MMP-12 Antibody (G-2): sc-390863

MMP-12 Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects MMP-12 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MMP-12 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MMP-12, also known as macrophage metalloelastase, plays a crucial role in the degradation of elastin, a key component of the extracellular matrix, which is vital for maintaining the structural integrity and elasticity of tissues. MMP-12′s ability to degrade elastin is particularly important in inflammatory disorders, where MMP-12 may facilitate macrophage migration through the epidermal and vascular basement membranes, contributing to tissue remodeling and repair processes. Additionally, MMP-12 gene transcription is differentially regulated by various stimuli such as phorbol ester, lipopolysaccharide, and staphylococcal enterotoxin B, highlighting MMP-12′s involvement in diverse physiological and pathological processes. MMP-12′s enzymatic activity depends on calcium and zinc ions, which are essential for catalytic function, further underscoring MMP-12′s significance in extracellular matrix dynamics and immune responses.

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MMP-12 Antibody (G-2) References:

1. Enhanced expression of human metalloelastase (MMP-12) in cutaneous granulomas and macrophage migration.  |  Vaalamo, M., et al. 1999. J Invest Dermatol. 112: 499-505. PMID: 10201535
2. Molecular cloning, chromosomal localization, and bacterial expression of a murine macrophage metalloelastase.  |  Shapiro, SD., et al. 1992. J Biol Chem. 267: 4664-71. PMID: 1537850
3. Macrophage metalloelastase (MMP-12) as a target for inflammatory respiratory diseases.  |  Lagente, V., et al. 2009. Expert Opin Ther Targets. 13: 287-95. PMID: 19236151
4. MMP-12 and TIMP Behavior in Symptomatic and Asymptomatic Critical Carotid Artery Stenosis.  |  Del Porto, F., et al. 2017. J Stroke Cerebrovasc Dis. 26: 334-338. PMID: 27746079
5. Correlations of MMP-1, MMP-3, and MMP-12 with the degree of atherosclerosis, plaque stability and cardiovascular and cerebrovascular events.  |  Hu, W., et al. 2018. Exp Ther Med. 15: 1994-1998. PMID: 29434795
6. Estimation of salivary matrix metalloproteinases- 12 (MMP- 12) levels among patients presenting with oral submucous fibrosis and oral squamous cell carcinoma.  |  Saleem, Z., et al. 2021. BMC Oral Health. 21: 205. PMID: 33892690
7. RSV infection-elicited high MMP-12-producing macrophages exacerbate allergic airway inflammation with neutrophil infiltration.  |  Makino, A., et al. 2021. iScience. 24: 103201. PMID: 34703996
8. Circulating MMP-12 as Potential Biomarker in Evaluating Disease Severity and Efficacy of Sublingual Immunotherapy in Allergic Rhinitis.  |  Zhou, Y., et al. 2022. Mediators Inflamm. 2022: 3378035. PMID: 35733520
9. Cloning and characterization of a unique elastolytic metalloproteinase produced by human alveolar macrophages.  |  Shapiro, SD., et al. 1993. J Biol Chem. 268: 23824-9. PMID: 8226919
10. Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study.  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
11. Matrix metalloproteinases: a review.  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
12. Expression of several matrix metalloproteinase genes in human monocytic cells.  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MMP-12 Antibody (G-2) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-MMP-12 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MMP-12 Antibody (G-2) Specifications

Alternate Product Name: MMP-12

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 438-453

Epitope Target: MMP-12

Epitope Species: mouse

Product Reactivity/Detection: MMP-12

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for MMP-12 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin