Date published: 2025-9-9

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IFN-γRα 抗体 (GIR-94): sc-12755

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说明书
  • IFN- gamma R alpha 抗体 GIR-94 是小鼠单克隆 IgG2b κ,IFN-γRα抗体, 在16篇文献中引用,规格为200 µg/ml
  • 针对纯化的IFN-γRα human 起源
  • IFN- gamma R alpha 抗体 (GIR-94) 推荐用于 WB, IP, IF 和 FCM,检测mouse, rat 和human 来源的 IFN-γRα
  • 抗IFN- gamma R alpha抗体(GIR-94)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IFN-γRα (GIR-94): sc-12755免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是IFN-γRα Antibody (GIR-94) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IFN-γRα Antibody (GIR-94) 打包提供(请参阅下面的订购信息)。

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関連項目

IFN-γRα抗体(GIR-94)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)检测小鼠、大鼠和人类来源的IFN-γRα蛋白。IFN-γRα单克隆抗体(GIR-94)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。γ干扰素受体在调节γ干扰素(一种参与免疫反应的关键细胞因子)的作用方面起着至关重要的作用。在γ干扰素结合后,受体会发生构象变化,激活相关的Janus激酶,导致转录因子(如Stat1)磷酸化。这一过程对于参与抗病毒反应、免疫调节和细胞增殖的基因的转录激活至关重要。该受体由两个亚基组成,即IFN-γRα和IFN-γRβ,后者对于信号转导和对γ干扰素的全面生物反应至关重要。值得注意的是,虽然IFN-γRα足以与配体结合,但激活IFN-γ诱导基因需要两个链同时存在,这凸显了它们在免疫信号通路中相互作用的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IFN-γRα 抗体 (GIR-94) 参考文献:

  1. 通过计算机辅助设计提高干扰素-γ 受体 1 与干扰素-γ 的亲和力。  |  Mikulecký, P., et al. 2013. Biomed Res Int. 2013: 752514. PMID: 24199198
  2. 干扰素γ受体 1-56C/T 基因多态性与结核病易感性之间的关系:一项荟萃分析。  |  Wang, W., et al. 2014. Chin Med J (Engl). 127: 3782-8. PMID: 25382336
  3. 人类干扰素-γ 受体。抗体的纯化, 表征和制备。  |  Novick, D., et al. 1987. J Biol Chem. 262: 8483-7. PMID: 2954953
  4. 人类干扰素-γ 受体的分子克隆和表达。  |  Aguet, M., et al. 1988. Cell. 55: 273-80. PMID: 2971451
  5. 麦加地区干扰素-γ受体-1基因启动子多态性与布鲁氏菌病的易感性。  |  Ismael, AB., et al. 2018. Afr Health Sci. 18: 1157-1165. PMID: 30766581
  6. I 型和 II 型干扰素受体。  |  Orchansky, P., et al. 1984. J Interferon Res. 4: 275-82. PMID: 6086780
  7. Jak 蛋白酪氨酸激酶的干扰素诱导核信号传导。  |  Silvennoinen, O., et al. 1993. Nature. 366: 583-5. PMID: 7504785
  8. 阐明干扰素-γ 作用的最新进展:分子生物学和生物功能。  |  Vilcek, J. and Oliveira, IC. 1994. Int Arch Allergy Immunol. 104: 311-6. PMID: 8038608
  9. 鉴定激活人类干扰素γ受体所需的辅助因子及其序列。  |  Soh, J., et al. 1994. Cell. 76: 793-802. PMID: 8124716
  10. 干扰素受体家族的一个新成员补充了鼠干扰素γ受体在人体细胞中的功能。  |  Hemmi, S., et al. 1994. Cell. 76: 803-10. PMID: 8124717
  11. Jak-STAT 通路和转录激活对 IFNs 及其他细胞外信号蛋白的反应。  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455
  12. 干扰素-γ 及其受体的分子细胞生物学。  |  Farrar, MA. and Schreiber, RD. 1993. Annu Rev Immunol. 11: 571-611. PMID: 8476573

订购信息

产品名称产品编号规格价格数量收藏夹

IFN-γRα 抗体 (GIR-94)

sc-12755
200 µg/ml
$316.00

IFN-γRα (GIR-94): m-IgG Fc BP-HRP 套装

sc-528256
200 µg Ab; 10 µg BP
$354.00

IFN-γRα (GIR-94): m-IgGκ BP-HRP 套装

sc-520587
200 µg Ab, 40 µg BP
$354.00

IFN-γRα (GIR-94): m-IgG2b BP-HRP 套装

sc-550013
200 µg Ab; 10 µg BP
$354.00

IFN-γRα 抗体 (GIR-94) AC

sc-12755 AC
500 µg/ml, 25% agarose
$416.00

IFN-γRα 抗体 (GIR-94) HRP

sc-12755 HRP
200 µg/ml
$316.00

IFN-γRα 抗体 (GIR-94) FITC

sc-12755 FITC
200 µg/ml
$330.00

IFN-γRα 抗体 (GIR-94) PE

sc-12755 PE
200 µg/ml
$343.00

IFN-γRα 抗体 (GIR-94) Alexa Fluor® 488

sc-12755 AF488
200 µg/ml
$357.00

IFN-γRα 抗体 (GIR-94) Alexa Fluor® 546

sc-12755 AF546
200 µg/ml
$357.00

IFN-γRα 抗体 (GIR-94) Alexa Fluor® 594

sc-12755 AF594
200 µg/ml
$357.00

IFN-γRα 抗体 (GIR-94) Alexa Fluor® 647

sc-12755 AF647
200 µg/ml
$357.00

IFN-γRα 抗体 (GIR-94) Alexa Fluor® 680

sc-12755 AF680
200 µg/ml
$357.00

IFN-γRα 抗体 (GIR-94) Alexa Fluor® 790

sc-12755 AF790
200 µg/ml
$357.00

Hi! Can sc-12755 be used for IHC staining? Also, is it membrane stained? Thanks in advance!

Asked by: XiaoJ
Thank you for your question. We have not yet tested this antibody, IFN-γRα Antibody (GIR-94): sc-12755, on formalin-fixed paraffin-embedded tissue sections, and so IHC(P) is not a recommended application at this time. However, we do recommend and warranty the antibody for IF, or immunofluorescent staining of fresh or frozen tissue sections or cells fixed in acetone or methanol. We do expect membrane staining for IFN-γRα. If you are looking for an antibody for IHC(P) in human tissues, we would recommend an alternative clone, IFN-γRα Antibody (D-3): sc-28363. A link to that product on our website is below. https://www.scbt.com/scbt/product/ifn-gammaralpha-antibody-d-3 (D-3): sc-28363.
Answered by: Technical Support
Date published: 2017-07-27

Can IFN-γRα (GIR-94): sc-12755 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IFN-γRα (GIR-94): sc-12755 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Hi, I am interested in using this antibody for Western Blot of mouse cells. However, I checked the same clone (GIR-94) from BD science, they said it is only used for human. Could you let me know if this antibody is suitable in detecting mouse IFNGR1?

Asked by: Mario
Thank you for your question. We have tested this mouse monoclonal, IFN-γRα Antibody (GIR-94): sc-12755, in mouse samples and have verified reactivity with mouse peripheral blood leukocytes in Flow Cytometry. Therefore, we do recommend this antibody for detection of mouse IFNGR1 by Western blotting as well.
Answered by: Technical Support
Date published: 2016-12-05
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Rated 3 out of 5 by from Buon funzionamento in IFHo provato questo anticorpo in IF (1:100) ottenendo un buon segnale.
Date published: 2022-03-10
Rated 5 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and shown a very strong signal, even at dilution 1:100
Date published: 2018-11-16
Rated 4 out of 5 by from Nice Results for WBReproducible Results for Western blot using lysates from different cell lines.
Date published: 2017-11-06
Rated 5 out of 5 by from worked well with mouse cells by flowWe got very good flow cytometry results in mouse ESCs and their differentiated fibroblasts with this antibody at 1:100
Date published: 2017-05-05
Rated 5 out of 5 by from published soonThis antibody worked well with human IFN-gamma R alpha precursor transfected 293T whole cell lysate by WB. The data will be published soon.
Date published: 2016-12-18
Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human dermal lesion tissue. -SCBT Publication Review
Date published: 2015-05-25
Rated 3 out of 5 by from Large robust bands with background seen usingLarge robust bands with background seen using Raji and AML-193 whole cell lysates. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from Great FCM data of mouse peripheral bloodGreat FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-05-27
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IFN-γRα 抗体 (GIR-94) is rated 4.4 out of 5 by 8.
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