Date published: 2025-9-6
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MYH3 Antibody (F1.652) - Western Blotting - Image 402180
MYH3 抗体 (F1. 652) Alexa Fluor® 647: sc-53091 AF647. L8 全细胞裂解液 (A) 和大鼠胚胎组织提取物 (B) 中 MYH3 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
MYH3 抗体 (F1. 652) Alexa Fluor® 647: sc-53091 AF647. L8 全细胞裂解液 (A) 和大鼠胚胎组织提取物 (B) 中 MYH3 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

MYH3 抗体 (F1.652): sc-53091

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说明书
  • MYH3抗体(F1.652)是小鼠单克隆IgG1 κ, 在50篇文献中引用，规格为200 µg/ml
  • 针对human来源的胎儿骨骼肌肌球蛋白
  • 抗-MYH3 抗体 (F1.652) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和rabbit 来源的 myosin heavy chain encoded by MYH3
  • 抗MYH3抗体（F1.652）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 和ELISA
  • 关于如何获取MYH3 (F1.652): sc-53091的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 MYH3 抗体 (F1.652) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MYH3 抗体 (F1.652) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Myosin 抗体 包括 MRLC2, MYH, MYH1, MYH10, MYH11, MYH15, MYH2, MYH3, MYH4和MYH6

MYH3抗体（F1.652）是一种IgG1 κ小鼠单克隆MYH3抗体，可通过WB、IP、IF和IHC(P)检测小鼠、大鼠和人源的由MYH3编码的肌球蛋白重链。MYH3抗体（F1.652）既可作为非偶联抗MYH3抗体形式提供，也可作为多种偶联形式的抗MYH3抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌动蛋白是一种在所有真核细胞中表达的高度保守的蛋白质。肌动蛋白丝可以形成稳定和不稳定的结构，并且是微绒毛和肌肉细胞收缩装置的关键组成部分。肌球蛋白是由两条重链（MHC）和四条轻链（MLC）组成的六聚体，与肌动蛋白相互作用以产生多种细胞运动所需的力，包括细胞质分裂、吞噬作用和肌肉收缩。MYH3（肌球蛋白重链3），也称为肌肉胚胎肌球蛋白重链或SMHCE，是一种定位于肌原纤维粗丝中的由1940个氨基酸组成的蛋白质。虽然在胎儿骨骼肌中高度表达，但在成人骨骼肌中几乎检测不到MYH3。定位于人类染色体17p13.1的编码MYH3的基因缺陷是导致远端关节弯曲2B型（DA2B）的原因。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MYH3 抗体 (F1.652) 参考文献:

  1. 胚胎肌球蛋白重链（MYH3）的突变会导致弗里曼-谢尔顿综合征（Freeman-Sheldon Syndrome）和谢尔顿-霍尔综合征（Sheldon-Hall Syndrome）。  |  Toydemir, RM., et al. 2006. Nat Genet. 38: 561-5. PMID: 16642020
  2. 鉴定人类肌球蛋白重链的三种发育受控异构体  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
  3. 平滑肌肌球蛋白由同二聚体重链组成。  |  Kelley, CA., et al. 1992. J Biol Chem. 267: 2127-30. PMID: 1733920
  4. 生成全长的人类围产期肌球蛋白重链编码 cDNA。  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
  5. MYH3的功能性调节变异会影响猪的肌肉纤维类型组成和肌内脂肪含量。  |  Cho, IC., et al. 2019. PLoS Genet. 15: e1008279. PMID: 31603892
  6. 扩展MYH3相关骨骼疾病的突变和表型谱。  |  Zhao, S., et al. 2022. NPJ Genom Med. 7: 11. PMID: 35169139
  7. FOXO1通过靶向MYH3来调节牛骨骼肌细胞的分化。  |  Jiang, C., et al. 2024. Int J Biol Macromol. 260: 129643. PMID: 38253149
  8. 人类围产期肌球蛋白重链转录本的特征。  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
  9. 调节血管平滑肌细胞的分化。  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
  10. 哺乳动物骨骼肌发育过程中依次表达的三条慢肌球蛋白重链  |  Hughes, SM., et al. 1993. Dev Biol. 158: 183-99. PMID: 7687223
  11. 肌球蛋白超家族的系统发育分析。  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
  12. 哺乳动物肌球蛋白重链基因家族。  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733

订购信息

产品名称产品编号规格价格数量收藏夹

MYH3 抗体 (F1.652)

sc-53091
200 µg/ml
$316.00

MYH3 (F1.652): m-IgG Fc BP-HRP 套装

sc-526766
200 µg Ab; 10 µg BP
$354.00

MYH3 (F1.652): m-IgG1 BP-HRP 套装

sc-532139
200 µg Ab; 20 µg BP
$354.00

MYH3 抗体 (F1.652) AC

sc-53091 AC
500 µg/ml, 25% agarose
$416.00

MYH3 抗体 (F1.652) HRP

sc-53091 HRP
200 µg/ml
$316.00

MYH3 抗体 (F1.652) FITC

sc-53091 FITC
200 µg/ml
$330.00

MYH3 抗体 (F1.652) PE

sc-53091 PE
200 µg/ml
$343.00

MYH3 抗体 (F1.652) Alexa Fluor® 488

sc-53091 AF488
200 µg/ml
$357.00

MYH3 抗体 (F1.652) Alexa Fluor® 546

sc-53091 AF546
200 µg/ml
$357.00

MYH3 抗体 (F1.652) Alexa Fluor® 594

sc-53091 AF594
200 µg/ml
$357.00

MYH3 抗体 (F1.652) Alexa Fluor® 647

sc-53091 AF647
200 µg/ml
$357.00

MYH3 抗体 (F1.652) Alexa Fluor® 680

sc-53091 AF680
200 µg/ml
$357.00

MYH3 抗体 (F1.652) Alexa Fluor® 790

sc-53091 AF790
200 µg/ml
$357.00

MYH3 抗体 (F1.652) B

sc-53091 B
200 µg/ml
$320.00

Do you sell biotinylated Anti-MYH3 antibody? If not, do you have recommendations for how one can biotinylate this antibody?

Asked by: brandonh
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-02

Is the antigen for this antibody for the entire 1940 AA sequence? I need to match the sequence used to equine sequence to see if I can use it. thank you,

Asked by: Kgardner
Thank you for your question. This mouse monoclonal antibody is not raised against an amino acid sequence, but rather is raised against a preparation of fetal skeletal muscle myosin of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. Most product citations use this antibody in mouse and rat samples, and we are not aware of any product citations in equine samples at this time. Reactivity with equine would have to be determined experimentally.
Answered by: Technical Support
Date published: 2020-02-12

Do you use Heat induce epitiope retrieval for MYH3 (F1.652):sc-53091.

Asked by: MIGUEL VARGAS
Thank you for your question. Yes, we recommend antigen retrieval using sodium citrate and heat for sc-53091.
Answered by: Technical Support
Date published: 2018-12-14

Can this antibody be used in IHC with paraffin-embedded tissue sections?

Asked by: AbPolly
Thank you for your inquiry. This antibody has not been validated in house for use in IHC(P). For any publications in this application and further details, please contact our Technical Service Department.
Answered by: Technical Support 13
Date published: 2018-07-23
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Rated 5 out of 5 by from Sensitive antibodyThis MYH3 antibody is easy to use without any need to modify the protocol. It is specific and sensitive to the target protein at 200kda by 1:500.
Date published: 2017-07-29
Rated 5 out of 5 by from clear bandStrong and clear band has been gotten by this specific antibody for WB anyasis.
Date published: 2017-05-28
Rated 5 out of 5 by from Strong western dataNice product for western blot applications, low background
Date published: 2017-01-03
Rated 5 out of 5 by from A perfect choice for your Western blot!This antibody is your best choice for detecing the myosin heavy chain. It is reactive in several different species, but provides clear specific bands with each sample. It has been published with several times!
Date published: 2016-12-28
Rated 5 out of 5 by from signal via Western blot analysis of MHC expressionsignal via Western blot analysis of MHC expression in L8 whole cell lysate. -SCBT QC
Date published: 2015-01-06
Rated 5 out of 5 by from ICC publication using mouse satellite cellICC publication using mouse satellite cell from hind limb skeletal muscles -SCBT Publication Review
Date published: 2013-06-05
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MYH3 抗体 (F1.652) is rated 5.0 out of 5 by 6.
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