Date published: 2025-9-6
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HSF1 Antibody (E-4) - Western Blotting - Image 353982
HSF1 抗体 (E-4): sc-17757. 对 MCF7 (A), MDA-MB-231 (B), A2058 (C) 和 K-562 (D) 全细胞裂解液中 HSF1 的表达进行 Western 印迹分析.
HSF1 抗体 (E-4): sc-17757. 对 MCF7 (A), MDA-MB-231 (B), A2058 (C) 和 K-562 (D) 全细胞裂解液中 HSF1 的表达进行 Western 印迹分析.

HSF1 抗体 (E-4): sc-17757

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说明书
  • HSF1 抗体 E-4 是小鼠单克隆 IgG1 κ，HSF1抗体, 在51篇文献中引用，规格为200 µg/ml
  • 免疫human物种的heat shock transcription factor 1 (HSF1)的氨基酸219-529
  • HSF1 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HSF1
  • 抗HSF1抗体（E-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-17757 X, 200 µg/0.1 ml)
  • 关于如何获取HSF1 (E-4): sc-17757的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 HSF1 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与HSF1 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 HSF 抗体 包括 HSF1, HSF2, HSF4和HSF2BP

HSF1抗体（E-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的HSF1。HSF1单克隆抗体（E-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。HSF1通过调节热休克蛋白的表达，在细胞应激反应中发挥着至关重要的作用，尤其是热应激和化学应激，有助于保护细胞免受损伤。这种调节对于维持细胞内环境稳定和促进细胞在不利条件下的存活至关重要。HSF1通常以单体形式存在，但激活后形成三聚体并转移到细胞核，与目标基因启动子区域中的热休克元件结合，促进转录。雌激素可上调HSF1，从而增强mRNA和蛋白质水平上的活性，进一步强调了其在细胞应激反应中的重要性，以及与应激和炎症相关的疾病中的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSF1 抗体 (E-4) 参考文献:

  1. 真核生物中热休克基因的转录激活。  |  Tanguay, RM. 1988. Biochem Cell Biol. 66: 584-93. PMID: 3048332
  2. HSF1蛋白在恶性转化中的作用。  |  Šimončík, O., et al. 2018. Klin Onkol. 31: 55-62. PMID: 31023025
  3. HSF1和伴侣网络在肿瘤微环境中的作用。  |  Grunberg, N., et al. 2020. Adv Exp Med Biol. 1243: 101-111. PMID: 32297214
  4. HSF1通过调节APOJ/STAT3介导的PD-L1表达，参与免疫治疗反应。  |  Cheng, H., et al. 2023. Cancer Biol Ther. 24: 1-9. PMID: 36482717
  5. 热休克因子1（HSF1）可增强c-MYC介导的转录，而与典型的热休克反应无关。  |  Xu, M., et al. 2023. Cell Rep. 42: 112557. PMID: 37224019
  6. 癌细胞对热休克诱导刺激的转录反应涉及强效 HSF1 结合的放大。  |  Dastidar, SG., et al. 2023. Nat Commun. 14: 7420. PMID: 37973875
  7. 热休克转录因子的雌激素依赖性表达：对子宫合成热休克蛋白的影响。  |  Yang, X., et al. 1995. J Steroid Biochem Mol Biol. 52: 415-9. PMID: 7748806
  8. 利用扫描力显微镜确定环状 DNA 复合物中热休克转录因子 2 的化学计量。  |  Wyman, C., et al. 1995. EMBO J. 14: 117-23. PMID: 7828583
  9. 热休克因子 2 在小鼠胚胎发育过程中的功能和调控  |  Rallu, M., et al. 1997. Proc Natl Acad Sci U S A. 94: 2392-7. PMID: 9122205
  10. 热休克反应和蛋白质降解：泛素-蛋白酶体途径对 HSF2 的调控。  |  Mathew, A., et al. 1998. Mol Cell Biol. 18: 5091-8. PMID: 9710593
  11. 蛋白酶体抑制会导致激活热休克因子家族的所有成员。  |  Kawazoe, Y., et al. 1998. Eur J Biochem. 255: 356-62. PMID: 9716376
  12. 糖原合成酶激酶 3beta 和细胞外信号调节激酶通过促进热休克后转录活性颗粒的消失，使热休克转录因子 1 失活。  |  He, B., et al. 1998. Mol Cell Biol. 18: 6624-33. PMID: 9774677

订购信息

产品名称产品编号规格价格数量收藏夹

HSF1 抗体 (E-4)

sc-17757
200 µg/ml
$316.00

HSF1 (E-4): m-IgGκ BP-HRP 套装

sc-551063
200 µg Ab; 40 µg BP
$354.00

HSF1 抗体 (E-4) X

sc-17757 X
200 µg/0.1 ml
$316.00

HSF1 抗体 (E-4) AC

sc-17757 AC
500 µg/ml, 25% agarose
$416.00

HSF1 抗体 (E-4) HRP

sc-17757 HRP
200 µg/ml
$316.00

HSF1 抗体 (E-4) FITC

sc-17757 FITC
200 µg/ml
$330.00

HSF1 抗体 (E-4) PE

sc-17757 PE
200 µg/ml
$343.00

HSF1 抗体 (E-4) Alexa Fluor® 488

sc-17757 AF488
200 µg/ml
$357.00

HSF1 抗体 (E-4) Alexa Fluor® 546

sc-17757 AF546
200 µg/ml
$357.00

HSF1 抗体 (E-4) Alexa Fluor® 594

sc-17757 AF594
200 µg/ml
$357.00

HSF1 抗体 (E-4) Alexa Fluor® 647

sc-17757 AF647
200 µg/ml
$357.00

HSF1 抗体 (E-4) Alexa Fluor® 680

sc-17757 AF680
200 µg/ml
$357.00

HSF1 抗体 (E-4) Alexa Fluor® 790

sc-17757 AF790
200 µg/ml
$357.00

Hello, I am a research technician at the University of Bristol, UK. I am interested in your HSF1 (E-4) antibody. Do you have any unconjugated version of this antibody? Can I have a free sample to try it to my cell line. Many thanks.

Asked by: Andriana
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

I used 3ug of HSF1 (E-4) antibody for doing ChIP, but did not get much enrichment. Is there any recommended concentration of HSF1 antibody for ChIP that I should have used?

Asked by: S_G_
Thank you for your inquiry. For the IP step in ChIP we recommend using 100–500 µg supernatant and 0.1-1 µl TransCruz reagent (0.2–2 µg antibody). The complete protocol for ChIP can be found in below link. If you have any further questions, please call our Technical Service team toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website. Best of luck with your research! https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: Technical Support 14
Date published: 2017-07-10

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-13

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-17757 PE), fluorescein (sc-17757 FITC), Alexa Fluor® 488 (sc-17757 AF488) or Alexa Fluor® 647 (sc-17757 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-13
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Rated 5 out of 5 by from works goodworks good for my sample. cell line over-expressed with HSF1. 1:1000, clear background. but not sure for detecting endogenous level.
Date published: 2022-01-25
Rated 1 out of 5 by from Not good for WB using human whole cell lysate.This antibody is not good for WB using human K562 whole cell lysate at 1:500 dilution.
Date published: 2021-08-05
Rated 2 out of 5 by from Not working for my samplesI’ve tried this antibody on sub cellular fractionated PBMCs Lysates at 1:100 dilution but the signal was very low
Date published: 2020-09-02
Rated 3 out of 5 by from works okayfaint signals were observed - samples were human cells
Date published: 2017-11-21
Rated 5 out of 5 by from specifc signaWe got specific bands at 90kda with diution 1:800 by WB.HepG2 cell lysates 60ug.I don't recommend loading than 80ug, we have tried this amout ,it cause very high background,also I recommend use the secondary antibody sc-516102.
Date published: 2017-09-16
Rated 5 out of 5 by from 功能很出色the antibody is very good，economical and practical
Date published: 2017-09-12
Rated 4 out of 5 by from acceptable antibody for immunofluorescenceDilution 1:200 for IF on PFA fixed U2OS cells gives week signal (compared to polyclonal rabbit Ab). 1:50 should be tested.
Date published: 2017-08-08
Rated 5 out of 5 by from specific binding target proteinI use HSF1antibody in our WB assay.The antibody is very full bind the target protein,specific and 1:500 is suitable but for the loading amount I recommend controled at 40 to 70 ug lysates.If more than this range,it may affect the specificity and singal intensity.
Date published: 2017-07-15
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HSF1 抗体 (E-4) is rated 4.5 out of 5 by 19.
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