 AF594
200 µg/ml
$357.00

NDUFS1 抗体 (E-8) Alexa Fluor® 647

sc-271510 AF647
200 µg/ml
$357.00

NDUFS1 抗体 (E-8) Alexa Fluor® 680

sc-271510 AF680
200 µg/ml
$357.00

NDUFS1 抗体 (E-8) Alexa Fluor® 790

sc-271510 AF790
200 µg/ml
$357.00

I am using NDUFS1 (E-8): sc-271510 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody!Used it in Western Blot, strong and clean signal. Works better on nitrocellulose membrane than PVDF.
Date published: 2017-09-28
Rated 5 out of 5 by from Good antibodyI got the sample of NDUFS1 antibody (E-8) about 2 months ago. The antibody detected the band whose size is supposed to be NDUFS1 in 293T cells, and was very clean. When I transfected 293T cells with empty vector or NDUFS1 expression vector, WB with this antibody clearly showed that cells transfected with NDUFS1 expression vector had much higher levels than the cells transfected with empty vector.
Date published: 2017-05-02
Rated 5 out of 5 by from excellent for WBstrong, clear, low backgroud band was visualised in human cell lysate.
Date published: 2017-04-12
Rated 5 out of 5 by from Worked well in WBI used this antibody in WB (dilution 1:1000), the band is wonderful. No issue at all.
Date published: 2016-12-09
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-05-06
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NDUFS1 抗体 (E-8) is rated 5.0 out of 5 by 5.
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