Date published: 2025-9-7
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LDH Antibody (H-10) - Western Blotting - Image 377957
LDH 抗体 (H-10): sc-133123. MCF7 (A), U-2 OS (B), U-87 MG (C) 和 A-673 (D) 全细胞裂解液中 LDH 表达的 Western 印迹分析.
LDH 抗体 (H-10): sc-133123. MCF7 (A), U-2 OS (B), U-87 MG (C) 和 A-673 (D) 全细胞裂解液中 LDH 表达的 Western 印迹分析.

LDH 抗体 (H-10): sc-133123

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说明书
  • LDH 抗体 H-10 是小鼠单克隆 IgG2a κ，LDH抗体, 在59篇文献中引用，规格为200 µg/ml
  • 针对氨基酸173-332，在LDH-A的human起源的C-terminus处进行映射
  • LDH 抗体 (H-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 LDH-A, LDH-B, LDH-C, LDH-A-like 6A and LDH-A-like 6B
  • 抗LDH抗体（H-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LDH (H-10): sc-133123的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 LDH 抗体 (H-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LDH 抗体 (H-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加LDH抗体，包括LDH-A, LDH-B, LDH-C和LDH-D

LDH抗体（H-10）是一种IgG2a κ小鼠单克隆LDH抗体（也称为LHDA/B/C抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（石蜡）和酶联免疫吸附测定法检测小鼠、大鼠和人类来源的LDH蛋白。LDH抗体（H-10）既有非偶联形式的抗LDH抗体，也有多种偶联形式的抗LDH抗体，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。乳酸脱氢酶家族（LDH）催化厌氧糖酵解的最后一步，即L-乳酸和NAD转化为丙酮酸和NADH。LDH家族由三个成员组成，LDH-A、LDH-B和LDH-C，它们都形成由四个亚基组成的四聚体。然而，每个家族成员都显示出特定的组织分布模式，LDH-A和LDH-B在多种组织中占主导地位，特别是在肌肉中的LDH-A和心脏中的LDH-B，而LDH-C的表达仅限于睾丸和精子。LDH是生殖细胞肿瘤的有力标志物。编码人类LDH-A和LDH-C的基因位于11号染色体上，而人类LDH-B基因位于12号染色体上。LDH-A基因缺陷与运动性肌红蛋白尿有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LDH 抗体 (H-10) 参考文献:

  1. [肿瘤标志物在生殖细胞瘤治疗中的作用]。  |  Niwakawa, M. and Tobisu, K. 2001. Gan To Kagaku Ryoho. 28: 1159-65. PMID: 11525037
  2. 大肠杆菌表达的人类睾丸特异性乳酸脱氢酶的特性。  |  LeVan, KM. and Goldberg, E. 1991. Biochem J. 273 (Pt 3): 587-92. PMID: 1996957
  3. 决定人类精子特异性乳酸脱氢酶 LDHC 的基因座与 LDHA 同源。  |  Edwards, YH., et al. 1987. Dev Genet. 8: 219-32. PMID: 2844458
  4. LDH-A 负向调节结直肠癌中的 dMMR  |  Zhang, Y., et al. 2021. Cancer Sci. 112: 3050-3063. PMID: 34110068
  5. LDH 在癌症中的多重作用  |  Claps, G., et al. 2022. Nat Rev Clin Oncol. 19: 749-762. PMID: 36207413
  6. 以 LDH 为靶点的癌症免疫疗法的原理。  |  Miholjcic, TBS., et al. 2023. Eur J Cancer. 181: 166-178. PMID: 36657325
  7. 乳酸脱氢酶（LDH）同工酶谱可优化T细胞糖酵解和分化控制。  |  Chen, X., et al. 2023. Sci Adv. 9: eadd9554. PMID: 36961904
  8. 基于 LDH 的 "智能 "腐蚀传感和保护膜  |  Zhao, X., et al. 2023. Materials (Basel). 16: PMID: 37176365
  9. LDH与黑色素瘤  |  Finck, SJ., et al. 1983. Cancer. 51: 840-3. PMID: 6821850
  10. 乳酸脱氢酶 M-亚基缺乏症：临床特征, 代谢背景和遗传异质性。  |  Kanno, T. and Maekawa, M. 1995. Muscle Nerve Suppl. 3: S54-60. PMID: 7603529
  11. 乳酸脱氢酶的分离方法和临床意义。  |  Kopperschläger, G. and Kirchberger, J. 1996. J Chromatogr B Biomed Appl. 684: 25-49. PMID: 8906464
  12. 超嗜热细菌 thermotoga maritima 的乳酸脱氢酶：2.1 A 分辨率的晶体结构揭示了蛋白质内在稳定的策略。  |  Auerbach, G., et al. 1998. Structure. 6: 769-81. PMID: 9655830

订购信息

产品名称产品编号规格价格数量收藏夹

LDH 抗体 (H-10)

sc-133123
200 µg/ml
$316.00

LDH (H-10): m-IgGκ BP-HRP 套装

sc-521240
200 µg Ab, 40 µg BP
$354.00

LDH (H-10): m-IgG2a BP-HRP 套装

sc-546090
200 µg Ab; 10 µg BP
$354.00

LDH 抗体 (H-10) AC

sc-133123 AC
500 µg/ml, 25% agarose
$416.00

LDH 抗体 (H-10) HRP

sc-133123 HRP
200 µg/ml
$316.00

LDH 抗体 (H-10) FITC

sc-133123 FITC
200 µg/ml
$330.00

LDH 抗体 (H-10) PE

sc-133123 PE
200 µg/ml
$343.00

LDH 抗体 (H-10) Alexa Fluor® 488

sc-133123 AF488
200 µg/ml
$357.00

LDH 抗体 (H-10) Alexa Fluor® 546

sc-133123 AF546
200 µg/ml
$357.00

LDH 抗体 (H-10) Alexa Fluor® 594

sc-133123 AF594
200 µg/ml
$357.00

LDH 抗体 (H-10) Alexa Fluor® 647

sc-133123 AF647
200 µg/ml
$357.00

LDH 抗体 (H-10) Alexa Fluor® 680

sc-133123 AF680
200 µg/ml
$357.00

LDH 抗体 (H-10) Alexa Fluor® 790

sc-133123 AF790
200 µg/ml
$357.00

I used this antibody on frozen brain sections. The protein should be broadly expressed in the cortex but the staining is not showing any positive neuron, Can you please help me? I used 15 and 40 microm slices using antigen retrieval and permeabilization.

Asked by: Anonymous
Thank you for your question. Please get in touch with our technical support so we can address this issue and troubleshoot your experiment.
Answered by: Tech Support Europe
Date published: 2019-06-28

If I want to make sandwich immunoassay for human LDH using this antibody (H-10) as capturing (or detection), what antibody should I use as a counter part? Can you suggest antibody pairs for LDH sandwich assay?

Asked by: Teamj23
Thank you for your question. Unfortunately we do not support sandwich ELISA and have not tested these antibodies for that use. Any LDH antibody with a different epitope should be able to work for you, so sc-137243 might be a good choice as it is made to a different section of the protein.
Answered by: Tech Service
Date published: 2019-02-12

Will this antibody (H-10, sc-133123) detect bovine LDH-B in a heart extract on WB analysis?

Asked by: DrM1
Thank you for your question. This antibody hasn’t been tested in bovine samples. This monoclonal antibody was raised against amino acids 173-332 mapping at the C-terminus of LDH-A of human origin. This antigen shares 72% sequence identity with LDHB in Bos taurus (NP_776525) [and 93% with LDHA in bovine]. Based on this sequence analysis, the antibody may cross-react with these proteins in bovine samples.
Answered by: Technical Support
Date published: 2019-01-26

Can you recommend a positive control to use with LDH (H-10): sc-133123 monoclonal antibody for Western blot?

Asked by: jenni
We recommend MDA-MB-231 (sc-2232), Ca Ski (sc-364360) or ES-2 (sc-24674) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of LDH in these cell lines.
Answered by: Technical Support 7
Date published: 2021-12-21
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Rated 4 out of 5 by from The LDH antibody worked wellThe LDH antibody worked well in human tissues in paraffin
Date published: 2024-09-17
Rated 5 out of 5 by from Excellent in western blottingWestern blotting on CFBE cells (human bronchial cells) Dilution: 1:1000 Very specific signal at the expected molecular weight that decrease after treatment with siRNA oligo targeting UBC9.
Date published: 2024-03-07
Rated 5 out of 5 by from Excellent in western blotting as loading markerApplication: western blotting Dilution: 1:1000. Excellent and specific band at the expected molecular weight.
Date published: 2023-12-27
Rated 1 out of 5 by from Doesn't work in Leukemia CellsWe tested this antibody in two different Leukemia cells (K-562 and U-937) using the following protocol: Blocking in 5% no-fat milk for 1 hour. Anti-LDH (H-10) 1:500 Overnight. Goat anti-Mouse 1:2000 at room temperature for 1 hour. Sadly, no bands have been detected.
Date published: 2020-11-09
Rated 4 out of 5 by from Buon funzionamentoHo testato questo anticorpo inizialmente con una diluizione 1:500 tramite WB, su campioni sia umani che murini. Il segnale risultava molto forte perciò ho effettuato diluizioni intermedie fino ad arrivare anche a 1:5000, la quale ritengo sia quella più appropriata per apprezzare delle differenze.
Date published: 2017-09-19
Rated 5 out of 5 by from Excellent antibody for WBTne antibody works very well in WB. Antibody dilution 1:1000 in 2% BSA.
Date published: 2017-08-16
Rated 5 out of 5 by from really excellent antibodyIt is really an excellent antibody, a clear and clean band is detected around 34KDa.
Date published: 2017-05-04
Rated 5 out of 5 by from Excellent LDH antibodyLDH (H-10) is well cited for Western blot (WB) in literature. And gives a strong band at 35 kDa in positive control MDA-MB-231 Cell Lysate: sc-2232.
Date published: 2017-01-20
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LDH 抗体 (H-10) is rated 4.5 out of 5 by 12.
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