Date published: 2025-9-6
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IQGAP2 Antibody (A-4) - Immunohistochemistry - Image 156533
IQGAP2 抗体 (A-4): sc-17835. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色, 显示腺细胞的细胞骨架染色.
IQGAP2 抗体 (A-4): sc-17835. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色, 显示腺细胞的细胞骨架染色.

IQGAP2 抗体 (A-4): sc-17835

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说明书
  • IQGAP2 抗体 A-4 是小鼠单克隆 IgG1 κ，IQGAP2抗体, 在2篇文献中引用，规格为200 µg/ml
  • 与氨基酸519-727结合，映射到human起源的IQGAP2的内部区域
  • IQGAP2 抗体 (A-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 IQGAP2
  • 抗IQGAP2抗体（A-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IQGAP2 (A-4): sc-17835的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 IQGAP2 抗体 (A-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IQGAP2 抗体 (A-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 IQGAP 抗体 包括 IQGAP1, IQGAP2和IQGAP3

IQGAP2抗体（A-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的IQGAP2蛋白。抗IQGAP2抗体（A-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。IQGAP2在细胞信号传导和细胞骨架动力学中起着至关重要的作用，它就像一个支架，促进小GTP酶（如Cdc42和Rac1）与其他信号分子之间的相互作用。这些相互作用对于调节细胞粘附至关重要，而细胞粘附对于维持组织完整性和细胞通讯必不可少。IQGAP2与IQGAP1同源物包含多个保守结构域，包括RasGAP结构域和多个钙调蛋白结合结构域，这些结构域对于肌动蛋白结合和GTP酶调节非常重要。IQGAP2可与活性GTP结合和失活GDP结合的两种Cdc42结合，从而调节GTP酶活性，影响细胞迁移和增殖等多种细胞过程。IQGAP2与其他蛋白（如Rac1和钙调蛋白）的相互作用进一步凸显了其在协调复杂信号通路以控制细胞行为方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IQGAP2 抗体 (A-4) 参考文献:

  1. IQGAP1 和钙调素调节 E-cadherin 的功能。  |  Li, Z., et al. 1999. J Biol Chem. 274: 37885-92. PMID: 10608854
  2. 缺乏假定的 Cdc42 效应器 IQGAP1 的小鼠胃增生。  |  Li, S., et al. 2000. Mol Cell Biol. 20: 697-701. PMID: 10611248
  3. 在乳腺癌中，无论ER状态如何，IQGAP2表达的减少都会通过调节MEK-ERK和p38信号转导来促进EMT和抑制细胞凋亡。  |  Kumar, D., et al. 2021. Cell Death Dis. 12: 389. PMID: 33846302
  4. IQGAP2 是一个独立的预后因素，与 DLBCL 的免疫抑制有关。  |  Tang, T., et al. 2021. BMC Cancer. 21: 603. PMID: 34034707
  5. IQGAP2 的减少通过调节 MAPK/ERK 通路和细胞因子促进膀胱癌的发生  |  Song, F., et al. 2022. Int J Mol Sci. 23: PMID: 36362301
  6. EBV 转录激活因子 Rta 对 IQGAP2 的上调及其对 EBV 生命周期的影响。  |  Lin, KM., et al. 2023. J Virol. 97: e0054023. PMID: 37504571
  7. IQGAP2 调节血脑屏障免疫动态。  |  Katdare, KA., et al. 2024. bioRxiv.. PMID: 38645082
  8. N6-甲基腺苷修饰的ALDH9A1通过NPM1/IQGAP2/AKT信号通路调节透明细胞肾细胞癌的脂质积累和肿瘤进展。  |  Tan, D., et al. 2024. Cell Death Dis. 15: 520. PMID: 39039052
  9. 鉴定出一种与 RasGAP 相关蛋白 IQGAP1 序列高度相似的 Cdc42Hs 推定效应因子，以及一种与 IQGAP2 相似的 Cdc42Hs 结合伙伴。  |  McCallum, SJ., et al. 1996. J Biol Chem. 271: 21732-7. PMID: 8702968
  10. Ras GTPase-激活蛋白相关人类蛋白 IQGAP2 有一个潜在的肌动蛋白结合域，并与钙调素和 Rho 家族 GTPase 相互作用。  |  Brill, S., et al. 1996. Mol Cell Biol. 16: 4869-78. PMID: 8756646
  11. 小 GTPase Cdc42 与其 GTPase 激活蛋白和推定效应物之间相互作用的特征。比较 Cdc42 与 Cdc42 交互作用结构域结合的动力学特性。  |  Zhang, B., et al. 1997. J Biol Chem. 272: 21999-2007. PMID: 9268338
  12. IQGAP1 整合了 Ca2+/calmodulin 和 Cdc42 信号。  |  Ho, YD., et al. 1999. J Biol Chem. 274: 464-70. PMID: 9867866

订购信息

产品名称产品编号规格价格数量收藏夹

IQGAP2 抗体 (A-4)

sc-17835
200 µg/ml
$316.00

IQGAP2 (A-4): m-IgG Fc BP-HRP 套装

sc-528330
200 µg Ab; 10 µg BP
$354.00

IQGAP2 (A-4): m-IgGκ BP-HRP 套装

sc-520676
200 µg Ab, 40 µg BP
$354.00

IQGAP2 (A-4): m-IgG1 BP-HRP 套装

sc-542870
200 µg Ab; 20 µg BP
$354.00

IQGAP2 抗体 (A-4) AC

sc-17835 AC
500 µg/ml, 25% agarose
$416.00

IQGAP2 抗体 (A-4) HRP

sc-17835 HRP
200 µg/ml
$316.00

IQGAP2 抗体 (A-4) FITC

sc-17835 FITC
200 µg/ml
$330.00

IQGAP2 抗体 (A-4) PE

sc-17835 PE
200 µg/ml
$343.00

IQGAP2 抗体 (A-4) Alexa Fluor® 488

sc-17835 AF488
200 µg/ml
$357.00

IQGAP2 抗体 (A-4) Alexa Fluor® 546

sc-17835 AF546
200 µg/ml
$357.00

IQGAP2 抗体 (A-4) Alexa Fluor® 594

sc-17835 AF594
200 µg/ml
$357.00

IQGAP2 抗体 (A-4) Alexa Fluor® 647

sc-17835 AF647
200 µg/ml
$357.00

IQGAP2 抗体 (A-4) Alexa Fluor® 680

sc-17835 AF680
200 µg/ml
$357.00

IQGAP2 抗体 (A-4) Alexa Fluor® 790

sc-17835 AF790
200 µg/ml
$357.00

Hi I am using IQGAP1 and IQGAP2 antibodies for my studies. I would like to know their specificity. Will there be any cross interaction between these antibodies.

Asked by: anshu1997
Thank you for your question. Our IQGAP1 and IQGAP2 antibodies are specific for IQGAP1 or IQGAP2, and should not cross react with the other protein.
Answered by: Tech Service
Date published: 2020-06-01

I am using IQGAP2 (A-4): sc-17835 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good for WBWorks well in WB on multiple samples, (whole brain lysate, HEK293). When used at 1:1,000 in 1% non-fat dry milk a single major band of the correct size is observed with some very minor additional bands.
Date published: 2018-04-13
Rated 2 out of 5 by from Okay for western blotUsed 100 ug of tissue on a western blot and could see a band in the correct position, some non-specific bands make it slightly difficult to discern correct molecular weight other than that.
Date published: 2018-02-21
Rated 4 out of 5 by from Seemed good for western blottingI used mouse Hepa1-6 cell lysates with loading gradient to check this antibody in western blotting. Antibody was diluted 1000X and incubated at 4℃ overnight. The exposure results showed several bands from 130-170KD. But I didn’t use si specific genes to check whether this band was specific.
Date published: 2017-08-18
Rated 5 out of 5 by from completely workedOverexpressed GFP-IQ2 (human clone) in 293 cells was detected. endogenous IQ2 in 293 also. it did not cross overexpressed IQ1 and 3 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-03-23
Rated 5 out of 5 by from Great WB antibodyThis IQGAP2 monoclonal antibody works great in WB, producing strong bands with minimal background in NCI-H929 whole cell lysate and human liver tissue extract
Date published: 2017-01-31
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoskeletalProduced exceptional immunoperoxidase cytoskeletal staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Publishable data in WB using human AGSPublishable data in WB using human AGS, MKN45 cell lines. -SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from Strong bands seen in WB using Hep G2 wholeStrong bands seen in WB using Hep G2 whole cell lysate and mouse and rat liver extracts. -SCBT QC
Date published: 2015-04-26
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IQGAP2 抗体 (A-4) is rated 4.2 out of 5 by 9.
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