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Myosin Ib Antibody (F-8) - Western Blotting - Image 291999 — 肌球蛋白 Ib 抗体 (F-8): sc-393053. 未转染 293T: sc-117752 (A), 转染人肌球蛋白 Ib 的 293T: sc-116536 (B) 和 Jurkat (C) 全细胞裂解液中肌球蛋白 Ib 表达的 Western 印迹分析.

肌球蛋白 Ib 抗体 (F-8): sc-393053. 未转染 293T: sc-117752 (A), 转染人肌球蛋白 Ib 的 293T: sc-116536 (B) 和 Jurkat (C) 全细胞裂解液中肌球蛋白 Ib 表达的 Western 印迹分析.

Myosin Ib 抗体 (F-8): sc-393053

Name: Myosin Ib抗体 (F-8)
Brand: SCBT
SKU: sc-393053
Price: 316.0 USD
Availability: InStock

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说明书

Myosin Ib 抗体 F-8 是小鼠单克隆 IgG₁ κ，Myosin Ib抗体, 在4篇文献中引用，规格为200 µg/ml
免疫原氨基酸序列1067-1136位于human物种的Myosin Ib的C-terminus
Myosin Ib 抗体 (F-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Myosin Ib; 也和以下物种反应，包括：和 equine, canine, bovine and porcine
抗Myosin Ib抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取Myosin Ib (F-8): sc-393053的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是Myosin Ib Antibody (F-8) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与Myosin Ib Antibody (F-8) 打包提供（请参阅下面的订购信息）。

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Myosin Ib 抗体 (F-8) 参考文献:

溶组织阿米巴的肌球蛋白IB参与人类红细胞的吞噬作用。 | Voigt, H., et al. 1999. J Cell Sci. 112 (Pt 8): 1191-201. PMID: 10085254
在活体溶组织阿米巴中肌球蛋白IB的过度表达会增强细胞质粘性并降低吞噬作用。 | Marion, S., et al. 2004. J Cell Sci. 117: 3271-9. PMID: 15226399
哺乳动物 I 类肌球蛋白 Myo1b 的转导区环路 1 调节肌动蛋白的亲和力, ATP 酶活性和核苷酸的获取。 | Clark, R., et al. 2005. J Biol Chem. 280: 30935-42. PMID: 15980431
裂殖酵母肌球蛋白 I 在富固醇膜域组织中的作用 | Takeda, T. and Chang, F. 2005. Curr Biol. 15: 1331-6. PMID: 16051179
细胞核中的肌动蛋白和肌球蛋白 I：下一步是什么？ | de Lanerolle, P., et al. 2005. Nat Struct Mol Biol. 12: 742-6. PMID: 16142228
在组织溶解线虫吞噬作用的早期阶段通过肌球蛋白 IB 进行信号转导和细胞骨架活动：一种蛋白质组学方法。 | Marion, S., et al. 2005. Cell Microbiol. 7: 1504-18. PMID: 16153248
肌球蛋白 Ib 可调节多囊分拣内体的形态和蛋白质运输。 | Salas-Cortes, L., et al. 2005. J Cell Sci. 118: 4823-32. PMID: 16219689
肌球蛋白IB与网柄菌中的肌动蛋白波结合需要肌球蛋白尾部结合质膜的位点和结合肌动蛋白的区域。 | Brzeska, H., et al. 2014. PLoS One. 9: e94306. PMID: 24747353
棘阿米巴肌球蛋白IB的重链是肌球蛋白样和非肌球蛋白样序列的融合。 | Jung, G., et al. 1987. Proc Natl Acad Sci U S A. 84: 6720-4. PMID: 3477803
靶向MYO1B通过抑制食管鳞状细胞癌中的SNAI2/cyclin D1信号传导来抑制肿瘤发生。 | Li, SH., et al. 2022. J Cell Physiol. 237: 3671-3686. PMID: 35861939
Myo1b通过抑制结肠直肠癌中HIF-1α的自噬降解来促进肿瘤进展和血管生成。 | Chen, YH., et al. 2022. Cell Death Dis. 13: 939. PMID: 36347835
MYO1B作为槟榔碱相关口腔癌的预后生物标志物和治疗靶点。 | Sun, Z., et al. 2023. Mol Carcinog. 62: 920-939. PMID: 37014156

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
Myosin Ib 抗体 (F-8)	sc-393053	200 µg/ml	$316.00
Myosin Ib (F-8): m-IgG Fc BP-HRP 套装	sc-530341	200 µg Ab; 10 µg BP	$354.00
Myosin Ib (F-8): m-IgGκ BP-HRP 套装	sc-523787	200 µg Ab, 40 µg BP	$354.00
Myosin Ib (F-8): m-IgG₁ BP-HRP 套装	sc-543999	200 µg Ab; 20 µg BP	$354.00
Myosin Ib 抗体 (F-8) AC	sc-393053 AC	500 µg/ml, 25% agarose	$416.00
Myosin Ib 抗体 (F-8) HRP	sc-393053 HRP	200 µg/ml	$316.00
Myosin Ib 抗体 (F-8) FITC	sc-393053 FITC	200 µg/ml	$330.00
Myosin Ib 抗体 (F-8) PE	sc-393053 PE	200 µg/ml	$343.00
Myosin Ib 抗体 (F-8) Alexa Fluor^® 488	sc-393053 AF488	200 µg/ml	$357.00
Myosin Ib 抗体 (F-8) Alexa Fluor^® 546	sc-393053 AF546	200 µg/ml	$357.00
Myosin Ib 抗体 (F-8) Alexa Fluor^® 594	sc-393053 AF594	200 µg/ml	$357.00
Myosin Ib 抗体 (F-8) Alexa Fluor^® 647	sc-393053 AF647	200 µg/ml	$357.00
Myosin Ib 抗体 (F-8) Alexa Fluor^® 680	sc-393053 AF680	200 µg/ml	$357.00
Myosin Ib 抗体 (F-8) Alexa Fluor^® 790	sc-393053 AF790	200 µg/ml	$357.00

We tried to use this antibody to do immunofluorescence (IF) on various mouse samples and it didn't work well, it has a very bad signal:noise ratio. Do you have alternative antibodies that work for IF in mouse cells?

Asked by: FC11

Thank you for question. We are sorry to hear that this Myosin Ib antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department, we are happy to help you troubleshoot your product. Santa Cruz Biotechnology does warranty our Myosin Ib antibody for one year from the date of purchase. For an alternative antibody for Myosin Ib, we do not offer any other clones of this, at this time. Please check this link for further updates: https://www.scbt.com/browse/antibodies-Primary-Antibodies-and-ImmunoCruz-sup-reg-sup-Conjugates-structural-proteins-antibodies-myosin-ib-Antibodies/_/N-5qmmt4.

Answered by: Technical Service 4

Date published: 2020-11-16

Are there any issues with using sc-393053: Myosin Ib (F-8) mouse monoclonal primary antibody on mouse samples?

Asked by: jenniferc

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-393053 conjugates.

Answered by: Technical Support

Date published: 2017-03-07

Rated 5 out of 5 by DefinitelyNotMatt from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.

Date published: 2017-02-03

Rated 5 out of 5 by JT from Great band strength in Western Blot withGreat band strength in Western Blot with transfected 293T lysates and Jurkat cell lysates -SCBT QC

Date published: 2014-10-25

Myosin Ib 抗体 (F-8) is rated 5.0 out of 5 by 2.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	MYO1B	Entrez Gene ID: 4430	2q32.3	XM_005246572, XM_017004156, XM_017004157, XM_017004158, NM_001130158, NM_001161819, NM_001330237, NM_001330238, NM_012223	UniProt ID: O43795	n/a
Mouse	Myo1b	Entrez Gene ID: 17912	1 C1.1	XM_006495764, XM_006495765, XM_006495766, XM_006495767, XM_006495769, XM_017319213, NM_001161817, NM_001290982, NM_010863	UniProt ID: P46735	N/A

Myosin Ib 抗体 (F-8): sc-393053

Myosin Ib 抗体 (F-8) 参考文献:

Myosin Ib 抗体 (F-8) 规格

替代产品名称: Myosin Ib

产品克隆名称: F-8

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 1067-1136

抗原表位靶标: Myosin Ib

抗原表位种属: human

产品反应性/检测: Myosin Ib

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: equine, canine, bovine and porcine

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 4篇各种科学出版物的引文

Myosin Ib 抗体 (F-8)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin