Date published: 2025-9-6

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Mucin 15/MUC15 抗体 (H-5): sc-365746

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说明书
  • Mucin 15/MUC15 抗体 H-5 是小鼠单克隆 IgG1 (kappa轻链),Mucin 15/MUC15抗体,规格为200 µg/ml
  • 针对Mucin 15的mouse起源的N-terminal细胞外域内的氨基酸23-232
  • Mucin 15/MUC15 抗体 (H-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Mucin 15
  • 抗Mucin 15/MUC15抗体(H-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mucin 15/MUC15 (H-5): sc-365746免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Mucin 15/MUC15 Antibody (H-5) for WB applications. 的首选辅助检测试剂,这些试剂现与Mucin 15/MUC15 Antibody (H-5) 打包提供(请参阅下面的订购信息)。

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    関連項目

    Mucin 15抗体(H-5)是一种IgG1 κ小鼠单克隆Mucin 15抗体(也称为mucin 15细胞表面相关抗体或MUC15抗体),它通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类来源的Mucin 15蛋白。Mucin 15抗体(H-5)既以非共轭的抗Mucin 15抗体形式存在,也有多种共轭形式的抗Mucin 15抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®共轭物。粘蛋白(mucins)是一类高度糖基化的分泌蛋白,其基本结构由可变数量的串联重复序列(VNTRs)组成。膜结合型和分泌型粘蛋白是糖萼(多糖生物膜)中的高分子量糖蛋白,可保护粘膜上皮免受颗粒物和微生物的侵害。上皮粘蛋白是大型分泌型和细胞表面糖蛋白,对粘附调节、信号传导和上皮细胞保护至关重要。重复序列的数量具有高度多态性,并在不同等位基因之间有所变化。粘蛋白家族包括Mucins 1-4、Mucin 5(AC和B)、Mucins 6-8、Mucins 11-13以及Mucins 15-17。Mucin 15参与细胞与细胞外基质的粘附。它主要在前列腺、睾丸、肺、脾、胸腺、卵巢、小肠、结肠、骨髓、淋巴结、肺和外周血白细胞中表达。Mucin 15是一种高度糖基化的蛋白质。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Mucin 15/MUC15 抗体 (H-5) 参考文献:

    1. 人类粘蛋白基因的结构组织和分类。  |  Moniaux, N., et al. 2001. Front Biosci. 6: D1192-206. PMID: 11578969
    2. 慢性阻塞性肺病中粘蛋白载体的表达。  |  Leikauf, GD., et al. 2002. Chest. 121: 166S-182S. PMID: 12010847
    3. 新型细胞膜相关粘蛋白 MUC15 的分离和特征描述。  |  Pallesen, LT., et al. 2002. Eur J Biochem. 269: 2755-63. PMID: 12047385
    4. 大肠癌中的粘蛋白和粘蛋白结合蛋白。  |  Byrd, JC. and Bresalier, RS. 2004. Cancer Metastasis Rev. 23: 77-99. PMID: 15000151
    5. 人胰腺肿瘤粘蛋白 cDNA 的克隆和测序。  |  Lan, MS., et al. 1990. J Biol Chem. 265: 15294-9. PMID: 2394722

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Mucin 15/MUC15 抗体 (H-5)

    sc-365746
    200 µg/ml
    $316.00

    Mucin 15/MUC15 (H-5): m-IgG Fc BP-HRP 套装

    sc-527396
    200 µg Ab; 10 µg BP
    $354.00

    Mucin 15/MUC15 (H-5): m-IgG1 BP-HRP 套装

    sc-532769
    200 µg Ab; 20 µg BP
    $354.00

    Mucin 15/MUC15 抗体 (H-5) AC

    sc-365746 AC
    500 µg/ml, 25% agarose
    $416.00

    Mucin 15/MUC15 抗体 (H-5) HRP

    sc-365746 HRP
    200 µg/ml
    $316.00

    Mucin 15/MUC15 抗体 (H-5) FITC

    sc-365746 FITC
    200 µg/ml
    $330.00

    Mucin 15/MUC15 抗体 (H-5) PE

    sc-365746 PE
    200 µg/ml
    $343.00

    Mucin 15/MUC15 抗体 (H-5) Alexa Fluor® 488

    sc-365746 AF488
    200 µg/ml
    $357.00

    Mucin 15/MUC15 抗体 (H-5) Alexa Fluor® 546

    sc-365746 AF546
    200 µg/ml
    $357.00

    Mucin 15/MUC15 抗体 (H-5) Alexa Fluor® 594

    sc-365746 AF594
    200 µg/ml
    $357.00

    Mucin 15/MUC15 抗体 (H-5) Alexa Fluor® 647

    sc-365746 AF647
    200 µg/ml
    $357.00

    Mucin 15/MUC15 抗体 (H-5) Alexa Fluor® 680

    sc-365746 AF680
    200 µg/ml
    $357.00

    Mucin 15/MUC15 抗体 (H-5) Alexa Fluor® 790

    sc-365746 AF790
    200 µg/ml
    $357.00

    Are there any issues with using sc-365746: Mucin 15 (H-5) mouse monoclonal primary antibody on mouse samples?

    Asked by: AbPolly
    Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-365746 conjugates.
    Answered by: Technical Support
    Date published: 2017-03-07
    • y_2025, m_8, d_28, h_5CST
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    Rated 1 out of 5 by from It does not work in IF on FFPE tissue sectionsI used this Ab on paraffin embedded tissue sections at two dilutions (1:100 and 1:200) following the SC protocol for IF on such samples. I used a positive control, anti-MUC2, a sample previously and kindly provided by SC, which works well. It may be a matter of low expression
    Date published: 2022-10-19
    Rated 2 out of 5 by from Not cross-reactivity with bovineWe have tried the antibody on cells isolated from bovine milk and we were unable to detect any binding; thus, we concluded that the antibody does not work on the extracellular region of bovine MUC15.
    Date published: 2017-05-22
    Rated 5 out of 5 by from Signal via western blot analysis of MucinSignal via western blot analysis of Mucin 15 expression in non-transfected: sc-117752 (A) and mouse Mucin 15 transfected: sc-127174 (B) 293T whole cell lysates. -SCBT QC
    Date published: 2013-09-10
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    Mucin 15/MUC15 抗体 (H-5) is rated 2.7 out of 5 by 3.
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