Date published: 2025-9-7
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Histone Deacetylase 6 (HDAC6) Antibody (D-11) - Western Blotting - Image 9884
HDAC6 抗体 (D-11): sc-28386. K-562 全细胞裂解液 (A) 和 K-562 (B), Jurkat (C) 和 HeLa (D) 核提取物中 HDAC6 表达的 Western 印迹分析.
HDAC6 抗体 (D-11): sc-28386. K-562 全细胞裂解液 (A) 和 K-562 (B), Jurkat (C) 和 HeLa (D) 核提取物中 HDAC6 表达的 Western 印迹分析.

Histone Deacetylase 6 (HDAC6) 抗体 (D-11): sc-28386

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寫評論提問

说明书
  • Histone Deacetylase 6 (HDAC6) 抗体 D-11 是小鼠单克隆 IgG2a κ，Histone Deacetylase 6 (HDAC6)抗体, 在51篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HDAC6的氨基酸916-1215
  • Histone Deacetylase 6 (HDAC6) 抗体 (D-11) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 HDAC6
  • 抗Histone Deacetylase 6 (HDAC6)抗体（D-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Histone Deacetylase 6 (HDAC6) (D-11): sc-28386的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Histone Deacetylase 6 (HDAC6) 抗体 (D-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Histone Deacetylase 6 (HDAC6) 抗体 (D-11) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HDAC 抗体 包括 HDAC1, HDAC10, HDAC11, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7和HDAC8

HDAC6抗体（D-11）是一种鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人类HDAC6蛋白。HDAC6 (D-11) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。HDAC6 通过脱乙酰化非组蛋白蛋白来调节细胞过程，从而发挥关键作用，这会影响各种信号通路和细胞功能。HDAC6 (D-11) 单克隆抗体可识别一种参与细胞骨架调节的蛋白质，该蛋白质对于微管的正常功能至关重要，而微管对于细胞形状、运动和细胞内运输至关重要。HDAC6能够与微管蛋白相互作用并使其脱乙酰化，这凸显了其在维持细胞结构和功能方面的重要性。HDAC6失调与多种疾病有关，包括癌症和神经退行性疾病，这使得HDAC6成为治疗干预的重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) 参考文献:

  1. 三种蛋白定义了一类与酵母 Hda1p 有关的人类组蛋白去乙酰化酶。  |  Grozinger, CM., et al. 1999. Proc Natl Acad Sci U S A. 96: 4868-73. PMID: 10220385
  2. HDAC6在自噬和NLRP3炎症小体中的作用。  |  Chang, P., et al. 2021. Front Immunol. 12: 763831. PMID: 34777380
  3. HDAC6的中央抑制作用可重新激活肥胖症中的瘦素信号，从而促进深度减肥。  |  Guan, D., et al. 2024. Cell Metab. 36: 857-876.e10. PMID: 38569472
  4. 人类HDAC6通过感知缬氨酸的丰度来调节DNA损伤。  |  Jin, J., et al. 2024. Nature.. PMID: 39567688
  5. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  6. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  7. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  8. 一种与酵母转录调节因子 Rpd3p 相关的哺乳动物组蛋白去乙酰化酶。  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
  9. 核糖体 DNA 调节人类 Hat1 乙酰转移酶的核心-活素结合亚基。  |  Verreault, A., et al. 1998. Curr Biol. 8: 96-108. PMID: 9427644
  10. 人类 RPD3 同源物 HDAC3 的特征。  |  Emiliani, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2795-800. PMID: 9501169
  11. 转录激活剂将组蛋白乙酰转移酶复合物导向核糖体。  |  Utley, RT., et al. 1998. Nature. 394: 498-502. PMID: 9697775
  12. 组蛋白乙酰转移酶和去乙酰化酶在基因调控中的作用。  |  Kuo, MH. and Allis, CD. 1998. Bioessays. 20: 615-26. PMID: 9780836

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 6 抗体 (D-11)

sc-28386
200 µg/ml
$316.00

Histone Deacetylase 6 (HDAC6) (D-11): m-IgG Fc BP-HRP 套装

sc-525530
200 µg Ab; 10 µg BP
$354.00

Histone Deacetylase 6 (HDAC6) (D-11): m-IgG2a BP-HRP 套装

sc-546306
200 µg Ab; 10 µg BP
$354.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) AC

sc-28386 AC
500 µg/ml, 25% agarose
$416.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) HRP

sc-28386 HRP
200 µg/ml
$316.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) FITC

sc-28386 FITC
200 µg/ml
$330.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) PE

sc-28386 PE
200 µg/ml
$343.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) Alexa Fluor® 488

sc-28386 AF488
200 µg/ml
$357.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) Alexa Fluor® 546

sc-28386 AF546
200 µg/ml
$357.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) Alexa Fluor® 594

sc-28386 AF594
200 µg/ml
$357.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) Alexa Fluor® 647

sc-28386 AF647
200 µg/ml
$357.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) Alexa Fluor® 680

sc-28386 AF680
200 µg/ml
$357.00

Histone Deacetylase 6 (HDAC6) 抗体 (D-11) Alexa Fluor® 790

sc-28386 AF790
200 µg/ml
$357.00

请问可以和大鼠心肌细胞H9C2反应吗

Asked by: catlli
感谢您的提问。 Histone Deacetylase 6 (HDAC6) Antibody (D-11)，推荐的种属反应只有人，无大鼠。 Thank you for your question. Histone Deacetylase 6 (HDAC6) Antibody (D-11) is recommended for detection of HDAC6 of human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Ling Chen
Date published: 2024-09-18

Hello, I just want to realice a single test and I only need a few microliters of this antibody, do you have little and less expensive vials?

Asked by: Evangelina
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

Does this antibody work for mouse tissue (cryosections) IF ?

Asked by: Kate34
Thank you for your question. We do not recommend using this HDAC6 Antibody (D-11): sc-28386 for detection of HDAC6 of mouse origin. It is currently only recommended for detection of HDAC6 of human origin. It is suitable for IF on cryosections for human tissue. Unfortunately, at this time, we do not offer any alternative antibodies against HDAC6 that are recommended for use with mouse samples. Please check back with us in the future as we are constantly adding new antibodies to our product line.
Answered by: Technical Support
Date published: 2023-02-22

What is the total volume of HDAC6 Antibody (D-11): sc-28386 provided?

Asked by: Anna99
Each vial contains 200 µg of mouse monoclonal IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 2 out of 5 by from Not so bad!Only after o.n incubation at 4° (1:100 dilution) I obtained a weak signal!
Date published: 2022-04-22
Rated 1 out of 5 by from Did not work in WB!I tried this with GBM cell line Whole cell extracts and could not see any bands. I do the same experiment using another brand's antibody and did obtain results.
Date published: 2018-11-22
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from good quality for WBThis antibody works well and generates a clear signal at the predicted position on the western blot (1:500 dilution, 50ug of rat heart extract).
Date published: 2017-09-21
Rated 4 out of 5 by from Antibody was great for WB and IP.Antibody was great for WB and IP in colon cancer, globlastoma and multiple myeloma cells. I recommond this antibody.
Date published: 2017-06-19
Rated 3 out of 5 by from WB signals not so strongI use 1:200 diluted antibody to blot 60 mg total cell lysates from HCT116, K562 and HeLa but the signals were not as strong as shown in the reviews or the datasheets. Could you help me?
Date published: 2017-01-11
Rated 5 out of 5 by from OutstandingVery nice western blot and IHC data;strong signal !!
Date published: 2017-01-06
Rated 5 out of 5 by from good featuresThis product have a good special features for human source cell line (such as Hela, K562, HCT116) by western blot analysis. I sincerely recomand this prouduct.
Date published: 2017-01-04
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Histone Deacetylase 6 (HDAC6) 抗体 (D-11) is rated 4.3 out of 5 by 15.
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