Nanog 抗体 (5A10): sc-134218
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- Nanog 抗体 5A10 是小鼠单克隆 IgG2a, 在23篇文献中引用，提供 50 µg/500 µl
- raised against amino acids 1-154 corresponding to recombinant Nanog of human origin
- 推荐用于 mouse, rat 和 human 来源的Nanog WB, IP 和 ELISA检测
- 看 Nanog (1E6C4): sc-293121，可查看有标记的 Nanog 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
- 关于如何获取Nanog (5A10): sc-134218的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
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- Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
Is this Nanog (5A10): sc-134218 monoclonal antibody available directly conjugated to HRP?
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected]
Alternatively, consider using Nanog (1E6C4): sc-293121, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/nanog-antibody-1e6c4)
Date published: 2017-01-30
Rated 4 out of
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lucia66 from
Buon funzionamento
Ho provato l' anticorpo primario Nanog (5A10):sc-134218. Lo ho usato per western blot e devo dire che il risultato è stato soddisfacente. Ho usato una concentrazione (1:2000) più bassa di quella suggerita (1:1000) ed ho avuto un buon risultato lo stesso su due membrane su cui avevo corso 30µ e 40 µg di proteine. L’incubazione con il primario è stata fatta ON a 4°C ed ho usato un secondario anti mouse 1:20000. Le bande sono nette e c’è poco aspecifico. Dopo l’incubazione con primario e dopo l’incubazione con secondario ho eseguito 3x10 min di lavaggi con TPBS (PBS+0.1% Tween) ed il blocking è stato fatto per 4 ore con Milk al 5% in TPBS a temperatura ambiente.
Ritengo sia un prodotto valido per il mio tipo di ricerca e per il western blot in generale.
Date published: 2020-07-21
Rated 5 out of
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mulei from
very pleased
That was a very good antibody, diluted 1:1000 in human ovary tissue , we obtained good results.
Date published: 2017-05-11
Rated 5 out of
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sai_cheng from
great
Clean bands obtained in western blot using human ovary tissue extract.
Date published: 2017-04-17
Rated 5 out of
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Germaine from
Produced publishable WB data on human and mouse
Well characterized with publications in the primary literature for WB in human and mouse samples
Date published: 2017-01-11
Rated 5 out of
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ET from
WB: strong
WB: strong, clean band in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 5 out of
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SS from
Positive results in WB with transfected lysates
Positive results in WB with transfected lysates. -SCBT QC
Date published: 2015-02-03
Rated 5 out of
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LR from
Clean bands in western blot analysis of Nanog
Clean bands in western blot analysis of Nanog expression in HeLa and Raji whole cell lysates and human ovary tissue extract. -SCBT QC
Date published: 2013-08-25