Id4抗体 (B-5) 推荐用于 mouse, rat 和 human 来源的Id4 WB, IP, IF 和 ELISA检测

Id4 抗体 (B-5): sc-365656

Id4抗体 (B-5) is rated 2.9 out of 5 by 7.
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说明书

    • 抗-Id4 抗体 B-5 是小鼠单克隆 IgG1 κ,Id4抗体, 在5篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列1-70位于human物种的Id4的N-terminus
    • 抗-Id4 抗体 (B-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Id4; 也和以下物种反应,包括: porcine
    • 抗-Id4 抗体 (B-5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-365656 X, 200 µg/0.1 ml)
    • 关于如何获取Id4 (B-5): sc-365656免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Id4 Antibody (B-5) for WB applications. This reagent is now offered in a bundle with Id4 Antibody (B-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can Id4 (B-5): sc-365656 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Id4 (B-5): sc-365656 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Id4 antibody (B-5) works great for IF staining I tried the Id4 antibody (B-5) on both 293T cells and human Id4 transfected 293T cells for the immunofluorescence staining at 1:300. It gave very specific nuclear staining on the endogenous Id4 in non-transfected 293T cells. For the human Id4 transfected 293T cells, the Id4 shows both cytoplasmic and nuclear staining pattern, which is consistent with the published data reporting Id4 cytoplasmic and nuclear localization in prostate cancer cells.
Date published: 2018-04-19
Rated 3 out of 5 by from Rat samples without band I'm sorry that the sample is only submitting the report until now. It's a busy time. Taking the time to try this antibody, found good effect in 293T cells: closed fast closed closed 30min beyotime liquid, first antibody is the product (1:700 dilution), second antibogy buy from abclonal anti mouse (1:30000 dilution), the exposure time is 60 seconds.The molecular weight is about 18KD.But there was no band on the rat BMSC cells, and that was the case for the two time. It is also necessary to continue to work hard.The picture is the result of 293T cell samples
Date published: 2018-01-04
Rated 1 out of 5 by from only background, no bands at expected size in WB In a large panel of cell lines expressing high levels of ID4 we didn't get a specific signal at the expected protein size of 18kDa, however the overall background was quite strong. We used the antibody in TBST, 5% BSA diluted 1:100.
Date published: 2017-11-18
Rated 2 out of 5 by from No good for WB Antibody specificity is not good. The main band is not at the expected site (~15Kd).
Date published: 2017-09-11
Rated 3 out of 5 by from Works in Embryonic Neural Tissue for IHC Works fine in embryonic neural tissue 1:200 O/N with antigen retrieval, a little dirty.
Date published: 2017-08-17
Rated 1 out of 5 by from it is not good at IHC we tested this antibody in mouse, pig, dog, cow tissue sample. ID4 expression in the tissues was shown in other papers using other antibody and transgenic animal. but, by this antibody, not detect the ID4. So i want refund
Date published: 2017-02-17
Rated 5 out of 5 by from Produced positive Western blot data of Id4 Produced positive Western blot data of Id4 expression in non-transfected and human Id4 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-05-17
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