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Myosin VIIa Antibody (C-5) - Western Blotting - Image 17383
肌球蛋白 VIIa 抗体 (C-5): sc-74516. Y79 全细胞裂解液 (A), Y79 核提取物 (B) 和小鼠睾丸组织提取物 (C) 中肌球蛋白 VIIa 表达的 Western 印迹分析.
肌球蛋白 VIIa 抗体 (C-5): sc-74516. Y79 全细胞裂解液 (A), Y79 核提取物 (B) 和小鼠睾丸组织提取物 (C) 中肌球蛋白 VIIa 表达的 Western 印迹分析.

Myosin VIIa 抗体 (C-5): sc-74516

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说明书
  • Myosin VIIa 抗体 C-5 是小鼠单克隆 IgG2a κ，Myosin VIIa抗体, 在43篇文献中引用，规格为200 µg/ml
  • 免疫human来源Myosin VIIa近N-terminus的11-70氨基酸
  • Myosin VIIa 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Myosin VIIa isoforms
  • 抗Myosin VIIa抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Myosin VIIa (C-5): sc-74516的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Myosin VIIa 抗体 (C-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Myosin VIIa 抗体 (C-5) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Myosin 抗体 包括 MRLC2, MYH, MYH1, MYH10, MYH11, MYH15, MYH2, MYH3, MYH4和MYH6

肌球蛋白7a抗体（C-5）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类肌球蛋白7a蛋白。抗肌球蛋白VIIa抗体（C-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。肌球蛋白VIIa在细胞过程中起着至关重要的作用，特别是在纤毛的形成和维护中，这对细胞信号和运动至关重要。纤毛的正常运作对于各种生理过程至关重要，包括感觉知觉和上皮表面上的液体流动。影响肌球蛋白VIIa的基因突变可导致严重的疾病，如以听力丧失和视力障碍为特征的乌谢尔综合征，这凸显了肌球蛋白VIIa在听觉和视觉系统中的重要性。对肌球蛋白VIIa在纤毛形成中的作用的研究揭示了其生物学意义，并有可能成为相关疾病的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Myosin VIIa 抗体 (C-5) 参考文献:

  1. 肌球蛋白 VII 在动态细胞粘附中的作用  |  Tuxworth, RI., et al. 2001. Curr Biol. 11: 318-29. PMID: 11267868
  2. 细胞粘附：开启对肌球蛋白 VII 的新认识。  |  Maniak, M. 2001. Curr Biol. 11: R315-7. PMID: 11369222
  3. 导致 1B 型乌谢尔综合征的肌球蛋白 VIIA 基因缺陷。  |  Weil, D., et al. 1995. Nature. 374: 60-1. PMID: 7870171
  4. 导致 Usher 1B 综合征的人类肌球蛋白 VIIA：在发育中的感觉上皮细胞中表达的一种预测的膜缔合作用运动蛋白。  |  Weil, D., et al. 1996. Proc Natl Acad Sci U S A. 93: 3232-7. PMID: 8622919
  5. 常染色体隐性遗传孤立性耳聋, DFNB2 和 Usher 1B 综合征是肌球蛋白-VIIA 基因的等位基因缺陷。  |  Weil, D., et al. 1997. Nat Genet. 16: 191-3. PMID: 9171833

订购信息

产品名称产品编号规格价格数量收藏夹

Myosin VIIa 抗体 (C-5)

sc-74516
200 µg/ml
$316.00

Myosin VIIa (C-5): m-IgG Fc BP-HRP 套装

sc-528680
200 µg Ab; 10 µg BP
$354.00

Myosin VIIa (C-5): m-IgGκ BP-HRP 套装

sc-521129
200 µg Ab, 40 µg BP
$354.00

Myosin VIIa (C-5): m-IgG2a BP-HRP 套装

sc-547143
200 µg Ab; 10 µg BP
$354.00

Myosin VIIa 抗体 (C-5) AC

sc-74516 AC
500 µg/ml, 25% agarose
$416.00

Myosin VIIa 抗体 (C-5) HRP

sc-74516 HRP
200 µg/ml
$316.00

Myosin VIIa 抗体 (C-5) FITC

sc-74516 FITC
200 µg/ml
$330.00

Myosin VIIa 抗体 (C-5) PE

sc-74516 PE
200 µg/ml
$343.00

Myosin VIIa 抗体 (C-5) Alexa Fluor® 488

sc-74516 AF488
200 µg/ml
$357.00

Myosin VIIa 抗体 (C-5) Alexa Fluor® 546

sc-74516 AF546
200 µg/ml
$357.00

Myosin VIIa 抗体 (C-5) Alexa Fluor® 594

sc-74516 AF594
200 µg/ml
$357.00

Myosin VIIa 抗体 (C-5) Alexa Fluor® 647

sc-74516 AF647
200 µg/ml
$357.00

Myosin VIIa 抗体 (C-5) Alexa Fluor® 680

sc-74516 AF680
200 µg/ml
$357.00

Myosin VIIa 抗体 (C-5) Alexa Fluor® 790

sc-74516 AF790
200 µg/ml
$357.00

What is the recommended secondary for use with this primary antibody?

Asked by: Anna99
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with our Myosin VIIa Antibody (C-5) sc-74516, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09

Myosin VIIa 抗体 (C-5): sc-74516    这个货到达上海徐汇区的货期多久？

Asked by: foul
感谢您的提问。关于中国大陆地区的订购事宜，请通过网页上的LiveChat - 中文支持、电话(+86) 021-60936351、或邮箱asia@scbio.cn，联系我们的上海分公司。 Thank you for your inquiry. For purchasing in mainland China, please contact our Shanghai office at (+86) 021-60936351 or asia@scbio.cn, or via LiveChat - Chinese Department on the website.
Answered by: Technical Support 12
Date published: 2016-12-01
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Rated 4 out of 5 by from Very specificThis antibody works well for fluorescence in tissue
Date published: 2019-01-30
Rated 4 out of 5 by from perfect for immunohisto stainingwe use the Myosin VIIa Antibody (C-5) for a lot of our studies, and never had trouble with the immunohistochemistry labelling of Myosin VIIa in the hear cells of rats
Date published: 2018-05-06
Rated 5 out of 5 by from works wellWe diluted the antibody with 1:200 at WB, a clean, powerful band was seen at 203kDa and the antibody was excellent.
Date published: 2017-11-01
Rated 5 out of 5 by from Excellent antibodyExcellent antibody. I have used it for WB and IF with perfect results.
Date published: 2017-05-25
Rated 5 out of 5 by from Superb antibody !!Very nice, highly specific western blot data; also great IHC
Date published: 2017-01-27
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-13
Rated 5 out of 5 by from I have thorougly characterized this antibody by WBI have thorougly characterized this antibody by WB, immunocytochemistry and by whole mount immunocytochemistry on rodent inner ear tissues. This antibody is very specific and showed high affinity to Myosin VIIa. It is an excellent antibody to use in mouse and rat tissues. For further information, please refer to the publication below:<br /> Grati M, Kachar B. Myosin VIIa and sans localization at stereocilia upper tip-link density implicates these Usher syndrome proteins in mechanotransduction.Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11476-81. doi:10.1073/pnas.1104161108. Epub 2011 Jun 27.<br /> PMID:21709241
Date published: 2015-10-14
Rated 1 out of 5 by from did not work in tissue after methanol fixationdid not work in tissue after methanol fixation
Date published: 2015-04-30
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Myosin VIIa 抗体 (C-5) is rated 4.4 out of 5 by 11.
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