Date published: 2025-9-7

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MMP9 抗体 (E-11): sc-393859

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说明书
  • MMP9 抗体 E-11 是小鼠单克隆 IgG2b, 在166篇文献中引用,提供 200 µg/ml
  • 特异性抗原位于human物种的MMP-9的C-terminus的氨基酸678-707之间
  • 抗-MMP9 抗体 (E-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 MMP-9
  • 抗MMP9抗体(E-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MMP9 (E-11): sc-393859免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 MMP9 抗体 (E-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MMP9 抗体 (E-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MMP-9抗体(E-11)是一种IgG2b小鼠单克隆MMP-9抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的MMP-9。MMP-9抗体(E-11)既可作为非偶联抗MMP-9抗体形式,也可作为多种偶联形式的抗MMP-9抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。基质金属蛋白酶(MMP)是一组负责降解细胞外基质成分的肽酶,包括胶原蛋白、明胶、纤维连接蛋白、层粘连蛋白和蛋白聚糖。MMP基因的转录被佛波酯、脂多糖(LPS)或葡萄球菌肠毒素B(SEB)差异激活。MMP催化需要钙和锌。MMP-9(也称为92 kDa IV型胶原酶或明胶酶B)已被证明与MMP-1(也称为间质胶原酶、成纤维细胞胶原酶或胶原酶-1)、半胱氨酸蛋白酶协同作用降解骨胶原蛋白,并可能在骨破骨细胞吸收中发挥作用。MMP-1被p53下调,p53表达的异常可能通过调节MMP-1表达导致类风湿关节炎的关节降解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MMP9 抗体 (E-11)

sc-393859
200 µg/ml
$316.00

MMP9 (E-11): m-IgG Fc BP-HRP 套装

sc-526270
200 µg Ab; 10 µg BP
$354.00

MMP9 (E-11): m-IgG2b BP-HRP 套装

sc-549704
200 µg Ab; 10 µg BP
$354.00

MMP9 抗体 (E-11) AC

sc-393859 AC
500 µg/ml, 25% agarose
$416.00

MMP9 抗体 (E-11) HRP

sc-393859 HRP
200 µg/ml
$316.00

MMP9 抗体 (E-11) FITC

sc-393859 FITC
200 µg/ml
$330.00

MMP9 抗体 (E-11) PE

sc-393859 PE
200 µg/ml
$343.00

MMP9 抗体 (E-11) Alexa Fluor® 488

sc-393859 AF488
200 µg/ml
$357.00

MMP9 抗体 (E-11) Alexa Fluor® 546

sc-393859 AF546
200 µg/ml
$357.00

MMP9 抗体 (E-11) Alexa Fluor® 594

sc-393859 AF594
200 µg/ml
$357.00

MMP9 抗体 (E-11) Alexa Fluor® 647

sc-393859 AF647
200 µg/ml
$357.00

MMP9 抗体 (E-11) Alexa Fluor® 680

sc-393859 AF680
200 µg/ml
$357.00

MMP9 抗体 (E-11) Alexa Fluor® 790

sc-393859 AF790
200 µg/ml
$357.00

MMP9 (E-11) 中和勝肽

sc-393859 P
100 µg/0.5 ml
$68.00

The MMP-9 band was observed at a size lower than that of MMP-1 (SC-58377). Is the size on the data sheet correct?

Asked by: ssy33144
Thank you for your question. Please contact our technical service directly so we can assist you with your experiment.
Answered by: Tech Support Europe
Date published: 2020-06-18

Hi, I have purchased this MMP9 monoclone antibody. I extracted total protein from HTR8 cell and loaded 50ug protein for western blot. Although I have tried for 3times, No signal was detected on the PVDF membrane. Could anyone help me with that please

Asked by: YipingYi
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

Which secondary antibody should I use with this for Westerns?

Asked by: Anna99
Try our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 1 out of 5 by from Did not work wellAb did not work well on neonatal mouse lungs for WB.
Date published: 2024-12-10
Rated 4 out of 5 by from GoodIt is good for Western blot analysis. Dilution user 1:1000
Date published: 2022-04-07
Rated 4 out of 5 by from バンドが検出されます。WBで使用しました。非特異的なバンドが検出されますが問題ないと思います。目的のバンドはよりシャープなバンドとして検出されました。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 3 out of 5 by from Western BlotI think the Band was found in 68 kD.... Could this happen?
Date published: 2019-01-09
Rated 4 out of 5 by from Good Ab for IHC staining of human MMP9 in FPPEGood Ab for IHC staining of MMP9 in FPPE samples of human tissue. Clean and specific staining
Date published: 2018-11-23
Rated 5 out of 5 by from Great product!!It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from UPENNGα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
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MMP9 抗体 (E-11) is rated 4.4 out of 5 by 38.
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