Date published: 2025-9-6
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KLF15 Antibody (A-5) - Western Blotting - Image 401997
KLF15 抗体 (A-5) Alexa Fluor® 488: sc-271675 AF488. KLF15 在 SH-SY5Y (A) 和 K-562 (B) 全细胞裂解液及人肝组织提取物 (C) 中表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
KLF15 抗体 (A-5) Alexa Fluor® 488: sc-271675 AF488. KLF15 在 SH-SY5Y (A) 和 K-562 (B) 全细胞裂解液及人肝组织提取物 (C) 中表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

KLF15 抗体 (A-5): sc-271675

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说明书
  • KLF15 抗体 A-5 是小鼠单克隆 IgG2a κ，KLF15抗体, 在15篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的KLF15的N-terminus的氨基酸1-23之间
  • KLF15 抗体 (A-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 KLF15
  • 抗KLF15抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271675 X, 200 µg/0.1 ml)
  • 关于如何获取KLF15 (A-5): sc-271675的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是KLF15 Antibody (A-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与KLF15 Antibody (A-5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外KLF抗体，包括KLF12, KLF15, KLF16, KLF17, KLF3, KLF6, KLF7, KLF8和KLK6

KLF15抗体（A-5）是一种IgG2a κ小鼠单克隆KLF15抗体（也称为KKLF抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的KLF15蛋白质。KLF15抗体（A-5）既可以是非偶联的抗KLF15抗体形式，也可以是多种偶联形式的抗KLF15抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。KLF15、KLF6和KLF3是含Krüppel样锌指的转录因子。KLF15是一种富含肾脏的Krüppel样因子，是一种结合CLCNKA启动子的转录激活剂。KLF6（也称为Zf9或CPBP，即核心启动子结合蛋白）在肝星状细胞激活过程中迅速诱导，并反式激活由Collagen I启动子驱动的报告基因，这表明KLF6在组织损伤应答中发挥作用。KLF3可能在造血中发挥作用。KLF15是一种核蛋白，主要在肝脏、心脏、骨骼肌和肾脏组织中表达，但在淋巴组织或骨髓中未检测到。它是脂肪和肌肉组织中GLUT4的重要调节因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

KLF15 抗体 (A-5) 参考文献:

  1. 类克鲁珀因子 KLF15 可调节胰岛素敏感型葡萄糖转运体 GLUT4。  |  Gray, S., et al. 2002. J Biol Chem. 277: 34322-8. PMID: 12097321
  2. Kruppel 样因子 15 是一种锌指转录调节因子，可抑制视黄素和视黄素受体间结合蛋白启动子。  |  Otteson, DC., et al. 2004. Invest Ophthalmol Vis Sci. 45: 2522-30. PMID: 15277472
  3. Krüppel 样因子 15 在肝脏 PEPCK 基因表达中的作用  |  Teshigawara, K., et al. 2005. Biochem Biophys Res Commun. 327: 920-6. PMID: 15649433
  4. Krüppel样因子15（KLF15）在脂肪生成的转录调控中的作用  |  Mori, T., et al. 2005. J Biol Chem. 280: 12867-75. PMID: 15664998
  5. 转录抑制因子 KLF15 的锌指结构域与视黄素和 IRBP 启动子中的多个位点结合，包括 CRS-1 和富含 G 的抑制因子。  |  Otteson, DC., et al. 2005. BMC Mol Biol. 6: 15. PMID: 15963234

订购信息

产品名称产品编号规格价格数量收藏夹

KLF15 抗体 (A-5)

sc-271675
200 µg/ml
$316.00

KLF15 (A-5): m-IgG Fc BP-HRP 套装

sc-529239
200 µg Ab; 10 µg BP
$354.00

KLF15 (A-5): m-IgGκ BP-HRP 套装

sc-522020
200 µg Ab, 40 µg BP
$354.00

KLF15 (A-5): m-IgG2a BP-HRP 套装

sc-547283
200 µg Ab; 10 µg BP
$354.00

KLF15 抗体 (A-5) X

sc-271675 X
200 µg/0.1 ml
$316.00

KLF15 抗体 (A-5) AC

sc-271675 AC
500 µg/ml, 25% agarose
$416.00

KLF15 抗体 (A-5) HRP

sc-271675 HRP
200 µg/ml
$316.00

KLF15 抗体 (A-5) FITC

sc-271675 FITC
200 µg/ml
$330.00

KLF15 抗体 (A-5) PE

sc-271675 PE
200 µg/ml
$343.00

KLF15 抗体 (A-5) Alexa Fluor® 488

sc-271675 AF488
200 µg/ml
$357.00

KLF15 抗体 (A-5) Alexa Fluor® 546

sc-271675 AF546
200 µg/ml
$357.00

KLF15 抗体 (A-5) Alexa Fluor® 594

sc-271675 AF594
200 µg/ml
$357.00

KLF15 抗体 (A-5) Alexa Fluor® 647

sc-271675 AF647
200 µg/ml
$357.00

KLF15 抗体 (A-5) Alexa Fluor® 680

sc-271675 AF680
200 µg/ml
$357.00

KLF15 抗体 (A-5) Alexa Fluor® 790

sc-271675 AF790
200 µg/ml
$357.00

KLF15 (A-5) 中和勝肽

sc-271675 P
100 µg/0.5 ml
$68.00

Hello, Your KLF15 Antibody (A-5) X (Cat No. sc-271675 X) is the version upgraded fro ChIP. But, Can I use this antibody for other applications such as WB, IF, IHC, IP?

Asked by: Mariano
Thank you for your question. Yes, the "X" form of this primary antibody is provided 10x more concentrated (200 ug in 0.1 ml) than the conventional antibody (200 ug in 1 ml), (and in a buffer that does not contain gelatin). This antibody can be used in other research applications. We just recommend adjusting the recommended dilution ranges based on the different antibody concentration.
Answered by: Technical Support
Date published: 2019-05-29

What application is the blocking peptide sc-271675 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from いい抗体ですRAW264.7細胞のWhole-cell lysate（15 ug protein）を200倍希釈した抗体で検出
Date published: 2018-01-18
Rated 5 out of 5 by from One major band in WBWB on 10µg of total mouse heart protein extract. Gel 10%, single band detected at 1/1000 and 1/10000 for secondary Ab The MW is around 95KDa which suggest dimer?
Date published: 2017-10-21
Rated 5 out of 5 by from IHC-PNot validated for paraffin embedded tissue but with appropriate antigen retrieval, worked very well. 1:250 dilution
Date published: 2017-10-13
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of KLF15 expression in human liver and human heart tissue extracts.
Date published: 2017-02-03
Rated 5 out of 5 by from Used in WB and worked well in mouse samplesUsed in WB and worked well in mouse samples.
Date published: 2015-07-29
Rated 5 out of 5 by from Works great for human KLF15 transfected lysatesWorks great for human KLF15 transfected lysates. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from WB publishable data with human U2OS cellsWB publishable data with human U2OS cells - SCBT Publication Review
Date published: 2015-01-14
Rated 5 out of 5 by from Positive results in western blot using humanPositive results in western blot using human liver and human heart tissue extracts. -SCBT QC
Date published: 2015-01-12
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KLF15 抗体 (A-5) is rated 5.0 out of 5 by 9.
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