Date published: 2025-9-6

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Nanog 抗体 (1E6C4): sc-293121

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说明书
  • Nanog抗体(1E6C4)是小鼠单克隆IgG1 κ, 在151篇文献中引用,规格为200 µg/ml
  • 针对与human来源的重组Nanog对应的氨基酸20-166
  • Nanog 抗体 (1E6C4) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 Nanog
  • 抗Nanog抗体(1E6C4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nanog (1E6C4): sc-293121免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Nanog Antibody (1E6C4) for WB applications. 的首选辅助检测试剂,这些试剂现与Nanog Antibody (1E6C4) 打包提供(请参阅下面的订购信息)。

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関連項目

Nanog抗体(1E6C4)是一种IgG1 κ小鼠单克隆Nanog抗体(也被称作Nanog抗体或Nanog同源框抗体),它通过WB、IP、IF和ELISA检测方法检测人类来源的Nanog。Nanog抗体(1E6C4)既可以作为非偶联形式的抗Nanog抗体使用,也可以作为多种偶联形式的抗Nanog抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Nanog(源自"Tir Na Nog",即神话中凯尔特人的永恒青春之地)是一种不同的同源结构域蛋白,指导未分化胚胎干细胞的多能性和分化。Nanog mRNA存在于多能的小鼠和人类细胞系中,但不存在于分化细胞中。人类Nanog蛋白与小鼠蛋白的整体氨基酸同一性为52%,在同源结构域中的同一性为85%。人类Nanog定位于基因位点12p13.31,而小鼠Nanog定位于基因位点6 F2。小鼠胚胎Nanog的表达在囊胚的内细胞团中被检测到。在未分化的NTERA-2 cl.D1胚胎癌细胞系中,通过Northern分析检测到了高水平的人类Nanog表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Nanog 抗体 (1E6C4)

sc-293121
200 µg/ml
$316.00

Nanog (1E6C4): m-IgG Fc BP-HRP 套装

sc-527320
200 µg Ab; 10 µg BP
$354.00

Nanog (1E6C4): m-IgG1 BP-HRP 套装

sc-532693
200 µg Ab; 20 µg BP
$354.00

Nanog 抗体 (1E6C4) AC

sc-293121 AC
500 µg/ml, 25% agarose
$416.00

Nanog 抗体 (1E6C4) HRP

sc-293121 HRP
200 µg/ml
$316.00

Nanog 抗体 (1E6C4) FITC

sc-293121 FITC
200 µg/ml
$330.00

Nanog 抗体 (1E6C4) PE

sc-293121 PE
200 µg/ml
$343.00

Nanog 抗体 (1E6C4) Alexa Fluor® 488

sc-293121 AF488
200 µg/ml
$357.00

Nanog 抗体 (1E6C4) Alexa Fluor® 546

sc-293121 AF546
200 µg/ml
$357.00

Nanog 抗体 (1E6C4) Alexa Fluor® 594

sc-293121 AF594
200 µg/ml
$357.00

Nanog 抗体 (1E6C4) Alexa Fluor® 647

sc-293121 AF647
200 µg/ml
$357.00

Nanog 抗体 (1E6C4) Alexa Fluor® 680

sc-293121 AF680
200 µg/ml
$357.00

Nanog 抗体 (1E6C4) Alexa Fluor® 790

sc-293121 AF790
200 µg/ml
$357.00

what is the molecular weight of Nanog antibody?

Asked by: f_mehr
Thank you for your question. As an IgG antibody, this product has an approximate molecular weight of 150.000 g/mol.
Answered by: Tech Support Europe
Date published: 2024-12-24

Has this product been discontinued? Because our nearest distributor is saying so. We are very much interested for this Antibody along with other two. Please do reply.

Asked by: Dr D Jena
Thank you for your question. This antibody is available and in stock. If your distributor is unable to get this to you, please contact an alternate distributor.
Answered by: Tech Service
Date published: 2019-01-07

I would like to use sc-293121 for some double staining. Any tips for using only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-293121, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 8
Date published: 2017-01-20
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Rated 4 out of 5 by from Specific but weak signalI’ve tested this antibody for IF on fixed cells at 1:300. I’ve tested on induced pluripotent stem cells (iPSCs) and on iPSCs-derived neurons. The signal is specific for iPSCs (negative in neurons), is well localized into the nuclei, but weak.
Date published: 2024-07-23
Rated 4 out of 5 by from ImmunofluorescenceThe antibody worked well in colorectal cancer HCT116 cells
Date published: 2022-12-01
Rated 1 out of 5 by from Does not work well on hiPS cellswith very simple procedure 4% PFA, 15 min, RT fixation hiPS cells 1:50 dilution 4℃, O/N incubation
Date published: 2021-08-05
Rated 1 out of 5 by from resullti don't know why the whole-cell bright,not noly uncleus.....
Date published: 2019-07-27
Rated 4 out of 5 by from Pluripotency factor immunofluorescenceThis antibody worked well for pluripotency factor detection by immunofluorescence.
Date published: 2018-09-25
Rated 5 out of 5 by from staining for feeder free iPSCsIt works well for feeder-free iPSCs. We have got images with little background noise.
Date published: 2018-07-11
Rated 3 out of 5 by from Ok Western Blot, Mauvais en IFJ'ai testé cette en anticorps en IF mais je n'ai eu aucun signal (1/50e ; 1/100e ; 1/200) avec un démasquage à pH 6 et pH9. En revanche, il fonctionne en Western Blot, dilution 1/500e
Date published: 2018-06-07
Rated 4 out of 5 by from ottimo funzionamentoL'anticorpo è davvero ottimo! banda netta ll'altezza di 50K
Date published: 2018-04-04
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Nanog 抗体 (1E6C4) is rated 3.9 out of 5 by 31.
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