Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSP 40 Double Nickase Plasmid (h): sc-402810-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 40 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSP 40 Double Nickase Plasmid (h) and HSP 40 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DNAJB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 40 Antibody (B-3): sc-398766
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 40 Double Nickase Plasmid (h) | sc-402810-NIC | 20 µg | $410.00 | |||
HSP 40 Double Nickase Plasmid (h2) | sc-402810-NIC-2 | 20 µg | $410.00 |
DNAJB1 encodes the human HSP40 co-chaperone that regulates HSP70 ATPase cycling and promotes protein folding, refolding, and triage of misfolded clients during proteotoxic stress. Through its J-domain–mediated stimulation of HSP70, HSP40 supports cytosolic proteostasis networks that intersect with the unfolded protein response, heat shock signaling, and ubiquitin–proteasome and autophagy-mediated quality control. DNAJB1 activity influences maturation and stability of diverse signaling proteins, linking chaperone capacity to cell-cycle control and stress-adaptive transcriptional programs. Altered chaperone function or dysregulated DNAJB1 expression has been associated with proteostasis imbalance and disease-relevant phenotypes, including neurodegeneration and oncogenic stress contexts, making it a useful node for mechanistic studies of protein homeostasis.
HSP 40 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DNAJB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DNAJB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DNAJB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DNAJB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.