Date published: 2025-9-6
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HNF-1β Antibody (94.8) - Western Blotting - Image 53904
HNF-1β (94. 8): sc-130407. 人皮肤组织提取物中 HNF-1β 表达的 Western 印迹分析.
HNF-1β Antibody (94.8) - Western Blotting - Image 53904
HNF-1β (94. 8): sc-130407. 人皮肤组织提取物中 HNF-1β 表达的 Western 印迹分析.
HNF-1β (94. 8): sc-130407. 人皮肤组织提取物中 HNF-1β 表达的 Western 印迹分析.

HNF-1β 抗体 (94.8): sc-130407

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说明书
  • HNF-1 beta 抗体 94.8 是小鼠单克隆 IgG2a κ，HNF-1β抗体, 在5篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组HNF-1β
  • 推荐用于 human 来源的HNF-1β WB 和 IP检测
  • 关于如何获取HNF-1β (94.8): sc-130407的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是HNF-1β Antibody (94.8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与HNF-1β Antibody (94.8) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

附加HNF抗体，包括HNF-1, HNF-1 α, HNF-1 β, HNF-3 α, HNF-3 β, HNF-3 γ, HNF-4 α和HNF-6

HNF-1β抗体（94.8）是一种IgG2a κ小鼠单克隆HNF-1β抗体（也称为HNF1B抗体或HNF1同源盒B抗体），该抗体通过蛋白质印迹(WB)和免疫沉淀(IP)检测人源的HNF-1β蛋白。HNF-1β抗体（94.8）可作为非偶联的抗HNF-1β抗体使用。HNF-1（α和β）、HNF-3（α、β和γ）、HNF-4（α和γ）和HNF-6部分地组成了一个被称为肝细胞核因子家族的同源蛋白家族。各种HNF-1异构体在肝脏以及其他组织（如肾脏、小肠和胸腺）中调节基因的转录。HNF-3α、HNF-3β和HNF-3γ在成年肝脏中调节众多肝细胞基因的转录。HNF-3α和HNF-3β也被证明参与原肠胚形成事件，如体轴的形成。HNF-4α和HNF-4γ已被证明对早期胚胎发育很重要。HNF-4α在肝脏、肾脏、胰腺、小肠、睾丸和结肠中表达；而HNF-4γ则在这些组织中表达，但肝脏除外。已证明HNF-6与HNF-3β的启动子结合，这表明HNF-6在肠道内胚层上皮细胞分化中具有潜在作用。有证据表明，HNF-6还可能是至少22个其他肝细胞富集基因的转录激活因子，包括细胞色素P450 2C13和α-1抗胰蛋白酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HNF-1β 抗体 (94.8) 参考文献:

  1. 囊性肾病, 嗜铬肾细胞癌和 TCF2（HNF1 beta）突变。  |  Lebrun, G., et al. 2005. Nat Clin Pract Nephrol. 1: 115-9. PMID: 16932376
  2. 肝细胞核因子-1β突变导致新生儿糖尿病和宫内发育迟缓：支持 HNF-1beta 在人类胰腺发育中的关键作用。  |  Edghill, EL., et al. 2006. Diabet Med. 23: 1301-6. PMID: 17116179
  3. 卵巢透明细胞腺癌中肝细胞核因子-1beta的特异性表达及其在细胞学诊断中的应用。  |  Higashiguchi, A., et al. 2007. Cancer Sci. 98: 387-91. PMID: 17270029
  4. 新生儿胆汁淤积性黄疸是肝细胞核因子-1beta 基因（HNF-1beta）突变的首发症状。  |  Beckers, D., et al. 2007. J Pediatr. 150: 313-4. PMID: 17307554
  5. 肝细胞核因子-1beta（HNF-1beta）免疫细胞化学：卵巢透明细胞癌的标志物。  |  Kato, N., et al. 2007. Diagn Cytopathol. 35: 193-7. PMID: 17351940
  6. 通过替代 RNA 处理，可生成更强的转录激活剂或 HNF1 同源蛋白家族的显性抑制剂。  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
  7. 小鼠转录因子 HNF-3 基因家族：基因结构, cDNA 序列和 mRNA 分布。  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
  8. 人类肝细胞核因子 4 同工型由不同的基因编码，且表达量不同。  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
  9. 转录激活因子肝细胞核因子 6 可调节肝脏基因的表达。  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

订购信息

产品名称产品编号规格价格数量收藏夹

HNF-1β 抗体 (94.8)

sc-130407
100 µg/ml
$333.00

HNF-1β (94.8): m-IgG Fc BP-HRP 套装

sc-539823
100 µg Ab; 10 µg BP
$354.00

HNF-1β (94.8): m-IgG2a BP-HRP 套装

sc-546791
100 µg Ab; 10 µg BP
$354.00

Hi, I am wondering if you also provide this antibody in an X/more concentrated format.

Asked by: bnch
Thank you for your question. Please contact our technical service directly.
Answered by: Tech Support Europe
Date published: 2019-09-23

Can this antibody (sc-130407) be used for mouse tissue? Can this antibody be used for immunofluorecence staining on paraffin sections?

Asked by: Xiangmin
Thank you for your question. This antibody has not been tested for mouse use, and is not recommended or warrantied for this use. We have not yet tested sc-130407 HNF-1β (94.8) on IHC. As a result, IHC is not currently a recommended application for this antibody.
Answered by: Tech Service
Date published: 2019-09-19

Is this antibody suitable for immunohistochemistry on formalin fixed paraffin embedded human tissue?

Asked by: cnotis
Thank you for your question. We have not yet tested this antibody on formalin-fixed paraffin embedded tissue. As a result, IHC(P) is not currently a recommended application for this antibody.
Answered by: Technical Support
Date published: 2018-12-13

Did you have any information on the effectiveness of this antibody on mouse liver IHC? Lineage fate of ductular reactions in carcinogenesis ... (Simone Jörs JCI 2015)

Asked by: MohamedBOUSALEH
Thank you for your question. This antibody sc-130407 is recommended for the detection of HNF-1 of human origin by WB and IP. We would not expect any significant cross-reactivity with mouse species. If you require more information, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Could I use this antibody for staining human ovary tumors in IHC? and if I could, how dilute range is recomended?

Asked by: a0061410
Thank you for your question. We have not yet tested this antibody, HNF-1β (94.8): sc-130407, for immunohistochemistry so we do not know if the antibody is suitable for this application. In general, we recommend using a starting dilution of 1:50 for IHC testing with similar antibodies.
Answered by: Technical Support
Date published: 2018-10-18

I am using HNF-1β (94.8): sc-130407 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24

Hi, I was wondering why the band showed in the western blot using HNF-1β Antibody sc-130407 is at 50 kDa and not higher around 60 kDa where I would expect based on the protein size.

Asked by: frdf
Thank you for your question. This antibody, HNF-1β Antibody (94.8): sc-130407, detected a signal around 50 kDa in human skin tissue extract by Western blotting. According to Uniprot, (http://www.uniprot.org/uniprot/P35680#sequences), HNF-1β is known to have several isoforms with predicted molecular weights of 61, 58, 45 and 47 kDa.
Answered by: Technical Support
Date published: 2017-03-14
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Rated 5 out of 5 by from Good for multiple applicationsThis antibody has been used for western blot, immunoperoxidase staining, and flow cytometry in published research. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Single band seen in Western Blot with humanSingle band seen in Western Blot with human skin tissue extract -SCBT QC
Date published: 2014-08-24
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HNF-1β 抗体 (94.8) is rated 4.5 out of 5 by 2.
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