Date published: 2025-9-7

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HSP70 抗体 (N27F3-4): sc-66049

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说明书
  • HSP70 抗体 N27F3-4 是小鼠单克隆 IgG1, 在14篇文献中引用,提供 100 µg/ml
  • 免疫human物种的HSP 70的氨基酸403-640
  • 推荐用于检测broad mammalian species、Drosophila melanogaster, zebrafish, Caenorhabditis elegans和plants来源的HSP 70,由WB, IP, IF, IHC(P)和FCM提供; 还建议用于检测HSC 70
  • 看 HSC70/HSP70 (W27): sc-24,可查看有标记的 HSP70 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取HSP70 (N27F3-4): sc-66049免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 HSP70 抗体 (N27F3-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HSP70 抗体 (N27F3-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

HSP 70抗体(N27F3-4)是一种IgG1 κ小鼠单克隆HSP 70抗体(也称为HSP 70抗体或热休克蛋白A(Hsp70)抗体),它能够通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组织化学法(IHC(P))和流式细胞仪(FCM)检测多种哺乳动物、黑腹果蝇、斑马鱼、秀丽隐杆线虫和植物来源的HSP 70。HSP 70抗体(N27F3-4)可作为非偶联抗HSP 70抗体形式提供。HSP 70家族由四个高度保守的蛋白质组成:HSP 70、HSC 70、GRP 75和GRP 78。这些蛋白质具有多种作用:它们作为分子伴侣促进多蛋白复合物的组装,参与多肽跨细胞膜和向细胞核的易位,并有助于新生多肽链的正确折叠。除HSP 70外,该家族的所有成员都在灵长类细胞中组成型表达。HSP 70的表达在热应激反应中被强烈诱导。HSP 70和HSC 70在细胞质内质网和线粒体输入机制中发挥关键作用,并存在于哺乳动物细胞的细胞质和细胞核中。HSP 70和HSC 70都参与新生多肽链的伴侣作用和保护细胞免受错误折叠蛋白质的积累。GRP 78定位于内质网,在那里它接收导入的分泌蛋白并参与新生肽链的折叠和易位。GRP 75的表达仅限于线粒体基质,并有助于核和线粒体来源的新生多肽链的易位和折叠。GRP 75和GRP 78对热应激无反应,由葡萄糖剥夺诱导。有人推测,HSP 70家族的成员充当产生力的马达,依靠ATP的水解来进行它们的活动。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

HSP70 抗体 (N27F3-4)

sc-66049
100 µg/ml
$316.00

HSP70 (N27F3-4): m-IgG Fc BP-HRP 套装

sc-537183
100 µg Ab; 10 µg BP
$354.00

HSP70 (N27F3-4): m-IgGκ BP-HRP 套装

sc-534386
100 µg Ab; 40 µg BP
$354.00

HSP70 (N27F3-4): m-IgG1 BP-HRP 套装

sc-545059
100 µg Ab; 20 µg BP
$354.00

Can i use this antibody for Golden hamster(mesocricetus auratus) for detection of HSP 70 antibody

Asked by: Surya
Thank you for your question. HSP70 Antibody (N27F3-4): sc-66049 is recommended for detection of HSP 70 of broad mammalian species, including mesocricetus auratus, by WB, IP, IF, IHC(P) and FCM.
Answered by: Tech Support Europe
Date published: 2025-04-30

Can this antibody be used for mouse serum samples?

Asked by: eleni28
Yes, broad mammalian species have been validated.
Answered by: Tech Sales
Date published: 2021-07-09

What is the total volume provided of HSP 70 (N27F3-4): sc-66049 monoclonal antibody?

Asked by: Cweed
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 5 out of 5 by from Great for western blotWe used this antibody for western blots of C2C12 lysates and got very sharp, clear bands at the correct molecular weight.
Date published: 2017-12-05
Rated 5 out of 5 by from Produced publishable WB data on human and ratThis HSP 70 antibody produced half a dozen citations in the primary literature for WB in human and rat samples.
Date published: 2017-01-20
Rated 5 out of 5 by from Good specificity and signal strength forGood specificity and signal strength for WB in NIH/3T3, heat shock treated NIH/3T3 and HeLa and heat shock treated HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by from Published in Western blot in BC3 and BCBL1Published in Western blot in BC3 and BCBL1 PEL cells. -SCBT Publication Review
Date published: 2015-01-21
Rated 5 out of 5 by from Good data for WB with transfected HUVGood data for WB with transfected HUV-EC-C whole cell lysates. -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Worked well with nonWorked well with non-transfected 293T, transfected 293T and HeLa whole cell lysate by WB. -SCBT QC
Date published: 2013-05-25
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HSP70 抗体 (N27F3-4) is rated 5.0 out of 5 by 7.
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