Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Myosin Ib CRISPR/Cas9 KO Plasmid (h): sc-403530
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myosin Ib CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Myosin Ib genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myosin Ib Antibody (F-8): sc-393053
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myosin Ib CRISPR/Cas9 KO Plasmid (h) | sc-403530 | 20 µg | $397.00 |
Overview
MYO1B encodes myosin Ib, an actin-dependent, membrane-associated motor that contributes to cortical tension and dynamic remodeling of the plasma membrane. Myosin Ib participates in endocytic trafficking, membrane ruffling, and actin network organization by coupling force generation to phosphoinositide-rich membranes. Through these functions it influences cell polarity, adhesion, and migration, processes that intersect with signaling pathways regulating cytoskeletal dynamics and vesicle transport. Altered MYO1B expression or activity has been investigated in contexts of invasive cell behavior and dysregulated epithelial architecture, supporting its relevance for mechanistic studies of disease-associated remodeling of the actin cortex.
Myosin Ib CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MYO1B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MYO1B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MYO1B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Myosin Ib protein expression.
This CRISPR knockout system enables efficient generation of MYO1B-deficient cell models for investigation of Myosin Ib signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MYO1B exon(s) critical for Myosin Ib function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MYO1B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Myosin Ib CRISPR/Cas9 KO Plasmid (h) and Myosin Ib CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MYO1B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Myosin Ib HDR Plasmid (h) and Myosin Ib HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MYO1B homology arms to support homology-directed repair at defined MYO1B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.