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HA 标记抗体 (12CA5): sc-57592. HA 标记人重组 JNK 的 Western 印迹分析.
HA-Tag 抗体 (12CA5): sc-57592
4.4(10)
寫評論提問- HA-Tag抗体(12CA5)是小鼠单克隆IgG2b κ, 在242篇文献中引用，规格为100 µg/ml
- 针对重组流感病毒血凝素
- 推荐用于 proteins containing the HA tag WB 和 IP检测
- 看 HA-Tag (F-7): sc-7392，可查看有标记的 HA-Tag 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
- 关于如何获取HA-Tag (12CA5): sc-57592的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成HA-Tag 抗体 (12CA5)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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HA 抗体
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HA-Tag抗体（12CA5）是一种IgG2b κ小鼠单克隆HA-Tag抗体，可通过WB和IP检测含有HA标签的蛋白质。 HA-Tag抗体（12CA5）可作为非偶联抗HA-Tag抗体形式提供。用于在细菌、昆虫和哺乳动物宿主中表达真核基因编码区的质粒载体是常用的；这种表达载体经常用于编码由真核靶蛋白和专门设计的区域组成的杂合融合蛋白，以帮助靶蛋白的纯化和可视化。例如，pCDM8表达载体及其衍生物编码靶蛋白和来自流感蛋白血凝素（HA）的11个氨基酸肽之间的融合。 HA表位标签在用特异性针对HA表位标签的抗体检查时，可用于表达的融合蛋白的Western印迹和免疫组织化学定位。
仅限研究使用。不适用于诊断和治疗用途。
针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz®偶联抗体，从而可以省去使用二抗。
圣克鲁斯生物技术的抗体有超过36万的研究引文。抗HA-Tag抗体(12CA5)在各种科学出版物中有242篇引文。
针对哺乳动物靶标蛋白的一抗，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与绵羊、猪、山羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。
HA-Tag 抗体 (12CA5) 规格
替代产品名称: HA-Tag
产品克隆名称: 12CA5
分类: 一抗
Clonality: 单克隆抗体
抗体同型: IgG2b kappa light chain
氨基酸:
抗原表位靶点: HA-probe
抗原表位种属: influenza virus
产品反应性/检测: proteins containing the HA tag
种属反应性/检测: NSR
其它哺乳动物反应性: N/A
其它非哺乳动物反应性: N/A
应用: WB, IP
瓶的信息: Each vial contains 100 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin
研究引文: 242篇各种科学出版物的引文
HA-Tag 抗体 (12CA5)可选的偶联物/形式: N/A
针对非哺乳动物靶点的一抗，如抗HA-Tag抗体 (12CA5)，在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼，非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.
圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗HA-Tag抗体推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation).
我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。
组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。
单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和 TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。
偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。
正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。
優選的 HA-Tag 抗體是 HA-Tag 抗體 (F-7): sc-7392.
订购信息
| 产品名称 | 产品编号 | 规格 | 价格 | 数量 | 收藏夹 | |
HA-Tag 抗体 (12CA5) | sc-57592 | 100 µg/ml | $316.00 |
Is the sc-57592: HA-antibody available conjugated to magnetic beads?
Asked by: Charujack
Thank you for your question. Please contact our Special Orders Department to inquire about availability and to receive a quote for custom orders. Please call our Technical Service Department, or email specialorders@scbt.com.
Answered by: Technical Service 3
Date published: 2020-11-27
Bonjour, Est-il possible d'obtenir un aliquot de l'anticorps: https://www.scbt.com/p/ha-probe-antibody-12ca5? pour tester. Merci!
Asked by: Anonymous
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-02-13
Is sc-57592: HA-probe (12CA5) available conjugated to agarose for IP?
Asked by: cjMara
Thank you for your question. Yes. Please contact one of our Special Orders Coordinators to inquire about the availability of this monoclonal conjugate. Please email specialorders@scbt.com. Or call our Technical Service Department directly at (800) 457-3801 option 2.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 5 out of
5 by
Raptor Lee from
Works great in IHC with cultured myoblastsI bought this antibody two months ago. It works great to detect myoblasts transduced with plasmids expressing HA tag when I do IHC.
Date published: 2023-05-22
Rated 4 out of
5 by
CeciliaM from
Funziona ma con bande non specificheHo provato un campione di questo prodotto per la detezione di una proteina espressa in protoplasti. La detezione della proteina è stata fatta con un metodo colorimetrico (NBT/BCIP). Però, inoltre alla banda desiderata sono stati visibili anche delle bande non specifiche. Soprattutto con una diluzione dell’anticorpo 1:1000 sono visibili queste bande non specifiche. Con la diluzione più alta (1:1500) si è migliorato un po’ il problema.
Date published: 2018-11-15
Rated 5 out of
5 by
BailuXie from
Excellent performanceI asked for it as a free sample, had clean background and a sharp band indicating the HA-tag protein in western blotting
Date published: 2018-10-17
Rated 4 out of
5 by
cousins from
very good antibody for CO-IP experimentThis Ab pulled down about 50% of HA-tagged protein from nuclear lysate of HEK293T after transient overexpression. A few nonspecific bands were observed (lane 2). Nevertheless, it was very good antibody for CO-IP experiment.
Date published: 2017-01-24
Rated 5 out of
5 by
AbPolly from
Excellent detection!Great antibody for detection of HA-tagged proteins!
Date published: 2017-01-10
Rated 4 out of
5 by
Summer0 from
Awesome in WB!!!This antibody works very well in western blot detection.
Date published: 2016-12-18
Rated 4 out of
5 by
Jonathan from
Works very nicely for IF on human samplesWorks very nicely for IF on human samples. -SCBT QC
Date published: 2014-12-24
Rated 4 out of
5 by
Simon from
Published in IP with mouse samplesPublished in IP with mouse samples. -SCBT Publication Review
Date published: 2014-11-04
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HA-Tag 抗体 (12CA5) is rated
4.4 out of
5 by
10.
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版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。