HA-Tag抗体 (F-7) 推荐用于 proteins containing the HA tag WB, IP, IF, FCM 和 ELISA检测

HA-Tag 抗体 (F-7): sc-7392

HA-Tag抗体 (F-7) is rated 4.6 out of 5 by 116.
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说明书

    • 抗-HA-Tag 抗体 F-7 是小鼠单克隆 IgG2a (kappa轻链),HA-Tag抗体,规格为200 µg/ml
    • specific to epitope mapping within an internal region of the the influenza hemagglutinin (HA) protein protein
    • HA-Tag 抗体 (F-7) 推荐用于 WB, IP, IF, FCM 和 ELISA,检测 proteins containing the HA tag
    • 抗-HA-Tag 抗体 (F-7) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联生物素 用于WB, IHC(P) 和ELISA; 偶联TRITC, PerCP, PerCP-Cy5.5 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
    • 可用于ChIP的TransCruz试剂(sc-7392 X, 200 µg/0.1 ml)
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取HA-Tag (F-7): sc-7392免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

sample을 받아서 사용해볼 수 있을까요? -연세대학교 생명공학과

Asked by: Yeseul Choi
Thank you for your question,You can contact your local distributor to request SCBT antibody sample. Customers in Korea may purchase our products through one of our authorized local distributors. Your local distributor(s):Essence Medical Inc. and Koram Biotech Corporation.Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot. Please call us directly at  (86 21) 6093-6350, email [email protected], or contact us by live chat.
Answered by: Technical Service 11
Date published: 2020-04-29

Hi there, I've purchased the mouse monoclonal anti-HA antibody but I can't find info on what the antibody is resuspended in when I buy it. What is the solvent that the antibody is in?

Asked by: The Scientist
Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Tech Service
Date published: 2020-01-21

Hi We are using this anti-HA tag antibody for using Chip assay (F-7X). But recently we have schedule to Co-IP assay for HA tag protein capture at protein G agarose bead (sc-2003). So, can we use this F-7X antibody for Co-IP assay?

Asked by: Swan
Thank you for your question. Yes, this HA-probe antibody, sc-7392, is fine for Co-IP applications.
Answered by: Tech Service
Date published: 2020-01-06

I would like to know if this ab can detect Y Y D V L D Y A or Y Y D V P D Y A via western blot.

Asked by: andriamil
Thank you for your question. The exact epitope of this antibody has not been mapped yet so it is not yet possible to say which motif is recognized. Feel free to contact our technical support directly to arrange for a sample of this antibody to find out whether it is suitable for your application.
Answered by: Technical Support Europe
Date published: 2019-04-23

Does the HA antibody work better in milk or BSA?

Asked by: corgibot
Thank you for your question. We recommend using 5% nonfat milk in TBST (0.05% Tween-20) for blocking, and for incubating both primary and secondary antibodies. You can also purchased this prepared blocking buffer on our website. https://www.scbt.com/scbt/product/tbs-blotto-a
Answered by: Technical Support
Date published: 2019-03-14

What is the storage buffer for the F-7 AC conjugated to agarose beads?

Asked by: steveb37
Thank you for your question. The agarose conjugated antibody, sc-7392 AC, is in PBS with < 0.1% sodium azide.
Answered by: Technical Support
Date published: 2019-02-02

Which are the residues ( aa) of the HA recognize by the HA-probe (F-7): sc-7392 antibody?

Asked by: Endika
Thank you for your question. The amino acid sequence of HA tag which the antibody recognizes is YPYDVPDYA.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-07

Do you have any protocol for HA-PROBE ANTIBODY (F-7) ?

Asked by: elliyi
Thank you for your question. Since I am not sure which application you are using this antibody, sc-7392, for, here is a link to all our protocols: https://www.scbt.com/scbt/resources/protocols Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-11-18
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Rated 4 out of 5 by from works for wb It works well for wb in HK2 cells. I'll try it also for IF
Date published: 2020-10-07
Rated 3 out of 5 by from Great antibody for western Have got free sample of this antibody. Great antibody for western on cells.
Date published: 2019-10-09
Rated 2 out of 5 by from Great IPs but with a higher background We have been using this HA-antibody AC for a long time and it works great! However, higher background (non-specific IPed bands) has recently become a concern.
Date published: 2019-04-15
Rated 5 out of 5 by from Sehr gute Resultate im Western blot In Western blot we used a 1:1000 dilution of the antibody. We got a very good signal.
Date published: 2019-03-01
Rated 5 out of 5 by from Works for IPs This antibody consistently works well for me in IPs.
Date published: 2018-12-10
Rated 5 out of 5 by from Excellent results I asked for it as a free sample, had clean background and a sharp band indicating the HA-tag protein, and will continue to order it.
Date published: 2018-10-17
Rated 5 out of 5 by from Great. I bought this antibody two year ago and we have been using this one for over two years now.
Date published: 2018-10-12
Rated 5 out of 5 by from Great for Co-IP I bought this product in summer and used it for Co-IP. It works great.
Date published: 2018-10-10
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