Date published: 2025-9-7
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Hemoglobin β Antibody (37-8) - Western Blotting - Image 375303
血红蛋白 β 抗体 (37-8): sc-21757. TF-1 (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液中血红蛋白 β 表达的 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.
血红蛋白 β 抗体 (37-8): sc-21757. TF-1 (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液中血红蛋白 β 表达的 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.

Hemoglobin β 抗体 (37-8): sc-21757

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说明书
  • Hemoglobin beta 抗体 37-8 是小鼠单克隆 IgG1 κ，Hemoglobin β抗体, 在78篇文献中引用，规格为200 µg/ml
  • 针对human血红蛋白的
  • Hemoglobin beta 抗体 (37-8) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 Hemoglobin β
  • 抗Hemoglobin beta抗体（37-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供PerCP 偶联抗体，可用于IF、IHC(P)和FCM实验
  • 关于如何获取Hemoglobin β (37-8): sc-21757的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Hemoglobin β 抗体 (37-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Hemoglobin β 抗体 (37-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Hemoglobin 抗体 包括 Hemoglobin α, Hemoglobin β, Hemoglobin δ, Hemoglobin ε, Hemoglobin γ和Hemoglobin ζ

血红蛋白β抗体（37-8）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对人类血红蛋白β。血红蛋白β单克隆抗体（37-8）可检测人类样本中的血红蛋白β，也称为血红蛋白亚基β、β-珠蛋白或CD113t-C，检测方法包括：蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）。血红蛋白β是血红蛋白的重要组成部分，血红蛋白是红细胞中携带氧气的蛋白质，负责将氧气从肺部输送到组织，并促进二氧化碳返回肺部。血红蛋白分子由两个α（α）链和两个β（β）链组成，它们成对排列形成四聚体，每个四聚体与一个血红素基团相关联，该基团含有与氧气结合的铁离子。α链和β链之间的相互作用对于血红蛋白的结构完整性和氧结合特性至关重要。血红蛋白β基因的突变可导致血红蛋白病，如镰状细胞性贫血和β地中海贫血，这凸显了在医学研究和诊断中研究这种蛋白质的重要性。血红蛋白β（37-8）抗体有未结合和结合两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，为各种实验应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Hemoglobin β 抗体 (37-8) 参考文献:

  1. 在无细胞系统中表达的人类血红蛋白（Hb）β-和γ-球蛋白链与α-球蛋白链组装成 Hb A 和 Hb F。  |  Adachi, K., et al. 2002. J Biol Chem. 277: 13415-20. PMID: 11827978
  2. 镰状血红蛋白纤维组装中相互作用的联系：α-链 AB 区突变产生的抑制作用被其 EF 角的突变所抵消。  |  Sudha, R., et al. 2004. J Biol Chem. 279: 20018-27. PMID: 14982923
  3. 人类α-血红蛋白通过其伴侣蛋白转移到β-血红蛋白：一种新状态的证据。  |  Baudin-Creuza, V., et al. 2004. J Biol Chem. 279: 36530-3. PMID: 15220346
  4. AHSP 介导稳定α-血红蛋白的分子机制。  |  Feng, L., et al. 2004. Cell. 119: 629-40. PMID: 15550245
  5. β地中海贫血中胎儿血红蛋白的调节：异质性、修饰剂和治疗方法。  |  Sripichai, O. and Fucharoen, S. 2016. Expert Rev Hematol. 9: 1129-1137. PMID: 27801605
  6. 四极杆飞行时间质谱法鉴定β地中海贫血患者血红蛋白未知峰中的α、β、γ和δ亚基。  |  Abdullah, UYH., et al. 2019. Hemoglobin. 43: 182-187. PMID: 31298599
  7. 通过氢交换研究血红蛋白β链C末端变构能。  |  Louie, G., et al. 1988. J Mol Biol. 201: 755-64. PMID: 3172203
  8. 血红蛋白亚基β蛋白作为犯罪现场血迹沉积时间的新标记。  |  Heo, TM., et al. 2022. Forensic Sci Int. 336: 111348. PMID: 35635979
  9. 人类 zeta-globin 基因复合物长度多态性的分子基础。  |  Goodbourn, SE., et al. 1983. Proc Natl Acad Sci U S A. 80: 5022-6. PMID: 6308667
  10. 人类α-球蛋白α1和α2基因座的同源性和协同进化。  |  Liebhaber, SA., et al. 1981. Nature. 290: 26-9. PMID: 7010180
  11. 人类血红蛋白βA和βS链与α链亲和力不同的证据。  |  Shaeffer, JR. 1980. J Biol Chem. 255: 2322-4. PMID: 7358673
  12. 血红蛋白的多重功能  |  Giardina, B., et al. 1995. Crit Rev Biochem Mol Biol. 30: 165-96. PMID: 7555018

订购信息

产品名称产品编号规格价格数量收藏夹

Hemoglobin β 抗体 (37-8)

sc-21757
200 µg/ml
$316.00

Hemoglobin β (37-8): m-IgG Fc BP-HRP 套装

sc-526611
200 µg Ab; 10 µg BP
$354.00

Hemoglobin β (37-8): m-IgG1 BP-HRP 套装

sc-531984
200 µg Ab; 20 µg BP
$354.00

Hemoglobin β 抗体 (37-8) AC

sc-21757 AC
500 µg/ml, 25% agarose
$416.00

Hemoglobin β 抗体 (37-8) HRP

sc-21757 HRP
200 µg/ml
$316.00

Hemoglobin β 抗体 (37-8) FITC

sc-21757 FITC
200 µg/ml
$330.00

Hemoglobin β 抗体 (37-8) PE

sc-21757 PE
200 µg/ml
$343.00

Hemoglobin β 抗体 (37-8) Alexa Fluor® 488

sc-21757 AF488
200 µg/ml
$357.00

Hemoglobin β 抗体 (37-8) Alexa Fluor® 546

sc-21757 AF546
200 µg/ml
$357.00

Hemoglobin β 抗体 (37-8) Alexa Fluor® 594

sc-21757 AF594
200 µg/ml
$357.00

Hemoglobin β 抗体 (37-8) Alexa Fluor® 647

sc-21757 AF647
200 µg/ml
$357.00

Hemoglobin β 抗体 (37-8) Alexa Fluor® 680

sc-21757 AF680
200 µg/ml
$357.00

Hemoglobin β 抗体 (37-8) Alexa Fluor® 790

sc-21757 AF790
200 µg/ml
$357.00

Hemoglobin β 抗体 (37-8) PerCP

sc-21757 PerCP
100 tests in 2 ml
$351.00

I am looking for an antibody that could recognize the haemoglobin beta with sickle cells mutation. could you tell me if the residue Glu6 is included in the antigen for the following antibody? Hemoglobin β Antibody (37-8) and (21): sc-130321

Asked by: laura
Thank you for your question. Hemoglobin β Antibody (21): sc-130321 and Hemoglobin β Antibody (21): sc-130321 were raised against recombinant Hemoglobin β of human origin. No epitope mapping has been performed for either antibody, so their exact binding sites are not known at this point, unfortunately.
Answered by: Tech Support Europe
Date published: 2024-12-16

Hi what dilution do you suggest for flow cytometry?

Asked by: bart
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-28

What is the HBB epitope that Hemoglobin β Antibody (37-8) targets to HBB?

Asked by: ASCtherapeutics
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-09-20

Which part of sequence of human beta hemoglobin was used to generate Hemoglobin β Antibody (37-8)? Can this antibody detect N-terminal of human beta hemoglobin

Asked by: Ming87
Thank you for your question. The exact antigenic sequence used to develop this mouse monoclonal antibody, Hemoglobin β (37-8): sc-21757, is not known. The epitope recognized has also not been determined so we can not advise you whether or not the antibody is able to detect a specific N-terminal sequence of human beta hemoglobin.
Answered by: Technical Support
Date published: 2017-12-07

What dilution should be used with Hemoglobin β Antibody (37-8) for IHC applications?

Asked by: Trav11
The recommended starting dilution for Hemoglobin β Antibody (37-8): sc-21757 for immunohistochemistry is 1:50, with a range of 1:50-1:500. This information, along with more technical data, can be found under the datasheet link at the top of the page: https://datasheets.scbt.com/sc-21757.pdf If you have any further questions, please feel free to contact the Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-09-15
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Rated 5 out of 5 by from good antibodyEmployed with 20 ug of human erythroid progenitor cells (1:1000 dilution), it gives clean and specific band.
Date published: 2023-01-11
Rated 5 out of 5 by from very specificvery good. No non-specific and very strong signal. Had to dilute sample many folds. Detect both the monomer and the dimeric forms in both HSC-derived cells and RBC
Date published: 2022-12-12
Rated 5 out of 5 by from Good antibodyUsed 1:1000 it worked very well, we have not seen any not specific band and the result was very clean (using 20 ug of protein extracted from cultured erythroblasts)
Date published: 2021-06-28
Rated 5 out of 5 by from Great for Western blotAntibody works really well for Western blot at 1:2000 dilution. Strong, specific band ~15 kDa. I got this antibody as a sample and will now be buying it.
Date published: 2017-08-09
Rated 5 out of 5 by from Excellent!Works good at 1:200 dilution to detect β Hemoglobin in human erythroid cell line.
Date published: 2017-07-19
Rated 5 out of 5 by from good qualitygood quality controled and high specificity for wb,at1:500.
Date published: 2017-06-11
Rated 5 out of 5 by from very nice antibody for WB and IFI have used this antibody for WB and IF, it is a very nice antibody against HBB protein. I will always use it all the time in the future!
Date published: 2017-04-29
Rated 5 out of 5 by from Great beta globin antibodyGreat antibody that produces strong results in flow cytometry, IHC, and Western blot in human samples.
Date published: 2017-01-06
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Hemoglobin β 抗体 (37-8) is rated 5.0 out of 5 by 16.
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