Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
IRE1α Antibody (B-12) - Western Blotting - Image 353682
IRE1α (B-12): sc-390960. DU 145 (A) 和 Jurkat (B) 全细胞裂解液中 IRE1α 表达的 Western 印迹分析.
IRE1α (B-12): sc-390960. DU 145 (A) 和 Jurkat (B) 全细胞裂解液中 IRE1α 表达的 Western 印迹分析.

IRE1α 抗体 (B-12): sc-390960

4.4(27)
寫評論提問

说明书
  • IRE1 alpha 抗体 B-12 是小鼠单克隆 IgG1 κ，IRE1α抗体, 在68篇文献中引用，规格为200 µg/ml
  • 免疫human物种的IRE1α的氨基酸371-560
  • IRE1 alpha 抗体 (B-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 IRE1α
  • 抗IRE1 alpha抗体（B-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IRE1α (B-12): sc-390960的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是IRE1α Antibody (B-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IRE1α Antibody (B-12) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

IRE1抗体

IRE1α抗体（B-12）是一种IgG1 κ小鼠单克隆IRE1α抗体（也称为IRE1α抗体或ERN1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的IRE1α蛋白。IRE1α抗体（B-12）既可以是非偶联的抗IRE1α抗体形式，也可以是多种偶联形式的抗IRE1α抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。酵母和哺乳动物细胞的内质网（ER）内未折叠蛋白质的积累会激活未折叠蛋白质反应（UPR）途径，并导致参与蛋白质折叠的ER特异性基因的转录。UPR的激活需要ER跨膜激酶IRE1p（用于肌醇要求和ER到细胞核的信号传导蛋白）。IRE1α和IRE1β是酵母IRE1p的两个哺乳动物同源物。这些相关蛋白质定位于ER腔，并且都包含一个短的跨膜结构域，该结构域跨越ER膜和胞质Ser/Thr激酶结构域。IRE1的激活涉及蛋白质胞质部分的寡聚化和反式磷酸化，从而增强了其固有的激酶活性，进而刺激了UPR靶基因的转录。作为对压力的反应，ER哺乳动物细胞的传感器激活IRE1α和IRE1β，然后导致JNK（Jun N端激酶）的磷酸化和细胞MAP激酶途径的激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IRE1α 抗体 (B-12) 参考文献:

  1. 跨膜蛋白激酶 IRE1 将 ER 中的压力与 JNK 蛋白激酶的激活耦合在一起。  |  Urano, F., et al. 2000. Science. 287: 664-6. PMID: 10650002
  2. 代谢性ER应激传感器IRE1α抑制巨噬细胞的替代激活，并影响肥胖症患者的能量消耗。  |  Shan, B., et al. 2017. Nat Immunol. 18: 519-529. PMID: 28346409
  3. 小鼠脑外伤后，抑制中性粒细胞胞外陷阱的形成可通过STING/IRE1α途径减轻NLRP1依赖性神经元热解。  |  Cao, Y., et al. 2023. Front Immunol. 14: 1125759. PMID: 37143681
  4. 髓样细胞中HIF1α依赖性缺氧反应需要IRE1α。  |  Mawambo, G., et al. 2023. J Neuroinflammation. 20: 145. PMID: 37344842
  5. DDRGK1的缺失会损害脊柱骨骺发育不良中的IRE1α UFMylation。  |  Yang, X., et al. 2023. Int J Biol Sci. 19: 4709-4725. PMID: 37781516
  6. 靶向IRE1α可改变肿瘤微环境，增强前列腺癌的抗肿瘤免疫力。  |  Unal, B., et al. 2024. Nat Commun. 15: 8895. PMID: 39406723
  7. 编码内质网常驻蛋白的基因的转录诱导需要跨膜蛋白激酶。  |  Cox, JS., et al. 1993. Cell. 73: 1197-206. PMID: 8513503
  8. 通过 Ire1p 激酶与含有 Ada5p 的转录辅激活剂复合物的相互作用，内质网中的未折叠蛋白诱导了基因。  |  Welihinda, AA., et al. 1997. Proc Natl Acad Sci U S A. 94: 4289-94. PMID: 9113982
  9. 一种不同于哺乳动物折叠蛋白反应的途径调节非应激细胞中内质网伴侣蛋白的表达。  |  Brewer, JW., et al. 1997. EMBO J. 16: 7207-16. PMID: 9384597
  10. 在哺乳动物细胞中，从内质网到细胞核的应激反应途径需要一种新型双功能蛋白激酶/内切核酸酶（Ire1p）。  |  Tirasophon, W., et al. 1998. Genes Dev. 12: 1812-24. PMID: 9637683
  11. 哺乳动物 Ire1 的克隆揭示了 ER 应激反应的多样性。  |  Wang, XZ., et al. 1998. EMBO J. 17: 5708-17. PMID: 9755171
  12. 蛋白质的翻译和折叠是由内质网驻留激酶耦合完成的。  |  Harding, HP., et al. 1999. Nature. 397: 271-4. PMID: 9930704

订购信息

产品名称产品编号规格价格数量收藏夹

IRE1α 抗体 (B-12)

sc-390960
200 µg/ml
$316.00

IRE1α (B-12): m-IgGκ BP-HRP 套装

sc-523752
200 µg Ab, 40 µg BP
$354.00

IRE1α (B-12): m-IgG1 BP-HRP 套装

sc-533608
200 µg Ab; 20 µg BP
$354.00

IRE1α 抗体 (B-12) AC

sc-390960 AC
500 µg/ml, 25% agarose
$416.00

IRE1α 抗体 (B-12) HRP

sc-390960 HRP
200 µg/ml
$316.00

IRE1α 抗体 (B-12) FITC

sc-390960 FITC
200 µg/ml
$330.00

IRE1α 抗体 (B-12) PE

sc-390960 PE
200 µg/ml
$343.00

IRE1α 抗体 (B-12) Alexa Fluor® 488

sc-390960 AF488
200 µg/ml
$357.00

IRE1α 抗体 (B-12) Alexa Fluor® 546

sc-390960 AF546
200 µg/ml
$357.00

IRE1α 抗体 (B-12) Alexa Fluor® 594

sc-390960 AF594
200 µg/ml
$357.00

IRE1α 抗体 (B-12) Alexa Fluor® 647

sc-390960 AF647
200 µg/ml
$357.00

IRE1α 抗体 (B-12) Alexa Fluor® 680

sc-390960 AF680
200 µg/ml
$357.00

IRE1α 抗体 (B-12) Alexa Fluor® 790

sc-390960 AF790
200 µg/ml
$357.00

Hi, do you have any ref for the Flow use of this antibody? thank you

Asked by: Joy xn
Thank you for your question. We do not currently have references for Flow Cytometry.
Answered by: BlakeJ
Date published: 2024-08-02

Knocking-down Ire1, I validated this antibody for WB (strong band, disappears with siRNA) but not for IF (formaldehyde-fixed, strong signal, stays the same with siRNA). How did you validate this antibody in IF? Do you recommend an antigen retrieval step?

Asked by: ValLH
Thank you for your question. We have tested this antibody in immunofluorescence cell stainings with the standard protocol you can find on our website: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining If you are performing fixation with formaldehyde, antigen retrieval may be necessary. Recommended methods are listed in the protocol under the above link. Please contact our technical service team directly if the issue persists.
Answered by: Tech Support Europe
Date published: 2022-03-09

Can this antibody detect both phosphorylated and non-phosphorylated forms of IRE1-α？

Asked by: xiaotuichuchu
Thank you for your question. IRE1α Antibody (B-12): sc-390960 was aised against amino acids 371-560 of IRE1α of human origin. It recognizes both the phosphorylated and the non-phosphorylated IRE1α.
Answered by: Tech Support Europe
Date published: 2019-07-10

Does this antibody recognize Ire1 from Drosophila?

Asked by: FC 2018
Thank you for your question. IRE1α Antibody (B-12): sc-390960 is recommended for detection of IRE1α of mouse, rat and human origin by WB, IP, IF and ELISA. There is currently no data on the antibody’s reactivity in Drosophila. We have not received any customer feedback indicating reactivity in other species than mouse, rat, or human. Please contact our technical service for further information or to arrange for a free sample of the antibody to test whether it would be suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the exact epitope recognized by IRE1α Antibody (B-12): sc-390960?

Asked by: cjMara
IRE1α Antibody (B-12) is a mouse monoclonal raised against amino acids 371-560 of IRE1α of human origin. The exact epitope has not been determined.
Answered by: Technical Support 13
Date published: 2017-01-09
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_390960, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 110ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good Ab for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:200 for overnight @ 4oC.
Date published: 2024-12-10
Rated 5 out of 5 by from WBできれいにバンドが出たウエスタンブロットで使用しましたが、想定していた位置にきれいにシングルバンドが出ましたのお勧めできる商品でした。
Date published: 2024-05-28
Rated 3 out of 5 by from Average for WBWork OK in western blot analysis for rat renal tubular cells
Date published: 2023-09-13
Rated 4 out of 5 by from Good work!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-05-04
Rated 5 out of 5 by from IRE1 AntibodyIRE1 works perfectly for western blot giving a single and specific band. I highly recommend it!
Date published: 2023-01-13
Rated 4 out of 5 by from clean but weakWe got this as a sample. We tried it with mouse cortical neuron cultures lysate. The signal was ok but somewhat weak in our opinion.
Date published: 2018-11-17
Rated 5 out of 5 by from 抗体效果非常好，条带漂亮我一个月前购买了此产品，很高兴，使用了非常的好，有需要这个指标的童鞋们不要犹豫了，实验结果非常完美。
Date published: 2018-07-17
Rated 5 out of 5 by from Really nice band!We checked the antibody in MDCK cells (canine). We used the antibody in 1:1.000 dilution in bovine serum albumin (1% in TBST), and the secondary antibody in 1:10.0000 dilution. We got a great band, with the expected molecular weight.
Date published: 2018-04-09
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_27
  • loc_zh_CN, sid_390960, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 28ms
  • REVIEWS, PRODUCT
IRE1α 抗体 (B-12) is rated 4.4 out of 5 by 27.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_27
  • loc_zh_CN, sid_390960, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 118ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。