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MCM7 Antibody (0.N.194) - Western Blotting - Image 356616
MCM7 Antibody (0.N.194): sc-71550. Western blot analysis of MCM7 expression in HL-60 (A), A549 (B), AMJ2-C8 (C), BC3H1 (D), C6 (E) and L8 (F) whole cell lysates.
MCM7 Antibody (0.N.194): sc-71550. Western blot analysis of MCM7 expression in HL-60 (A), A549 (B), AMJ2-C8 (C), BC3H1 (D), C6 (E) and L8 (F) whole cell lysates.

MCM7 Antibody (0.N.194): sc-71550

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Datasheets
  • MCM7 Antibody (0.N.194) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
  • raised against full length MCM7 of human origin
  • recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See MCM7 (141.2): sc-9966 for MCM7 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCM7 Antibody (0.N.194) for WB and IHC(P) applications. These reagents are now offered in bundles with MCM7 Antibody (0.N.194) (see ordering information below).
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Additional MCM Antibodies including MCM2, MCM3, MCM4, MCM5 and MCM6

MCM7 Antibody (0.N.194) is a mouse monoclonal IgG1 antibody that detects MCM7 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MCM7 antibody (0.N.194) is part of the mini-chromosome maintenance (MCM) family, which plays a crucial role in the regulation of DNA replication, ensuring that DNA is replicated only once during each cell cycle. MCM7, along with MCM2, MCM3, MCM4, MCM5, and MCM6, contains an ATP-binding motif that facilitates the ATP-dependent opening of double-stranded DNA, a vital step in the initiation of DNA replication. Expression of MCM proteins, including MCM7, peaks during the transition from the G1 to S phase of the cell cycle, highlighting their importance in cell proliferation. MCM7 interacts with other MCM proteins to form a complex that exhibits DNA helicase activity, which is essential for unwinding DNA strands during replication. Understanding MCM7 function and regulation is critical, as dysregulation can lead to genomic instability and is often associated with various cancers.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

MCM7 Antibody (0.N.194) References:

  1. Minichromosome maintenance protein MCM7 is a direct target of the MYCN transcription factor in neuroblastoma.  |  Shohet, JM., et al. 2002. Cancer Res. 62: 1123-8. PMID: 11861392
  2. EZH2, Ki-67 and MCM7 are prognostic markers in prostatectomy treated patients.  |  Laitinen, S., et al. 2008. Int J Cancer. 122: 595-602. PMID: 17943722
  3. Cyclin A promotes S-phase entry via interaction with the replication licensing factor Mcm7.  |  Chibazakura, T., et al. 2011. Mol Cell Biol. 31: 248-55. PMID: 21078875
  4. Prognostic significance of MCM7 expression in the bronchial brushings of patients with non-small cell lung cancer (NSCLC).  |  Liu, YZ., et al. 2012. Lung Cancer. 77: 176-82. PMID: 22456526
  5. Unique pattern of ORC2 and MCM7 localization during DNA replication licensing in the mouse zygote.  |  Ortega, MA., et al. 2012. Biol Reprod. 87: 62. PMID: 22674395
  6. Differential regulation of MCM7 and its intronic miRNA cluster miR-106b-25 during megakaryopoiesis induced polyploidy.  |  Haldar, S., et al. 2014. RNA Biol. 11: 1137-47. PMID: 25483046
  7. RNAi-mediated knockdown of MCM7 gene on CML cells and its therapeutic potential for leukemia.  |  Tian, L., et al. 2017. Med Oncol. 34: 21. PMID: 28058629
  8. Centrosomal MCM7 strengthens the Cep68-VHL interaction and excessive MCM7 leads to centrosome splitting resulting from increase in Cep68 ubiquitination and proteasomal degradation.  |  Kong, L., et al. 2017. Biochem Biophys Res Commun. 489: 497-502. PMID: 28578000
  9. Arsenic trioxide inhibits liver cancer stem cells and metastasis by targeting SRF/MCM7 complex.  |  Wang, HY., et al. 2019. Cell Death Dis. 10: 453. PMID: 31186405
  10. Correction to: MCM7 promotes cancer progression through cyclin D1-dependent signaling and serves as a prognostic marker for patients with hepatocellular carcinoma.  |  Qu, K., et al. 2022. Cell Death Dis. 13: 950. PMID: 36357373

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

MCM7 Antibody (0.N.194)

sc-71550
200 µg/ml
$322.00

MCM7 Antibody (0.N.194): m-IgG Fc BP-HRP Bundle

sc-537202
200 µg Ab; 10 µg BP
$361.00

MCM7 Antibody (0.N.194): m-IgGκ BP-HRP Bundle

sc-534412
200 µg Ab; 40 µg BP
$361.00

MCM7 Antibody (0.N.194): m-IgG1 BP-HRP Bundle

sc-545075
200 µg Ab; 20 µg BP
$361.00

What blocking solution should I use with MCM7 (0.N.194): sc-71550 monoclonal antibody for Western blot?

Asked by: Randy McDonald
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Robust bands observed in western blot analysisRobust bands observed in western blot analysis of MCM7 expression in non-transfected 293T, human MCM7 transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-19
Rated 4 out of 5 by from Slightly faint bands present in western blotSlightly faint bands present in western blot using COLO 320DM, H4 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-08-05
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MCM7 Antibody (0.N.194) is rated 4.0 out of 5 by 2.
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