Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LOX CRISPR/Cas9 KO Plasmid (m): sc-421456
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOX CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LOX Antibody (F-8): sc-373995
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOX CRISPR/Cas9 KO Plasmid (m) | sc-421456 | 20 µg | $397.00 |
Overview
Mouse Lox encodes lysyl oxidase (LOX), a secreted copper-dependent amine oxidase that catalyzes covalent crosslinking of collagen and elastin, thereby controlling extracellular matrix maturation and biomechanical tissue integrity. LOX activity shapes ECM remodeling, regulates cell–matrix adhesion and migration, and influences signaling circuits linked to fibrosis and mechanotransduction, including integrin/FAK and downstream MAPK pathways. Dysregulated LOX expression or activity is associated with aberrant matrix stiffening and remodeling observed in fibrotic disorders, vascular pathology, and tumor-associated stromal changes, making Lox a useful node for studying microenvironment-driven phenotypes.
LOX CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lox gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lox together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lox open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOX protein expression.
This CRISPR knockout system enables efficient generation of Lox-deficient cell models for investigation of LOX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lox exon(s) critical for LOX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lox genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOX CRISPR/Cas9 KO Plasmid (m) and LOX CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lox locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOX HDR Plasmid (m) and LOX HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lox homology arms to support homology-directed repair at defined Lox target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.