Date published: 2025-9-8
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HMGCR Antibody (C-1) - Western Blotting - Image 349956
HMGCR 抗体 (C-1): sc-271595. Western 印迹分析 J774. A1 全细胞裂解液中 HMGCR 的表达.
HMGCR 抗体 (C-1): sc-271595. Western 印迹分析 J774. A1 全细胞裂解液中 HMGCR 的表达.

HMGCR 抗体 (C-1): sc-271595

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说明书
  • HMGCR 抗体 C-1 是小鼠单克隆 IgG1 κ，HMGCR抗体, 在65篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列589-888位于human物种的HMGCR的C-terminus
  • HMGCR 抗体 (C-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HMGCR
  • 抗HMGCR抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 HMGCR 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HMGCR 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

HMGCR抗体（C-1）是一种IgG1 κ小鼠单克隆HMGCR抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的HMGCR蛋白。HMGCR抗体（C-1）既有非偶联抗HMGCR抗体形式，也有多种偶联形式的抗HMGCR抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。人体内的羟甲基戊二酰辅酶A还原酶（HMGCR）会限制肝脏组织中胆固醇的合成速率，胆固醇合成是细胞生长所必需的过程。HMGCR的磷酸化会使酶失活，这是通过由还原酶反应产物衍生的固醇和非固醇代谢物介导的负反馈机制发生的。HMGCR抑制剂（他汀类药物）通过降低血浆胆固醇发挥抗炎作用，并减少心血管事件的发生频率。此外，HMGCR催化的通路中的中间产物可调节信号转导蛋白（如Ras），这为HMGCR调节与新的化疗方法之间提供了可能的联系。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HMGCR 抗体 (C-1) 参考文献:

  1. 急性肾小管损伤期间的甲羟戊酸途径：选定的决定因素和后果。  |  Zager, RA., et al. 2002. Am J Pathol. 161: 681-92. PMID: 12163393
  2. 胆固醇生物合成的分子调控：对致癌的影响。  |  Singh, RP., et al. 2003. J Environ Pathol Toxicol Oncol. 22: 75-92. PMID: 14533871
  3. 3-羟基-3-甲基戊二酰辅酶 a 还原酶抑制剂对冠状动脉内皮功能的快速影响。  |  Wassmann, S., et al. 2003. Circ Res. 93: e98-103. PMID: 14551237
  4. HMG-CoA 还原酶抑制剂可减少末端补体因子 C5b-9 对人血管平滑肌细胞的促炎性激活。  |  Viedt, C., et al. 2003. Basic Res Cardiol. 98: 353-61. PMID: 14556080
  5. 人类 3-羟基-3-甲基戊二酰辅酶 A 还原酶。负责催化活性和固醇调节降解的保守结构域。  |  Luskey, KL. and Stevens, B. 1985. J Biol Chem. 260: 10271-7. PMID: 2991281
  6. 喂食通过 mTORC1-USP20-HMGCR 轴诱导胆固醇的生物合成。  |  Lu, XY., et al. 2020. Nature. 588: 479-484. PMID: 33177714
  7. AMPK通过HMGCR/p38信号通路下调调节性T细胞中的PD-1，从而促进抗肿瘤免疫。  |  Pokhrel, RH., et al. 2021. Mol Cancer. 20: 133. PMID: 34649584
  8. HMGCR突变引起的肢体束带肌病和可由甲基戊二酸内酯治疗的他汀类药物肌病。  |  Yogev, Y., et al. 2023. Proc Natl Acad Sci U S A. 120: e2217831120. PMID: 36745799
  9. HMGCR抑制与类风湿性关节炎的关系：孟德尔随机化和共定位研究。  |  Ma, L., et al. 2023. Front Endocrinol (Lausanne). 14: 1272167. PMID: 38047111
  10. BRCC36通过去泛素化HMGCR来调节铁死亡和热死亡之间的相互作用。  |  Wang, H., et al. 2024. Adv Sci (Weinh). 11: e2304263. PMID: 38178583
  11. 通过Hedgehog信号抑制HMGCR抑制肝细胞癌的干细胞性和转移。  |  Zhang, Z., et al. 2024. Genes Dis. 11: 101285. PMID: 39022130
  12. 转基因小鼠大脑中 HMGCR 启动子-CAT 融合基因的细胞表达：少突胶质细胞发育调控的证据。  |  Duhamel-Clerin, E., et al. 1994. Glia. 11: 35-46. PMID: 8070893

订购信息

产品名称产品编号规格价格数量收藏夹

HMGCR 抗体 (C-1)

sc-271595
200 µg/ml
$316.00

HMGCR (C-1): m-IgG Fc BP-HRP 套装

sc-527261
200 µg Ab; 10 µg BP
$354.00

HMGCR (C-1): m-IgG1 BP-HRP 套装

sc-532634
200 µg Ab; 20 µg BP
$354.00

HMGCR 抗体 (C-1) AC

sc-271595 AC
500 µg/ml, 25% agarose
$416.00

HMGCR 抗体 (C-1) HRP

sc-271595 HRP
200 µg/ml
$316.00

HMGCR 抗体 (C-1) FITC

sc-271595 FITC
200 µg/ml
$330.00

HMGCR 抗体 (C-1) PE

sc-271595 PE
200 µg/ml
$343.00

HMGCR 抗体 (C-1) Alexa Fluor® 488

sc-271595 AF488
200 µg/ml
$357.00

HMGCR 抗体 (C-1) Alexa Fluor® 546

sc-271595 AF546
200 µg/ml
$357.00

HMGCR 抗体 (C-1) Alexa Fluor® 594

sc-271595 AF594
200 µg/ml
$357.00

HMGCR 抗体 (C-1) Alexa Fluor® 647

sc-271595 AF647
200 µg/ml
$357.00

HMGCR 抗体 (C-1) Alexa Fluor® 680

sc-271595 AF680
200 µg/ml
$357.00

HMGCR 抗体 (C-1) Alexa Fluor® 790

sc-271595 AF790
200 µg/ml
$357.00

Hello, I would like to use this antibody for flow cytometry. Can you share result of validating fluorescently-conjugated version in flow cytometry or an article that presents data from flow cytometry? I am looking forward to your reply, Best,  

Asked by: GosiaGarstka
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-01-25

这个抗体体积是多少，HMGCR 抗体 (C-1): sc-271595

Asked by: wwjdudu
Thank you for your question. The volume of this product is 1ml. 感谢提问。 这个产品体积是1ml。
Answered by: Ling Chen
Date published: 2022-08-24

Does this antibody recognize HMGCR produced by zebrafish? Are there any studies confirming this interaction?

Asked by: mateo
Thank you for your question. We are not aware of any publications citing use of this antibody, HMGCR (C-1): sc-271595, in zebrafish. The antibody is raised against amino acids 589-888 mapping at the C-terminus of HMGCR of human origin. If you can provide the accession number or protein sequence for the zebrafish protein we could perform sequence analysis to try and predict if the antibody may cross-react with this species. Please contact our Technical Service Department by calling us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-10-09

When would it be better to use sc-271595 AC instead of sc-271595?

Asked by: AbPolly
Thank you for your question. If the only application you intend to use this HMGCR antibody for is IP, then the agarose conjugated form sc-271595 AC is best. If multiple applications are required, then the unconjugated form sc-271595 is best.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from qualche aspecificoBuon funzionamento ma qualche banda aspecifica in WB, dil 1:500
Date published: 2025-05-30
Rated 4 out of 5 by from Good resul with Huh7 cell lysatesTested on Western blot using whole cell lysates (Huh7 cell line) with good results (good signal, no background). Application: Western blot Sample: whole lysate of human hepatocytes from Huh7 cell line Gel running conditions: reduced, denaturing, 4-12% SDS-PAGE Loading amount: 30 µg protein/lane Transfer: semi-dry, on nitrocellulose membrane (0.45 µm) Blocking agent: 5% milk in TBST buffer, for 1h, RT Primary antibody dilution- 1: 500 in 5% milk, for 16 h at 4°C Secondary antibody: non- Santa Cruz antibody anti-mouse-HRP, 1:10000 dilution in 5% milk, for 1h, RT Detection: ECL substrate (femto sensitivity) Exposure time: 1 sec Bands: a strong, band at approx. 60 kDa (probably the HMGCR C-terminal cleavage products); fade, non-specific bands at 35 kDa.
Date published: 2023-08-04
Rated 4 out of 5 by from good!Ho ricevuto il campione omaggio funziona bene. Bande nitide e chiare
Date published: 2021-04-05
Rated 1 out of 5 by from No WB signalI have tested this Ab 1:500 in a solution 1:500 in milk 5% in TBS-Tween, O.N. and I obtained no signal in either of the 6 total protein extract from different ovarian cancer cell lines
Date published: 2021-02-10
Rated 4 out of 5 by from Nice antibody- some non specific bindingNice antibody that I tried on mouse liver lysate. There are many bands that come up but I would use it again.
Date published: 2018-04-26
Rated 4 out of 5 by from wb用途很顺利我2个月前购买了这个产品，使用效果较好，实验顺利。应用用途为WB，使用反馈良好，之后会继续光顾santa抗体。
Date published: 2018-02-01
Rated 1 out of 5 by from Did not work in WBI used this Ab in WB using lysates from CHO cells (chinese hamster ovary). Although the product is recommended, there was no band detected in the range of 90-130 kD. There were only fuzzy, non-specific bands at 230 kD. Rest of the antibodies I used in the same blot worked just fine. I did not use it for IF.
Date published: 2017-12-07
Rated 1 out of 5 by from Didn't work in my handsI wouldn't recommend it. I didn't get any bands, but also didn't bother to troubleshoot ver much so who knows.
Date published: 2017-11-08
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HMGCR 抗体 (C-1) is rated 3.6 out of 5 by 21.
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