Date published: 2025-9-8
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His-Tag Antibody (HIS.H8) - Western Blotting - Image 15066
His-Tag 抗体 (HIS. H8): sc-57598. 小鼠重组 His-Tag 标记 p27 的 Western 印迹分析.
His-Tag Antibody (HIS.H8) - Western Blotting - Image 15066
His-Tag 抗体 (HIS. H8): sc-57598. 小鼠重组 His-Tag 标记 p27 的 Western 印迹分析.
His-Tag 抗体 (HIS. H8): sc-57598. 小鼠重组 His-Tag 标记 p27 的 Western 印迹分析.

His-Tag 抗体 (HIS.H8): sc-57598

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寫評論提問

说明书
  • His-Tag 抗体 HIS.H8 是小鼠单克隆 IgG2b, 在35篇文献中引用，提供 100 µg/ml
  • 针对6x His标签的多肽
  • 推荐用于 fusion proteins encoded by polyhistidine expression vectors WB, IP 和 IF检测
  • 看 His-Tag (H-3): sc-8036，可查看有标记的 His-Tag 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取His-Tag (HIS.H8): sc-57598的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是His-Tag Antibody (HIS.H8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与His-Tag Antibody (HIS.H8) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

His-Tag抗体（HIS.H8）是一种针对6x His标签的多肽的小鼠单克隆IgG2b抗体，适用于蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）等应用。His标签由重组蛋白N端或C端添加的六个组氨酸残基序列组成，其结构对其功能至关重要。组氨酸的咪唑侧链对镍和钴等金属离子具有极强的亲和力，这使得在固定金属亲和色谱（IMAC）过程中，His标签蛋白能够与金属螯合树脂高效结合。这种结构特性使得重组蛋白的纯化能够一步完成，同时保持其天然构象和功能。His标签体积小，对蛋白质结构和活性干扰最小，因此成为蛋白质表达研究的首选。了解His标签的结构和功能对于从事蛋白质纯化、检测和分析的研究人员来说非常重要。His标签单克隆抗体（HIS.H8）可特异性结合His标签序列，有助于在各种实验条件下对His标签蛋白进行灵敏、特异性的检测。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

His-Tag 抗体 (HIS.H8) 参考文献:

  1. His标签重组蛋白色谱纯化中咪唑色谱回收的评估。  |  Noubhani, AM., et al. 2003. J Chromatogr B Analyt Technol Biomed Life Sci. 790: 153-9. PMID: 12767328
  2. 恒河猴中His标签重组人内皮抑素的药代动力学。  |  Song, HF., et al. 2005. Acta Pharmacol Sin. 26: 124-8. PMID: 15659125
  3. 通过His标签将酶高效固定于微通道表面并应用于微反应器。  |  Miyazaki, M., et al. 2005. Protein Pept Lett. 12: 207-10. PMID: 15723648
  4. 改良的His标签载体，用于生产重组蛋白激酶。  |  Lomas-Lopez, R., et al. 2008. Anal Biochem. 377: 272-3. PMID: 18371293
  5. 一种用于纯化His标签生物纳米胶囊的亲和色谱法。  |  Nishimura, Y., et al. 2013. J Virol Methods. 189: 393-6. PMID: 23523889
  6. 磷脂酰肌醇-3-激酶催化亚基上连接的N端His标签对酶活性的影响。  |  Dickson, JM., et al. 2013. Biosci Rep. 33: PMID: 23968392
  7. 在毕赤酵母中优化表达，消除后续His标签纯化中的常见蛋白污染物。  |  Chen, Y., et al. 2014. Biotechnol Lett. 36: 711-8. PMID: 24322766
  8. 在大肠杆菌中通过亚细胞不均匀分布，过表达带有N端His标签的重组蛋白。  |  Park, WJ., et al. 2015. Acta Biochim Biophys Sin (Shanghai). 47: 488-95. PMID: 25994007
  9. 用于重组蛋白检测和基于 T 细胞的免疫疗法的新型 His-tag 结合适配体  |  Yang, LT., et al. 2023. Talanta. 263: 124722. PMID: 37247456
  10. N-末端LysSN-His-标签可提高枯草芽孢杆菌中细胞内重组蛋白的产量。  |  Le, NTP., et al. 2023. Cell Biochem Funct. 41: 823-832. PMID: 37515537

订购信息

产品名称产品编号规格价格数量收藏夹

His-Tag 抗体 (HIS.H8)

sc-57598
100 µg/ml
$316.00

His-Tag (HIS.H8): m-IgG2b BP-HRP 套装

sc-548437
100 µg Ab; 10 µg BP
$354.00

why my protein doesn't show up from WB membrane, i have been used antibody anti his HIS.H8 (st.cruz) 1st antibody and mouse HRP conjugate (st cruz) as a 2nd antibody..what is wrong? is the 1st antibody not compatible with 2nd antibody? thank you

Asked by: rindria
Thank you for your question. This is a mouse monoclonal primary antibody, and we recommend using anti-mouse IgG as a secondary detection antibody. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

What is the best secondary to use with His-probe Antibody (HIS.H8): sc-57598 in Western blot?

Asked by: cjMara
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2018-03-31
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Rated 5 out of 5 by from very GoodVery strong and less background even when incubated at room temp.
Date published: 2018-03-29
Rated 5 out of 5 by from Strong WBNice western data; strong, specjfjc signal with low background !!
Date published: 2017-01-26
Rated 5 out of 5 by from Reliable AntibodyRaised against a 6x HIS tagged protein but works on any HIS tagged polypeptide. Very strong and clear signal via WB.
Date published: 2017-01-09
Rated 4 out of 5 by from Reported to work well in Western Blot usingReported to work well in Western Blot using transfected HNSCC cells. -SCBT Publication Review
Date published: 2015-04-02
Rated 4 out of 5 by from Good data obtained via IF using human transfectedGood data obtained via IF using human transfected HUVEC cells. -SCBT Publication Review
Date published: 2015-03-29
Rated 4 out of 5 by from Success was had using product scSuccess was had using product sc-57598 in Western Blot using transfected 293T cells. -SCBT Publication Review
Date published: 2015-02-25
Rated 3 out of 5 by from StrongStrong, crisp band observed by Western Blot using a His-probe-tagged p27 protein. -SCBT QC
Date published: 2014-02-03
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His-Tag 抗体 (HIS.H8) is rated 4.3 out of 5 by 7.
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