Hemoglobin gamma Antibody (B-4): sc-377395

Hemoglobin γ Antibody (B-4) is a mouse monoclonal IgG3 antibody specific for an epitope mapping between amino acids 9-43 near the N-terminus of human Hemoglobin gamma. Hemoglobin gamma, also known as fetal gamma globin or HBG1/HBG2, is a crucial component of fetal hemoglobin (Hb F), which consists of two alpha (α) chains and two gamma (γ) chains. During fetal development, Hemoglobin gamma interacts with alpha globin chains to form Hb F tetramers, facilitating efficient oxygen transport from the mother to the fetus due to its higher affinity for oxygen compared to adult hemoglobin. Proteins such as BCL11A and KLF1 interact indirectly with Hemoglobin gamma by regulating its gene expression. BCL11A acts as a repressor that silences gamma globin expression after birth, leading to the switch from fetal to adult hemoglobin. KLF1, a transcription factor, also plays a role in this switch by activating BCL11A expression, thereby contributing to the repression of gamma globin genes. Understanding these interactions is important for developing therapeutic strategies for hemoglobinopathies like sickle cell disease and beta-thalassemia, where reactivation of gamma globin expression can ameliorate disease symptoms. Hemoglobin γ (B-4) antibody is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications, making it a valuable tool for studying the role and regulation of Hemoglobin gamma in human biology.

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Hemoglobin gamma Antibody (B-4) References:

1. N-terminal contributions of the gamma-subunit of fetal hemoglobin to its tetramer strength: remote effects at subunit contacts.  |  Yagami, T., et al. 2002. Protein Sci. 11: 27-35. PMID: 11742119
2. Analysis of hemoglobin gamma chain in patients with Cooley's anemia in Taiwan.  |  Lin, LI., et al. 1992. J Formos Med Assoc. 91: 121-5. PMID: 1285319
3. Quantitative analysis of cellular fetal hemoglobin gamma chain messenger RNA (HbF-gamma mRNA) in maternal peripheral blood.  |  Okuda, T., et al. 2004. Prenat Diagn. 24: 881-6. PMID: 15565590
4. Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.  |  Gorczynski, RM., et al. 2005. Immunol Lett. 100: 120-9. PMID: 16154492
5. Induction of tolerogenic vs immunogenic dendritic cells (DCs) in the presence of GM-CSF is regulated by the strength of signaling from monophosphoryl lipid A (MPLA) in association with glutathione and fetal hemoglobin gamma-chain.  |  Khatri, I., et al. 2009. Immunol Lett. 124: 44-9. PMID: 19379773
6. Binding studies of hydroxylated Multi-Walled Carbon Nanotubes to hemoglobin, gamma globulin and transferrin.  |  Sekar, G., et al. 2015. J Photochem Photobiol B. 153: 222-32. PMID: 26432959
7. Heterogeneity of hemoglobin gamma chains in normal newborns and in cases of alpha and beta thalassemia.  |  Lie-Injo, LE., et al. 1979. Am J Hematol. 6: 17-25. PMID: 453194
8. [The primary structure of the hemoglobin gamma-chains of fetal sheep (Ovis ammon) and goat (Capra aegagrus), Artiodactyla].  |  Kleinschmidt, T. and Braunitzer, G. 1982. Hoppe Seylers Z Physiol Chem. 363: 789-96. PMID: 7118074
9. The isolation of the gamma subunit of fetal hemoglocin (HbF) and its use in a radioimmunoassay for HbF.  |  James, RF., et al. 1980. J Immunol Methods. 32: 251-60. PMID: 7351487
10. German population data on the loci low-density-lipoprotein receptor, glycophorin A, hemoglobin gamma G, D7S8 and group-specific component.  |  Huckenbeck, W., et al. 1995. Anthropol Anz. 53: 193-8. PMID: 7486878

The preferred Hemoglobin β antibody is Hemoglobin β Antibody (37-8): sc-21757.