Date published: 2026-10-19
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HP1γ Antibody (F-1) - Western Blotting - Image 305788
HP1γ 抗体 (F-1): sc-398562. HeLa (A) 和 K-562 (B) 核提取物以及 K-562 (C), HeLa (D) 和 MCF7 (E) 全细胞裂解液中 HP1γ 表达的 Western 印迹分析.
HP1γ 抗体 (F-1): sc-398562. HeLa (A) 和 K-562 (B) 核提取物以及 K-562 (C), HeLa (D) 和 MCF7 (E) 全细胞裂解液中 HP1γ 表达的 Western 印迹分析.

HP1γ 抗体 (F-1): sc-398562

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说明书
  • HP1 gamma 抗体 F-1 是小鼠单克隆 IgG2a κ，HP1γ抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-104位于human物种的HP1γ的N-terminus
  • HP1 gamma 抗体 (F-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HP1γ
  • 抗HP1 gamma抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 2a BP-HRP">m-IgG2a BP-HRP是HP1γ Antibody (F-1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与HP1γ Antibody (F-1) 打包提供（请参阅下面的订购信息）。
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関連項目

额外HP1抗体，包括HP1 α, HP1 β和HP1 γ

HP1γ抗体（F-1）是一种IgG2a κ型小鼠单克隆HP1 gamma抗体（也称为Chromobox 3抗体、Heterochromatin Protein 1 Homolog Gamma抗体、Chromobox Protein Homolog 3抗体、Modifier 2 Protein抗体、HP1Hs-Gamma抗体、HECH抗体、Heterochromatin Protein HP1 Gamma抗体、HP1 Gamma Homolog (Drosophila)抗体、Heterochromatin-Like Protein 1抗体、Chromobox Homolog 3抗体、HP1 Gamma Homolog抗体或HP1-GAMMA抗体），它可以通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附试验（ELISA）检测到源自小鼠、大鼠和人类的HP1 gamma蛋白。HP1γ抗体（F-1）既有非偶联型抗HP1 gamma抗体形式，也有多种偶联型抗HP1 gamma抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。染色质组装因子-1（CAF-1）是一个由三个多肽亚基（称为p150、p60和p48）组成的多亚基蛋白复合物。CAF-1是一种核小体组装因子，在DNA复制过程中将新合成的乙酰化组蛋白H3/H4沉积到新生的染色质中。CAF-1的p150亚基还支持异染色质的维持，这需要新组蛋白和异染色质蛋白的合成及其在DNA复制过程中的有序组装。异染色质的特征是密集卷曲的染色质，通常在S期晚期复制，基因密度低，含有大量相对不易被DNA修饰试剂接触的重复DNA。在S期晚期，p150直接与异染色质相关蛋白1（HP1α、HP1β和HP1γ）结合。当细胞准备进行有丝分裂时，CAF-1 p150和一些HP1逐渐从异染色质中解离，这与组蛋白H3的磷酸化同时发生。HP1蛋白在有丝分裂结束时与染色质重新结合，此时组蛋白H3被去磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HP1γ 抗体 (F-1) 参考文献:

  1. 哺乳动物细胞中 HP1 蛋白在细胞周期中的定位和磷酸化。  |  Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
  2. 小鼠细胞中的异染色质动力学：染色质组装因子1和HP1蛋白之间的相互作用。  |  Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
  3. 异染色质结构域的复制和维护  |  Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
  4. 鉴定异染色质蛋白1（HP1）作为Pim-1激酶的磷酸化靶标以及磷酸化对HP1(1)转录抑制功能的影响。  |  Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
  5. DNA 复制过程中染色质组装所需的体外人类细胞因子 CAF-I 的纯化和特征描述。  |  Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
  6. 染色质组装因子 I 的 p150 和 p60 亚基：新合成组蛋白与 DNA 复制之间的分子联系。  |  Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
  7. 通过 CAF-1 和乙酰化组蛋白 H3/H4 复合物组装核小体。  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152

订购信息

产品名称产品编号规格价格数量收藏夹

HP1γ 抗体 (F-1)

sc-398562
200 µg/ml
$322.00

HP1γ (F-1): m-IgG2a BP-HRP 套装

sc-545973
200 µg Ab; 10 µg BP
$361.00

HP1γ 抗体 (F-1) AC

sc-398562 AC
500 µg/ml, 25% agarose
$424.00

HP1γ 抗体 (F-1) HRP

sc-398562 HRP
200 µg/ml
$322.00

HP1γ 抗体 (F-1) FITC

sc-398562 FITC
200 µg/ml
$336.00

HP1γ 抗体 (F-1) PE

sc-398562 PE
200 µg/ml
$349.00

HP1γ 抗体 (F-1) Alexa Fluor® 488

sc-398562 AF488
200 µg/ml
$364.00

HP1γ 抗体 (F-1) Alexa Fluor® 546

sc-398562 AF546
200 µg/ml
$364.00

HP1γ 抗体 (F-1) Alexa Fluor® 594

sc-398562 AF594
200 µg/ml
$364.00

HP1γ 抗体 (F-1) Alexa Fluor® 647

sc-398562 AF647
200 µg/ml
$364.00

HP1γ 抗体 (F-1) Alexa Fluor® 680

sc-398562 AF680
200 µg/ml
$364.00

HP1γ 抗体 (F-1) Alexa Fluor® 790

sc-398562 AF790
200 µg/ml
$364.00

What epitope (amino acid sequence/region) is recognized by HP1γ (F-1): sc-398562, and has it been validated in KO cells?

Asked by: cduval
Thank you for contacting Santa Cruz Biotechnology. sc-398562 HP1γ (F-1) was raised against amino acids 1-104 mapping at the N-terminus of HP1γ of human origin. The antibody has not been validated for knockout.
Answered by: BlakeJ
Date published: 2026-08-03

What is the recommended fixation method for immunofluorescence staining with HP1γ (F-1): sc-398562 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by rdmed from worked for ARPE-19worked well for immune fluorescent staining with ARPE-19 and mouse retina
Date published: 2022-09-01
Rated 5 out of 5 by HP1user from worked well for IF in PFA fixed cellsI received the Ab as sample. Used at 1:100. Bright labeling confined to the nucleus as expected.
Date published: 2019-05-04
Rated 5 out of 5 by YZ12 from Great antibody for IFI recently received the trial size of this antibody. It worked great for IF in my mouse cell line. Will post the images later.
Date published: 2018-01-31
Rated 5 out of 5 by han9350 from excellentThis secondary antibody works well when we use 1:2000 dilution in WB, and our results shows a beautiful brand.
Date published: 2017-09-20
Rated 5 out of 5 by Abreporter from an excellent antibody for both WB and IFThis antibody generates a clean and strong signal at the expected molecular weight (1:500 dilution, 40 ug of HeLa lysate). It also works well for IF in formaldehyde-fixed HeLa cells (1:200 dilution).
Date published: 2017-08-03
Rated 4 out of 5 by DaisyChina from Great immunohistochemical nuclear stainingGreat immunohistochemical nuclear staining in mouse glioma paraffin section.
Date published: 2017-01-20
Rated 5 out of 5 by bearpan310 from Worked great for WB !Worked great for WB with my cell line! It worked with concentration at 1:1000.
Date published: 2016-12-01
Rated 5 out of 5 by JM from Great immunofluorescence nuclear stainingGreat immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-19
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HP1γ 抗体 (F-1) is rated 4.8 out of 5 by 9.
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