Hic-5抗体 (C-6) 推荐用于 mouse, rat 和 human 来源的Hic-5 WB, IP, IF, IHC(P) 和 ELISA检测

Hic-5 抗体 (C-6): sc-271353

Hic-5抗体 (C-6) is rated 4.8 out of 5 by 5.
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说明书

    • 抗-Hic-5 抗体 C-6 是小鼠单克隆 IgG2b (kappa轻链),Hic-5抗体,规格为200 µg/ml
    • 特异性抗原位于human物种的Hic-5的C-terminus附近的氨基酸409-434之间
    • 抗-Hic-5 抗体 (C-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Hic-5; 也和以下物种反应,包括: 和 equine, canine and bovine
    • 可用于ChIP的TransCruz试剂(sc-271353 X, 200 µg/0.1 ml)
    • 抗-Hic-5 抗体 (C-6) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Hic-5 (C-6): sc-271353免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG2b BP-HRP 是Hic-5 Antibody (C-6) for WB and IHC(P)首选应用二抗. 这些试剂现在以套装形式提供Hic-5 Antibody (C-6) (见下方订购信息).
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What application is the blocking peptide sc-271353 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from It works! Tried it in western blot and IP using retina lysates. It worked in both types of experiments, each time the detected bands were strong and specific, detected the expected size porteins.
Date published: 2018-05-11
Rated 4 out of 5 by from Work for Western blot Using hASMC extract, detect a band at 50 kD. But also detect a band around 75 kD.
Date published: 2017-11-14
Rated 5 out of 5 by from Specific and robust in WB This is a nice and strong Hic-5 monoclonal antibody that works great in Western blot. Highly recommend this clone!
Date published: 2017-02-07
Rated 5 out of 5 by from Great Western Blot data of Hic Great Western Blot data of Hic-5 expression in non-transfected and mouse Hic-5 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Satisfactory Western Blot data of Hic Satisfactory Western Blot data of Hic-5 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2014-01-02
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