Mcl-1 Antibody (MCL-1801): sc-69841

Mcl-1 Antibody (MCL-1801) is a mouse monoclonal IgG1 antibody raised against recombinant full-length human Mcl-1, suitable for detecting Mcl-1 in human samples via western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Mcl-1, also called Myeloid cell leukemia-1, Mcl1, and Gm52627, is a crucial anti-apoptotic protein within the B-cell lymphoma 2 (Bcl-2) family, distinguished by its unique structure that includes three Bcl-2 homology (BH) domains, BH1, BH2, and BH3, as well as an extended N-terminal region that is less conserved among family members. This structural configuration is significant because the BH domains are essential for Mcl-1′s ability to bind and inhibit pro-apoptotic proteins like Bak and Bax, preventing mitochondrial outer membrane permeabilization and the initiation of apoptosis. The flexible N-terminal region contains multiple regulatory elements and phosphorylation sites that control Mcl-1′s stability, interactions, and rapid turnover, allowing cells to swiftly adjust Mcl-1 levels in response to stress or survival signals. Understanding the structure of Mcl-1 is crucial because alterations in its structural domains can affect its anti-apoptotic function and are implicated in the development of various cancers, where overexpression or stabilization of Mcl-1 leads to enhanced cell survival and resistance to chemotherapy. Anti-Mcl-1 antibody (MCL-1801) thus serves as an invaluable tool for researchers studying the structural biology of apoptosis regulation and the role of Mcl-1 in human diseases.

The preferred Mcl-1 antibody is Mcl-1 Antibody (22): sc-12756.