HSPC300 Antibody (G-4): sc-390459
- HSPC300 Antibody (G-4) is a mouse monoclonal IgG2a κ HSPC300 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-75 representing full length HSPC300 of human origin
- HSPC300 Antibody (G-4) is recommended for detection of HSPC300 of human origin, 6720456B07Rik of mouse origin and the corresponding rat homolog by WB, IP, IF and ELISA
- Anti-HSPC300 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for HSPC300 Antibody (G-4) for WB applications. This reagent is now offered in a bundle with HSPC300 Antibody (G-4) (see ordering information below).
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HSPC300 Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects HSPC300 protein of human, mouse, and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HSPC300 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSPC300 protein, also known as probable protein BRICK1 or C3orf10, consists of 75 amino acids and is expressed as two isoforms, localizing to both cytoplasm and cytoskeleton. HSPC300 plays a crucial role in regulating cytoskeletal organization and actin polymerization, which are essential processes for maintaining cell shape, motility, and division. HSPC300 exists as homotrimers before being incorporated into the WAVE complex, a multi-protein assembly that includes five proteins and is vital for the activation of the Arp2/3 complex, responsible for actin nucleation in a Rac1-dependent manner. HSPC300 conservation across various species suggests similar functions in different organisms, highlighting its importance in cellular processes. In Drosophila, the WAVE/Arp2/3 pathway is implicated in nervous system development, with HSPC300 localizing to axons and potentially influencing axonogenesis and synaptic morphogenesis in motoneurons. In mice, absence of the WAVE complex has been linked to learning and memory deficits, indicating HSPC300 may also play a role in cognitive functions. Furthermore, genetic depletion of HSPC300 has been associated with cytoskeletal abnormalities and impaired cytokinesis, suggesting potential involvement in tumor suppression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HSPC300 Antibody (G-4) References:
- Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. | Eden, S., et al. 2002. Nature. 418: 790-3. PMID: 12181570
- Solid renal tumor severity in von Hippel Lindau disease is related to germline deletion length and location. | Maranchie, JK., et al. 2004. Hum Mutat. 23: 40-6. PMID: 14695531
- Purification and architecture of the ubiquitous Wave complex. | Gautreau, A., et al. 2004. Proc Natl Acad Sci U S A. 101: 4379-83. PMID: 15070726
- Arabidopsis BRICK1/HSPC300 is an essential WAVE-complex subunit that selectively stabilizes the Arp2/3 activator SCAR2. | Le, J., et al. 2006. Curr Biol. 16: 895-901. PMID: 16584883
- Loss of the actin regulator HSPC300 results in clear cell renal cell carcinoma protection in Von Hippel-Lindau patients. | Cascón, A., et al. 2007. Hum Mutat. 28: 613-21. PMID: 17311301
- The N-terminus of Dictyostelium Scar interacts with Abi and HSPC300 and is essential for proper regulation and function. | Caracino, D., et al. 2007. Mol Biol Cell. 18: 1609-20. PMID: 17314411
- HSPC300 and its role in neuronal connectivity. | Qurashi, A., et al. 2007. Neural Dev. 2: 18. PMID: 17894861
- Free Brick1 is a trimeric precursor in the assembly of a functional wave complex. | Derivery, E., et al. 2008. PLoS One. 3: e2462. PMID: 18560548
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPC300 Antibody (G-4) | sc-390459 | 200 µg/ml | $322.00 | |||
HSPC300 Antibody (G-4): m-IgG2a BP-HRP Bundle | sc-545962 | 200 µg Ab; 10 µg BP | $361.00 | |||
HSPC300 Antibody (G-4) AC | sc-390459 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HSPC300 Antibody (G-4) HRP | sc-390459 HRP | 200 µg/ml | $322.00 | |||
HSPC300 Antibody (G-4) FITC | sc-390459 FITC | 200 µg/ml | $336.00 | |||
HSPC300 Antibody (G-4) PE | sc-390459 PE | 200 µg/ml | $349.00 | |||
HSPC300 Antibody (G-4) Alexa Fluor® 488 | sc-390459 AF488 | 200 µg/ml | $364.00 | |||
HSPC300 Antibody (G-4) Alexa Fluor® 546 | sc-390459 AF546 | 200 µg/ml | $364.00 | |||
HSPC300 Antibody (G-4) Alexa Fluor® 594 | sc-390459 AF594 | 200 µg/ml | $364.00 | |||
HSPC300 Antibody (G-4) Alexa Fluor® 647 | sc-390459 AF647 | 200 µg/ml | $364.00 | |||
HSPC300 Antibody (G-4) Alexa Fluor® 680 | sc-390459 AF680 | 200 µg/ml | $364.00 | |||
HSPC300 Antibody (G-4) Alexa Fluor® 790 | sc-390459 AF790 | 200 µg/ml | $364.00 |