KNP-I Antibody (D-5): sc-514347
- KNP-I Antibody (D-5) is a mouse monoclonal IgG2a κ KNP-I antibody provided at 200 µg/ml
- raised against amino acids 7-268 mapping at the C-terminus of KNP-1 of human origin
- KNP-I Antibody (D-5) is recommended for detection of KNP-I of human origin, D10Jhu81e of mouse origin and the corresponding rat homolog by WB, IP, IF and ELISA
- Anti-KNP-I Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for KNP-I Antibody (D-5) for WB applications. These reagents are now offered in bundles with KNP-I Antibody (D-5) (see ordering information below).
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KNP-I Antibody (D-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects KNP-I protein of human origin, D10Jhu81e protein of mouse origin, and the corresponding rat homolog by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-KNP-I antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KNP-I protein, also known as ES1 protein homolog or protein GT335, is a highly conserved mitochondrial protein playing a crucial role in cellular metabolism and energy production. KNP-I presence in mitochondria is vital for regulating metabolic pathways essential for maintaining cellular homeostasis. C21orf33, the gene encoding KNP-I, is located on human chromosome 21, near a region associated with various genetic disorders, including Down syndrome, characterized by an extra copy of chromosome 21. C21orf33 gene product overexpression may contribute to Down syndrome pathology, highlighting KNP-I importance in both normal physiology and disease states. KNP-I shows abundant expression in heart and muscle tissues, underscoring KNP-I significance in tissues with high energy demands.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KNP-I Antibody (D-5) References:
- Expression of cystathionine beta-synthase, pyridoxal kinase, and ES1 protein homolog (mitochondrial precursor) in fetal Down syndrome brain. | Shin, JH., et al. 2004. Neurochem Int. 45: 73-9. PMID: 15082224
- Isolation of cDNA for a novel human protein KNP-I that is homologous to the E. coli SCRP-27A protein from the autoimmune polyglandular disease type I (APECED) region of chromosome 21q22.3. | Nagamine, K., et al. 1996. Biochem Biophys Res Commun. 225: 608-16. PMID: 8753807
- Isolation and characterization of GT335, a novel human gene conserved in Escherichia coli and mapping to 21q22.3. | Lafrenière, RG., et al. 1996. Genomics. 38: 264-72. PMID: 8975701
- Isolation of a human gene (HES1) with homology to an Escherichia coli and a zebrafish protein that maps to chromosome 21q22.3. | Scott, HS., et al. 1997. Hum Genet. 99: 616-23. PMID: 9150728
- Characterization of a novel gene, C21orf2, on human chromosome 21q22.3 and its exclusion as the APECED gene by mutation analysis. | Scott, HS., et al. 1998. Genomics. 47: 64-70. PMID: 9465297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KNP-I Antibody (D-5) | sc-514347 | 200 µg/ml | $322.00 | |||
KNP-I Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-526362 | 200 µg Ab; 10 µg BP | $361.00 | |||
KNP-I Antibody (D-5): m-IgG2a BP-HRP Bundle | sc-546606 | 200 µg Ab; 10 µg BP | $361.00 | |||
KNP-I Antibody (D-5) AC | sc-514347 AC | 500 µg/ml, 25% agarose | $424.00 | |||
KNP-I Antibody (D-5) HRP | sc-514347 HRP | 200 µg/ml | $322.00 | |||
KNP-I Antibody (D-5) FITC | sc-514347 FITC | 200 µg/ml | $336.00 | |||
KNP-I Antibody (D-5) PE | sc-514347 PE | 200 µg/ml | $349.00 | |||
KNP-I Antibody (D-5) Alexa Fluor® 488 | sc-514347 AF488 | 200 µg/ml | $364.00 | |||
KNP-I Antibody (D-5) Alexa Fluor® 546 | sc-514347 AF546 | 200 µg/ml | $364.00 | |||
KNP-I Antibody (D-5) Alexa Fluor® 594 | sc-514347 AF594 | 200 µg/ml | $364.00 | |||
KNP-I Antibody (D-5) Alexa Fluor® 647 | sc-514347 AF647 | 200 µg/ml | $364.00 | |||
KNP-I Antibody (D-5) Alexa Fluor® 680 | sc-514347 AF680 | 200 µg/ml | $364.00 | |||
KNP-I Antibody (D-5) Alexa Fluor® 790 | sc-514347 AF790 | 200 µg/ml | $364.00 |