Date published: 2025-9-11
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
HPV16 L1 Antibody (CAMVIR-1) - Western Blotting - Image 392288
HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 488: sc-47699 AF488. 直接荧光西部印迹分析 ME-180 全细胞裂解液中 HPV16 L1 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
HPV16 L1 Antibody (CAMVIR-1) - Western Blotting - Image 392288
HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 488: sc-47699 AF488. 直接荧光西部印迹分析 ME-180 全细胞裂解液中 HPV16 L1 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 488: sc-47699 AF488. 直接荧光西部印迹分析 ME-180 全细胞裂解液中 HPV16 L1 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

HPV16 L1 抗体 (CAMVIR-1): sc-47699

4.4(7)
寫評論提問

说明书
  • HPV16 L1 抗体 CAMVIR-1 是小鼠单克隆 IgG2a κ，HPV16 L1抗体, 在19篇文献中引用，规格为200 µg/ml
  • 针对氨基酸的 198-531 HPV16 L1 重组蛋白
  • HPV16 L1 抗体 (CAMVIR-1) 推荐用于WB, IP, IF 和 IHC(P)，检测HPV-16 来源的 HPV16 L1; may cross react with HPV33; 可能与 HPV33产生交联反应; 不会与 HPV6 or HPV11产生交联反应
  • 抗HPV16 L1抗体（CAMVIR-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HPV16 L1 (CAMVIR-1): sc-47699的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 HPV16 L1 抗体 (CAMVIR-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HPV16 L1 抗体 (CAMVIR-1) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
说明书与实验方案
研究信息

関連項目

此外 HPV 抗体包括 HPV11 E7, HPV16 E1, HPV16 E18, HPV16 E2, HPV16 E4, HPV16 E6, HPV16 E7, HPV16 L1, HPV16 L2 和 HPV18 E2

HPV16 L1抗体（CAMVIR-1）是一种鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测HPV-16来源的HPV16 L1蛋白。抗HPV16 L1抗体（CAMVIR-1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HPV16 L1蛋白在病毒生命周期中起着至关重要的作用，是病毒衣壳的主要结构成分，对于保护和将病毒基因组传递到宿主细胞中至关重要。L1蛋白的五聚体组装形成了一个稳定的壳，有助于病毒最初附着在宿主细胞表面，这是感染过程中的关键一步。L1的C端参与DNA结合和包装，凸显了其在病毒复制周期中的重要性。L1蛋白的表达仅限于感染上皮的上层，这对于了解包括宫颈癌在内的HPV相关疾病的发病机理具有重要意义。HPV16 L1能够体外包装无关质粒DNA并将其传递给真核细胞，这凸显了其在基因治疗应用中的潜力。L1的特定定位模式以及与次要衣壳蛋白L2的相互作用，在被称为前单核细胞白血病蛋白致癌域（POD）的点状核区域被发现，这为深入了解HPV感染的复杂动态和所涉及的细胞机制提供了线索。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HPV16 L1 抗体 (CAMVIR-1) 参考文献:

  1. 用人乳头瘤病毒 16 型 (HPV-16) 病毒样颗粒或 HPV-16 L1 基因对小鼠进行鼻腔免疫，可在阴道引流淋巴结中激发特异性细胞毒性 T 淋巴细胞。  |  Dupuy, C., et al. 1999. J Virol. 73: 9063-71. PMID: 10516012
  2. 人类乳头瘤病毒 16 型负调控 RNA 元与在转录后不同水平发挥作用的三种蛋白质相互作用。  |  Koffa, MD., et al. 2000. Proc Natl Acad Sci U S A. 97: 4677-82. PMID: 10781073
  3. 由人类乳头瘤病毒 16 的 L1 蛋白组装而成的病毒样小颗粒的结构。  |  Chen, XS., et al. 2000. Mol Cell. 5: 557-67. PMID: 10882140
  4. 人类乳头瘤病毒 16 型的主要帽状蛋白的九个 C 端氨基酸对于 DNA 结合和基因转移能力至关重要。  |  Touzé, A., et al. 2000. FEMS Microbiol Lett. 189: 121-7. PMID: 10913877
  5. 用表达人乳头瘤病毒 16 型 (HPV16) L1 病毒样颗粒 (VLP) 或从昆虫细胞中纯化的 HPV16 VLP 重组鼠伤寒沙门氏菌进行粘膜疫苗接种，可抑制表达 HPV16 的肿瘤细胞在小鼠体内的生长。  |  Revaz, V., et al. 2001. Virology. 279: 354-60. PMID: 11145916
  6. 人类乳头瘤病毒 16 型小囊膜蛋白 l2 N 端区域含有一个常见的中和表位，可与细胞表面结合并进入细胞质。  |  Kawana, Y., et al. 2001. J Virol. 75: 2331-6. PMID: 11160736
  7. 病毒基因组封装所需的乳头瘤病毒 l2 的 L1 交互结构域。  |  Okun, MM., et al. 2001. J Virol. 75: 4332-42. PMID: 11287582
  8. 预防人类乳头瘤病毒 16 型性传播感染的疫苗方案。  |  Kowalczyk, DW., et al. 2001. Vaccine. 19: 3583-90. PMID: 11348726
  9. HPV16 L1的多样性及其对疫苗接种引起的免疫力的潜在影响。  |  El Aliani, A., et al. 2020. Gene. 747: 144682. PMID: 32304786
  10. 不同地理区域中HPV16 L1和L2基因的变异情况。  |  Tsakogiannis, D., et al. 2022. Viruses. 15: PMID: 36680181
  11. 乳头瘤病毒次要囊膜蛋白 L2 可诱导主要囊膜蛋白 L1 和病毒转录/复制蛋白 E2 定位于 PML 致癌结构域。  |  Day, PM., et al. 1998. J Virol. 72: 142-50. PMID: 9420209
  12. 真实人类乳头瘤病毒 16 型的体外感染和限型抗体介导的中和。  |  White, WI., et al. 1998. J Virol. 72: 959-64. PMID: 9444988

订购信息

产品名称产品编号规格价格数量收藏夹

HPV16 L1 抗体 (CAMVIR-1)

sc-47699
200 µg/ml
$316.00

HPV16 L1 (CAMVIR-1): m-IgG Fc BP-HRP 套装

sc-525554
200 µg Ab; 10 µg BP
$354.00

HPV16 L1 (CAMVIR-1): m-IgG2a BP-HRP 套装

sc-546312
200 µg Ab; 10 µg BP
$354.00

HPV16 L1 抗体 (CAMVIR-1) AC

sc-47699 AC
500 µg/ml, 25% agarose
$416.00

HPV16 L1 抗体 (CAMVIR-1) HRP

sc-47699 HRP
200 µg/ml
$316.00

HPV16 L1 抗体 (CAMVIR-1) FITC

sc-47699 FITC
200 µg/ml
$330.00

HPV16 L1 抗体 (CAMVIR-1) PE

sc-47699 PE
200 µg/ml
$343.00

HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 488

sc-47699 AF488
200 µg/ml
$357.00

HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 546

sc-47699 AF546
200 µg/ml
$357.00

HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 594

sc-47699 AF594
200 µg/ml
$357.00

HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 647

sc-47699 AF647
200 µg/ml
$357.00

HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 680

sc-47699 AF680
200 µg/ml
$357.00

HPV16 L1 抗体 (CAMVIR-1) Alexa Fluor® 790

sc-47699 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with HPV16 L1 Antibody (CAMVIR-1): sc-47699

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-09
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_47699, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 110ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works well in WBWorked really well in western blot, checked for 1:5000
Date published: 2022-06-15
Rated 1 out of 5 by from Antibody with issues!The Western Blot shown in the data sheet of “HPV16 L1 (CAMVIR-1): sc-47699, shows unspecific bands only, which are known to be seen with this antibody (Shafti-Keramat et al., J Virol. 2003 Dec;77(24):13125-35. PubMed PMID:14645569). There is no L1 signal depicted in the image (the correct size of L1 is markedly higher). As reported by Shafti-Keramat et al. it is an HPV unrelated dominant cellular band of app. 43 kDa. Furthermore it is known that HeLa cells are HPV type 18 positive (Lane A in the WB of the data sheet). We used this antibody, which gave us strong signals in the immunohistochemistry, implying the presence of HPV L1 in the tissue. This, however, could not be confirmed since the signal was derived from the unspecific HPV unrelated protein as seen in the Western blots. I therefore have to caution against working with this antibody, particularly when doing immuno(cyto)histochemistry. Using it in WB could potentially work since you at least could distinguish L1 from the unspecific band due to their different MW.
Date published: 2017-01-26
Rated 5 out of 5 by from Great antibodyThis antibody will provide strong and clear signals in standard applications. It has good data to support its use in WB and IHC and has been published with more than ten times.
Date published: 2017-01-16
Rated 5 out of 5 by from Outstanding western!!!Western blot results are outstanding; strong signal and low background
Date published: 2017-01-06
Rated 5 out of 5 by from Good publishable data in IF in human sperm cellsGood publishable data in IF in human sperm cells. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Strong band in WB in HeLaStrong band in WB in HeLa, Ca Ski and ME-180 whole cell lysates. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Good IF staining in human oocyte samplesGood IF staining in human oocyte samples. -SCBT Publication Review
Date published: 2013-08-04
  • y_2025, m_8, d_26, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_47699, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
HPV16 L1 抗体 (CAMVIR-1) is rated 4.4 out of 5 by 7.
  • y_2025, m_8, d_26, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_47699, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 80ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。