Date published: 2025-9-7

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IFI-16 抗体 (1G7): sc-8023

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说明书
  • IFI-16 抗体 1G7 是小鼠单克隆 IgG1 κ,IFI-16抗体, 在104篇文献中引用,规格为200 µg/ml
  • 免疫human来源IFI-16位于N-terminus 的1-159氨基酸
  • IFI-16 抗体 (1G7) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA,检测human 来源的 IFI-16
  • 抗IFI-16抗体(1G7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IFI-16 (1G7): sc-8023免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 IFI-16 抗体 (1G7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IFI-16 抗体 (1G7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

IFI-16抗体(1G7)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)、流式细胞术(FCM)和酶联免疫吸附测定(ELISA)检测人类源性IFI-16蛋白。抗IFI-16抗体(1G7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。IFI-16在免疫反应中起着至关重要的作用,特别是在病毒DNA识别方面,这对于启动适当的免疫反应至关重要。IFI-16主要定位于细胞核,在那里,IFI-16作为转录调节因子,调节各种信号通路,包括由干扰素激活的信号通路。IFI-16检测和响应外来DNA的能力对于宿主抵御病毒感染至关重要,可触发I型干扰素和其他细胞因子的产生,从而增强免疫反应。IFI-16与免疫信号传导中其他蛋白质相互作用,例如STING(干扰素基因的刺激因子),进一步强调了IFI-16在先天免疫系统中的重要性。干扰素-γ可以在某些细胞类型中诱导IFI-16的表达,这突出了IFI-16在适应性免疫反应中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IFI-16 抗体 (1G7) 参考文献:

  1. IFI16通过STING-TBK1-NF-kB通路上调免疫微环境中的PD-L1,从而促进宫颈癌的进展。  |  Cai, H., et al. 2020. Biomed Pharmacother. 123: 109790. PMID: 31896065
  2. IFI16依赖的STING信号是HER2+乳腺癌抗HER2免疫反应的关键调节因子。  |  Ong, LT., et al. 2022. Proc Natl Acad Sci U S A. 119: e2201376119. PMID: 35878022
  3. IFI16通过多磷酸化相分离驱动先天性免疫信号传导。  |  Liu, D., et al. 2023. Nucleic Acids Res. 51: 6819-6840. PMID: 37283074
  4. IFI16在调控PAN凋亡中的作用及其对心脏病的影响  |  Chang, X., et al. 2024. Cell Death Discov. 10: 204. PMID: 38693141
  5. 人类髓系细胞核分化抗原基因是位于 1q 染色体上的至少两个相关干扰素诱导基因之一,这些基因在造血细胞中特异性表达。  |  Briggs, RC., et al. 1994. Blood. 83: 2153-62. PMID: 7512843
  6. IFI 16 基因编码一种核蛋白,它在人类髓性白血病细胞系中的表达受干扰素诱导。  |  Dawson, MJ. and Trapani, JA. 1995. J Cell Biochem. 57: 39-51. PMID: 7536752
  7. 一种脂多糖诱导的巨噬细胞基因(D3)是干扰素诱导基因簇的新成员,可选择性地在单核吞噬细胞中表达。  |  Tannenbaum, CS., et al. 1993. J Leukoc Biol. 53: 563-8. PMID: 7684766
  8. 作为转录调节器的干扰素诱导型 p202 蛋白:抑制 NF-kappa B, c-Fos 和 c-Jun 的活性。  |  Min, W., et al. 1996. Mol Cell Biol. 16: 359-68. PMID: 8524315
  9. 人类髓细胞核分化抗原基因启动子的特征。  |  Kao, WY., et al. 1996. Biochim Biophys Acta. 1308: 201-4. PMID: 8809111
  10. p202 抑制 E2F 介导的转录。  |  Choubey, D., et al. 1996. EMBO J. 15: 5668-78. PMID: 8896460
  11. p202 是一种干扰素诱导的转录调节剂,它能抑制 p53 肿瘤抑制蛋白的转录激活,而与 p202 结合的 p53 结合蛋白 1 片段则能克服这种抑制作用。  |  Datta, B., et al. 1996. J Biol Chem. 271: 27544-55. PMID: 8910340

订购信息

产品名称产品编号规格价格数量收藏夹

IFI-16 抗体 (1G7)

sc-8023
200 µg/ml
$316.00

IFI-16 (1G7): m-IgG Fc BP-HRP 套装

sc-528206
200 µg Ab; 10 µg BP
$354.00

IFI-16 (1G7): m-IgGκ BP-HRP 套装

sc-520528
200 µg Ab, 40 µg BP
$354.00

IFI-16 (1G7): m-IgG1 BP-HRP 套装

sc-542815
200 µg Ab; 20 µg BP
$354.00

IFI-16 抗体 (1G7) AC

sc-8023 AC
500 µg/ml, 25% agarose
$416.00

IFI-16 抗体 (1G7) HRP

sc-8023 HRP
200 µg/ml
$316.00

IFI-16 抗体 (1G7) FITC

sc-8023 FITC
200 µg/ml
$330.00

IFI-16 抗体 (1G7) PE

sc-8023 PE
200 µg/ml
$343.00

IFI-16 抗体 (1G7) Alexa Fluor® 488

sc-8023 AF488
200 µg/ml
$357.00

IFI-16 抗体 (1G7) Alexa Fluor® 546

sc-8023 AF546
200 µg/ml
$357.00

IFI-16 抗体 (1G7) Alexa Fluor® 594

sc-8023 AF594
200 µg/ml
$357.00

IFI-16 抗体 (1G7) Alexa Fluor® 647

sc-8023 AF647
200 µg/ml
$357.00

IFI-16 抗体 (1G7) Alexa Fluor® 680

sc-8023 AF680
200 µg/ml
$357.00

IFI-16 抗体 (1G7) Alexa Fluor® 790

sc-8023 AF790
200 µg/ml
$357.00

Could this antibody reactive with mouse sample?

Asked by: Anonymous
Thank you for your question. This antibody is recommended for human samples, not for mouse. Thank you.
Answered by: Jiajun Z
Date published: 2024-11-12

I use this antibody and the bands are super clear but I see 3 lower bands around 40-50 kDa and couldn't identify them. One of the wb pictures you have shown on the product sheet has the same bands so you might explain why the additional bands appear.

Asked by: mordor
Thank you for your question. This protein's entry in uniprot (accession Q16666) shows three shorter alternative isoforms. As they still contain the antibody's epitope sequence, they will be detected in western blots.
Answered by: Tech Support Europe
Date published: 2023-02-16

I work with mouse samples and need to do IHC. How can that work with this mouse monoclonal antibody?

Asked by: Cweed
We recommend using conjugated primary antibodies. This product, sc-8023, is available directly conjugated to FITC, AF488, and AF 647.
Answered by: TechService7
Date published: 2016-12-30
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Rated 5 out of 5 by from Great antibodyWe're using this antibody since many years, one of the best available on the market. Very clean signal in wb, detecting the three IFI16 isoforms
Date published: 2021-05-17
Rated 4 out of 5 by from RecommendedWorks well by Western blotting, detects multiple forms of endogenous IFI16.
Date published: 2020-05-20
Rated 5 out of 5 by from Very goodI ordered the IFI16 Ab (1G7) about one month ago. I have done a couple of repeated experiments, it really works well in western blot. We treated U373 cells with reagent at different concentrations, and collected total proteins after 24 h treatment (unpublished data).
Date published: 2018-04-26
Rated 5 out of 5 by from Great Antibody for IFI16. Works for WB, IFA and IPWorks for WB (1:1000), IFA (1:100) and IP (1:30). Human samples were used - B cell lymphoma (Cell lines), Endothelial cells (primary and cell lines).
Date published: 2017-11-07
Rated 5 out of 5 by from Good specificityWe obtained a right band in the right molecular weight 1:1000 dilution for WB.
Date published: 2017-05-12
Rated 5 out of 5 by from Good antibodyStrong band observed by WB using human tissue extract
Date published: 2017-04-19
Rated 5 out of 5 by from Excellent antibody!We have been using this antibody in many applications, IHC, IFL and ELISA, and always with excellent results. Highly recommended!
Date published: 2017-04-14
Rated 5 out of 5 by from works on macaque tissueDetects IFI-16 in paraffin embedded macaque retina tissue and by immunoblotting of retina lysates.
Date published: 2017-03-07
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IFI-16 抗体 (1G7) is rated 4.8 out of 5 by 19.
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