Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
IRP-2 Antibody (7H6) - Immunohistochemistry - Image 287068
IRP-2 抗体 (7H6): sc-33682. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
IRP-2 抗体 (7H6): sc-33682. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.

IRP-2 抗体 (7H6): sc-33682

4.6(11)
寫評論提問

说明书
  • IRP-2 抗体 7H6 是小鼠单克隆 IgG1 κ，IRP-2抗体, 在54篇文献中引用，规格为200 µg/ml
  • 针对全长重组IRP-2 (iron regulatory protein-2)，human起源
  • IRP-2 抗体 (7H6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 IRP-2
  • 抗IRP-2抗体（7H6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IRP-2 (7H6): sc-33682的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 IRP-2 抗体 (7H6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IRP-2 抗体 (7H6) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 IRP 抗体 包括 IRP-2

IRP-2抗体（7H6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人类IRP-2蛋白。IRP-2抗体（7H6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。IRP-2在铁代谢中起着至关重要的作用，而铁代谢对于维持细胞内环境平衡至关重要。IRP-2主要位于细胞质中，通过与铁响应元件（IRE）结合，对铁代谢起到关键调节作用。这种结合可以抑制翻译或稳定mRNA，从而控制转铁蛋白受体和铁蛋白等蛋白质的水平，它们分别是铁运输和储存所必需的。IRP-2能够响应铁含量波动和各种应激信号（包括一氧化氮和细胞因子），这凸显了其在细胞适应铁供应变化和氧化应激中的重要性。在研究中使用IRP-2 (7H6)抗体可以深入了解铁代谢的调节机制及其在各种生理和病理条件下的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IRP-2 抗体 (7H6) 参考文献:

  1. IRP2的S-亚硝基化通过泛素-蛋白酶体途径调节其稳定性。  |  Kim, S., et al. 2004. Mol Cell Biol. 24: 330-7. PMID: 14673166
  2. 硝普钠通过增加细胞内铁的含量，在 C178 处没有 S 亚硝基化的情况下促进 IRP2 降解。  |  Wang, J., et al. 2006. Mol Cell Biol. 26: 1948-54. PMID: 16479012
  3. 通过体外转录/翻译从全长人类 cDNA 中表达活性铁调节因子。  |  Hirling, H., et al. 1992. Nucleic Acids Res. 20: 33-9. PMID: 1738601
  4. IRP2 的铁依赖性降解需要其 C 端区域和 IRP 结构的完整性。  |  Wang, J., et al. 2008. BMC Mol Biol. 9: 15. PMID: 18226225
  5. 调节性 RNA 结合蛋白 IRE-BP, 乌头酶和异丙基丙二酸异构酶之间的同源性。  |  Hentze, MW. and Argos, P. 1991. Nucleic Acids Res. 19: 1739-40. PMID: 1903202
  6. 一种受调控的 RNA 结合蛋白也具有乌头酶活性。  |  Kaptain, S., et al. 1991. Proc Natl Acad Sci U S A. 88: 10109-13. PMID: 1946430
  7. 铁调控蛋白 2（IRP2）的致癌特性由其特定的 73 氨基酸插入物介导。  |  Maffettone, C., et al. 2010. PLoS One. 5: e10163. PMID: 20405006
  8. 克隆编码 RNA 调节蛋白--人类铁反应元件结合蛋白的 cDNA。  |  Rouault, TA., et al. 1990. Proc Natl Acad Sci U S A. 87: 7958-62. PMID: 2172968
  9. IRP2-Hif1α/Hif2α信号传导：从有氧糖酵解到氧化磷酸化代谢转换的新机制。  |  Zhu, L., et al. 2020. Acta Biochim Biophys Sin (Shanghai). 52: 1175-1177. PMID: 32813008
  10. 纳多洛尔通过靶向HOIL-1/IRP2通路减轻缺血性脑卒中大鼠脑细胞铁蛋白沉积。  |  Yang, XY., et al. 2024. CNS Neurol Disord Drug Targets.. PMID: 39252618
  11. 耶尔森菌染色体上的 irp2 基因：分布、表达、缺失及其对毒力的影响。  |  de Almeida, AM., et al. 1993. Microb Pathog. 14: 9-21. PMID: 8321119
  12. 氧化应激对真核生物铁代谢的影响。  |  Rouault, TA. and Klausner, RD. 1996. EXS. 77: 183-97. PMID: 8856975

订购信息

产品名称产品编号规格价格数量收藏夹

IRP-2 抗体 (7H6)

sc-33682
200 µg/ml
$316.00

IRP-2 (7H6): m-IgG Fc BP-HRP 套装

sc-526706
200 µg Ab; 10 µg BP
$354.00

IRP-2 (7H6): m-IgG1 BP-HRP 套装

sc-532079
200 µg Ab; 20 µg BP
$354.00

IRP-2 抗体 (7H6) AC

sc-33682 AC
500 µg/ml, 25% agarose
$416.00

IRP-2 抗体 (7H6) HRP

sc-33682 HRP
200 µg/ml
$316.00

IRP-2 抗体 (7H6) FITC

sc-33682 FITC
200 µg/ml
$330.00

IRP-2 抗体 (7H6) PE

sc-33682 PE
200 µg/ml
$343.00

IRP-2 抗体 (7H6) Alexa Fluor® 488

sc-33682 AF488
200 µg/ml
$357.00

IRP-2 抗体 (7H6) Alexa Fluor® 546

sc-33682 AF546
200 µg/ml
$357.00

IRP-2 抗体 (7H6) Alexa Fluor® 594

sc-33682 AF594
200 µg/ml
$357.00

IRP-2 抗体 (7H6) Alexa Fluor® 647

sc-33682 AF647
200 µg/ml
$357.00

IRP-2 抗体 (7H6) Alexa Fluor® 680

sc-33682 AF680
200 µg/ml
$357.00

IRP-2 抗体 (7H6) Alexa Fluor® 790

sc-33682 AF790
200 µg/ml
$357.00

Can this IRP-2 Antibody be used for an RNA pull-down assay? I need to extract the IRP2-mRNA complex from primary tissue of the mouse brain, and then do an RNA-seq and a mass spec.

Asked by: Carol86
Thank you for your question. This antibody is recommended for WB, IP, IHC and ELISA. It has not been tested for other uses. You can find previous publications with this antibody here: https://www.scbt.com/scbt/misc/citationSearch.jsp Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2018-11-30

Can sc-33682: IRP-2 (7H6) be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your inquiry. Yes, sc-33682: IRP-2 (7H6) is validated for use in IHC with formalin fixed, paraffin-embedded tissue sections. We recommend performing antigen retrieval with sodium citrate buffer, pH 6, and heat. The complete protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-10
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_33682, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 116ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from sc-33682endogenous IRP2 is detectable in Western, dilution with 1% BSA in T/TBS is better than 5% skim milk.
Date published: 2019-03-13
Rated 3 out of 5 by from needs optimizationMany non-specific bands in Western. 1% BSA is better than 5% skim milk throughout the Western procedures. IRP2 over expression can be easily detected.
Date published: 2018-03-22
Rated 5 out of 5 by from Strong WB and IHCGreat antibody for western blot and IHC staining !!
Date published: 2017-01-12
Rated 5 out of 5 by from Excellent antibody for Western blot!This antibody gives a strong signal in Western blot with minimal background!
Date published: 2016-12-16
Rated 5 out of 5 by from Really good and specific antibodyReally good and specific antibody. For western blot of HEK293T used at 1:100 dilution
Date published: 2015-10-08
Rated 5 out of 5 by from Really good and specific antibodyReally good and specific antibody. Used at 1:100 dilution for HEK293T cells
Date published: 2015-10-08
Rated 5 out of 5 by from StrongStrong, clean band in western blot analysis of IRP-2 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Publishable western blot data obtained usingPublishable western blot data obtained using 7H6 with U251 human astrocytoma cell lines. -SCBT Publication Review
Date published: 2015-04-01
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_33682, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT
IRP-2 抗体 (7H6) is rated 4.6 out of 5 by 11.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_33682, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。