His-Tag抗体 (H-3) 推荐用于 fusion proteins encoded by polyhistidine expression vectors WB 和 IP检测

His-Tag 抗体 (H-3): sc-8036

His-Tag抗体 (H-3) is rated 4.6 out of 5 by 40.
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说明书

    • 抗-His-Tag 抗体 H-3 是小鼠单克隆 IgG1 (kappa轻链),His-Tag抗体,规格为200 µg/ml
    • raised against a His-tagged recombinant protein
    • His-Tag 抗体 (H-3) 推荐用于 WB 和 IP,检测 fusion proteins encoded by polyhistidine expression vectors
    • 抗-His-Tag 抗体 (H-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 另提供生物素偶联抗体,可用于WB、IHC(P)和ELISA实验
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取His-Tag (H-3): sc-8036免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I'd like to know this Ab is working both N-terminal and C-terminal 6xHis ?

Asked by: Hiroki
Thank you for your question. This antibody was raised against a His tagged recombinant protein. Unfortunately the exact epitope has not been mapped, so we do not have further information.
Answered by: Tech Service
Date published: 2019-08-21

What buffer should i use to dilute the antibody? can the antibody be stored in that buffer?

Asked by: yanale
Thank you for your question. This depends on the application you are using the antibody for, so it is difficult to answer. For Western blotting we recommend 5% milk with TBS-T, and for IHC we would recommend using a blocking serum or reagent. You can find this information in our recommended protocols which you can find here: https://www.scbt.com/scbt/resources/protocols
Answered by: Tech Service
Date published: 2019-04-29

Can this antibody pick up 10His-tagged proteins?

Asked by: wei UTHSC
Thank you for your question. We have not tested this antibody for the detection of 10-His-tagged proteins, but it is possible that it would work fine. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-02-25

What is the volume provided of this antibody? I noticed that you don't provide final volume for any of your anti-HIS antibodies. Are all your antibody prices for a specific amount?

Asked by: Labrat
Thank you for your question. We provide a total of 200 µg IgG in 1 ml final volume. Pricing is available on our website.
Answered by: Technical Support
Date published: 2019-02-23

Hi, for the His-probe Antibody (H-3): sc-8036. If I want to use it in a ELISA assay, what is the recommend concentration? Thank you

Asked by: Jackie1029
Thank you very much for your question. We suggest this antibody at a starting dilution of 1:50 to test antibody samples and controls at 50 µl/well diluted in blocking buffer. Antibodies may be serially diluted for determining the titer. Our ELISA protocol: https://www.scbt.com/scbt/resources/protocols/elisa-assays
Answered by: Technical Support Europe
Date published: 2018-01-11

Thank you very much for the below comments on using these Ab for ELISA. So what is the recommended dilution that I can start with ?

Asked by: Oleg
Thank you for your question. We would recommend starting with a high concentration of antibody, or a 1:100 dilution, but we recommend optimizing for the best antibody to antigen concentration through titration. A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/elisa-assays
Answered by: Technical Support
Date published: 2017-11-06

Hello, I am wondering if these Ab can be used for ELISA? -if yes, what is the recommended dilution? if not, what His Ab are recommended -and what secondary Ab conjugated to HRP can you recommend ? Thank you Kind regards Oleg Tyurin

Asked by: Oleg
Thank you for your question. Yes, you can use this mouse monoclonal antibody, His-probe (H-3): sc-8036, for ELISA. This primary antibody is a mouse monoclonal IgG1 so you could use any anti-mouse IgG secondary antibody for detection. We recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102. A link is provided below as a reference. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Technical Support
Date published: 2017-11-02

Does sc-8036 AF488 contain a vial of His Peptide, in addition to the probe? Tx.

Asked by: Goenuel
Thank you for your question. We do not sell a corresponding blocking peptide to be used in conjunction with this mouse monoclonal His-probe Antibody (H-3): sc-8036.
Answered by: Technical Support
Date published: 2017-09-20
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Rated 5 out of 5 by from Works perfectly in Western Blot Used His-probe H-3 HRP for detection of His-tagged protein as 1:500 dilution in 1xTBST/5% skim milk over night at 4°C
Date published: 2019-07-09
Rated 5 out of 5 by from Sehr gute Ergebnisse im WB und ELISA. Sehr gute Ergebnisse im WB und ELISA. Sehr zufrieden.
Date published: 2019-07-05
Rated 5 out of 5 by from Excellent Highly specific, strong bands, dilution in WB used 1:5000.
Date published: 2018-10-23
Rated 5 out of 5 by from Muy buenos resultados Encantados con los resultados y con el servicio prestado por la empresa
Date published: 2018-04-12
Rated 2 out of 5 by from Poor signal I tried a 10 ug sample of His probe antibody to detect 6 His tagged protein. A 1:100 dilution was used including overnight incubation. Alkaline-phosphatase linked second antibody was used. 50 ug of his-tagged protein was added. The signal was very poor.
Date published: 2018-03-26
Rated 5 out of 5 by from Great features I bought this a month ago and am so happy that I did
Date published: 2018-02-05
Rated 5 out of 5 by from Very Clean Antibody! Gave strong signal on WBs for His tagged proteins expressed in insect cells.
Date published: 2018-01-31
Rated 4 out of 5 by from Good results The is a good antibody to recognize his tag products.
Date published: 2017-12-12
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