Mcl-1 Antibody (MCL-2601): sc-69840

Mcl-1 Antibody (MCL-2601) is a mouse monoclonal IgG1 kappa light chain antibody raised against recombinant full-length human Mcl-1, suitable for detecting Mcl-1 from mouse, rat, and human origins via western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. Mcl-1, also called Myeloid cell leukemia-1, Mcl1, and Gm52627, is a critical member of the B-cell lymphoma 2 (Bcl-2) family of proteins that regulate apoptosis, predominantly localized to the mitochondria, where Mcl-1 plays a vital role in inhibiting cell death by binding to and neutralizing pro-apoptotic proteins like Bak and Bax, thus maintaining mitochondrial integrity and preventing the release of cytochrome c, a key step in the intrinsic apoptotic pathway. The mitochondrial localization of Mcl-1 allows direct regulation of mitochondrial outer membrane permeabilization, making Mcl-1 essential for cell survival and homeostasis, especially in early hematopoietic progenitors. Alterations in Mcl-1 expression or mitochondrial localization can disrupt the balance between cell survival and apoptosis, contributing to the development of various diseases, including cancer, where Mcl-1 overexpression is associated with tumor progression and resistance to chemotherapy. The stability and function of Mcl-1 are tightly regulated through post-translational modifications, such as phosphorylation by glycogen synthase kinase 3 (GSK3), which targets Mcl-1 for ubiquitination and proteasomal degradation. Understanding the cellular localization and regulatory mechanisms of Mcl-1 is crucial for apoptosis research and therapeutic intervention, making anti-Mcl-1 antibody (MCL-2601) an invaluable tool for scientists investigating the molecular underpinnings of cell death and survival.

The preferred Mcl-1 antibody is Mcl-1 Antibody (22): sc-12756.