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HA-Tag Antibody (F-7) - Immunofluorescence - Image 5593 — HA-Tag 抗体 (F-7): sc-7392. 免疫荧光染色甲醇固定的转染有 HA 融合蛋白的 COS 细胞, 显示细胞质染色.

HA-Tag 抗体 (F-7): sc-7392. 免疫荧光染色甲醇固定的转染有 HA 融合蛋白的 COS 细胞, 显示细胞质染色.

HA-Tag 抗体 (F-7): sc-7392

Name: HA-Tag抗体 (F-7)
Brand: SCBT
SKU: sc-7392
Price: 316.0 USD
Availability: InStock

4.6(129)

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说明书

HA-Tag 抗体 F-7 是小鼠单克隆 IgG_2a κ，HA-Tag抗体, 在3,050篇文献中引用，规格为200 µg/ml
特异性针对the influenza hemagglutinin (HA) protein蛋白内部区域的表位图谱
HA-Tag 抗体 (F-7) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测 proteins containing the HA tag
抗HA-Tag抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
还可偶联生物素 用于WB, IHC(P) 以及 ELISA;偶联TRITC 以及 Alexa Fluor^® 405 用于IF, IHC(P) 和 FCM
可用于ChIP的TransCruz试剂(sc-7392 X, 200 µg/0.1 ml)
关于如何获取HA-Tag (F-7): sc-7392的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG_2a BP-HRP和m-IgGκ BP-HRP是HA-Tag Antibody (F-7) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与HA-Tag Antibody (F-7) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(3030)

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HA-Tag 抗体 (F-7) 参考文献:

HAM：一种新的用于体内蛋白质标记的表位标签。 | Herbst, A. and Tansey, WP. 2000. Mol Biol Rep. 27: 203-8. PMID: 11455955
N-连接糖基化作用对肌酸转运蛋白的影响。 | Straumann, N., et al. 2006. Biochem J. 393: 459-69. PMID: 16167890
用于构建代表性质粒 cDNA 文库的优化 cDNA 大小选择和克隆程序。 | Kieffer, BL. 1991. Gene. 109: 115-9. PMID: 1756970
眼白化病1型基因产物OA1在细胞膜中跨越7次。 | Sone, M. and Orlow, SJ. 2007. Exp Eye Res. 85: 806-16. PMID: 17920058
表位标记转录因子IID的纯化。 | Carey, MF., et al. 2010. Cold Spring Harb Protoc. 2010: pdb.prot5450. PMID: 20647354
使用Biacore测量蛋白质-蛋白质相互作用。 | Leonard, P., et al. 2011. Methods Mol Biol. 681: 403-18. PMID: 20978978
通过原子力显微镜在活细胞表面绘制HA标记的蛋白质。 | Formosa, C., et al. 2015. J Mol Recognit. 28: 1-9. PMID: 26046654
使用Biacore测量蛋白质-蛋白质相互作用。 | Leonard, P., et al. 2017. Methods Mol Biol. 1485: 339-354. PMID: 27730561
单步纯化在大肠杆菌中表达的与谷胱甘肽 S-转移酶融合的多肽。 | Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
多功能原核蛋白质表达系统：过量生产, 亲和纯化和选择性检测。 | Kroll, DJ., et al. 1993. DNA Cell Biol. 12: 441-53. PMID: 7686011
通过表位标签对 Rab8 和 Rab10 这两种低分子量 GTP 结合蛋白进行表达和定域。 | Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
用于重组蛋白鉴定和纯化的短多肽标记序列。 | Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P., Urdal, D.L. and Conlon, P.J. 1988. Nature Biotechnology. 6: 1204-1210.

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
HA-Tag 抗体 (F-7)	sc-7392	200 µg/ml	$316.00
HA-Tag (F-7): m-IgG Fc BP-HRP 套装	sc-528181	200 µg Ab; 10 µg BP	$354.00
HA-Tag (F-7): m-IgGκ BP-HRP 套装	sc-520501	200 µg Ab, 40 µg BP	$354.00
HA-Tag (F-7): m-IgG_2a BP-HRP 套装	sc-547038	200 µg Ab; 10 µg BP	$354.00
HA-probe 抗体 (F-7) X	sc-7392 X	200 µg/0.1 ml	$316.00
HA-probe 抗体 (F-7) AC	sc-7392 AC	500 µg/ml, 25% agarose	$416.00
HA-probe 抗体 (F-7) HRP	sc-7392 HRP	200 µg/ml	$316.00
HA-probe 抗体 (F-7) FITC	sc-7392 FITC	200 µg/ml	$330.00
HA-probe 抗体 (F-7) PE	sc-7392 PE	200 µg/ml	$343.00
HA-probe 抗体 (F-7) Alexa Fluor^® 488	sc-7392 AF488	200 µg/ml	$357.00
HA-probe 抗体 (F-7) Alexa Fluor^® 546	sc-7392 AF546	200 µg/ml	$357.00
HA-probe 抗体 (F-7) Alexa Fluor^® 594	sc-7392 AF594	200 µg/ml	$357.00
HA-probe 抗体 (F-7) Alexa Fluor^® 647	sc-7392 AF647	200 µg/ml	$357.00
HA-probe 抗体 (F-7) Alexa Fluor^® 680	sc-7392 AF680	200 µg/ml	$357.00
HA-probe 抗体 (F-7) Alexa Fluor^® 790	sc-7392 AF790	200 µg/ml	$357.00
HA-probe 抗体 (F-7) B	sc-7392 B	200 µg/ml	$320.00
HA-probe 抗体 (F-7) TRITC	sc-7392 TRITC	200 µg/ml	$320.00
HA-probe 抗体 (F-7) Alexa Fluor^® 405	sc-7392 AF405	200 µg/ml	$357.00
HA-probe (F-7) 中和勝肽	sc-7392 P	100 µg/0.5 ml	$68.00

Does the anti HA antibody (F-7) work when diluted in 5% milk? And if the milk is used to for blocking?

Asked by: Jeanie

Thank you for your question. 5%milk can be used to dilute and block. Here is our western blot protocol. https://www.scbt.com/resources/protocols/western-immuno-blotting

Answered by: BlakeJ

Date published: 2025-06-05

Does the biotinylated version of this product contain one biotin per antibody molecule, or multiple biotins per antibody?

Asked by: polishedSlate

Thank you for your question. The molar ratio of biotin to antibody ranges between 3-7 biotin per IgG.

Answered by: BlakeJ

Date published: 2024-11-05

I want the HA-Tag Antibody (F-7): sc-7392 linked with HRP. Please let me know the price and how i can order it?

Asked by: Afzal

Thank you for your question. You can find sc-7392 HRP and pricing on this product page. Please contact our European sales office directly for a quote.

Answered by: Tech Support Europe

Date published: 2024-10-15

Hi, I was wondering if you have recommendations for isotype control used in immunoprecipitation of this product (sc-7392). Looking forward to hearing from you. Thanks,Yaoning

Asked by: Yaoning

Thank you for contacting Santa Cruz Biotechnology . Please see link below for recommended control for IP. https://www.scbt.com/p/normal-mouse-igg2a

Answered by: BlakeJ

Date published: 2023-05-03

I want to detect influenza HA subtupy H7. this antibody ( # sc-7392) to detect influenza HA subtupy H7?

Asked by: Michin_iPS

Thank you for your question. HA-Tag Antibody (F-7) is recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA.

Answered by: Ling Chen

Date published: 2023-04-07

I have an expression plasmid where the HA-taq is located like this: N´- 2xNLS - linker - HA - protein sequence -C´. Can this antibody detect the HA-tag when it is not N- or C-terminal, but in the middle of the protein sequence?

Asked by: Fred99

Thank you for your question. This antibody will also be able to detect internal HA-tag. Depending on the properties of your plasmid, linearization may be required for efficient detection. Please contact our technical service team directly for additional information.

Answered by: Tech Support Europe

Date published: 2022-09-19

Hello, For the WB detection, how many hours of treatment with 1:1000 dilution do you recommend?

Asked by: Lola322

Thank you for your question. We recommend incubating over night at +4 °C. Depending on your samples and experimental setup different dilutions may produce better results on your western blots.

Answered by: Tech Support Europe

Date published: 2020-12-16

sample을 받아서 사용해볼 수 있을까요? -연세대학교 생명공학과

Asked by: Yeseul Choi

Thank you for your question,You can contact your local distributor to request SCBT antibody sample. Customers in Korea may purchase our products through one of our authorized local distributors. Your local distributor(s):Essence Medical Inc. and Koram Biotech Corporation.Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot. Please call us directly at  (86 21) 6093-6350, email asia@scbio.cn, or contact us by live chat.

Answered by: Technical Service 11

Date published: 2020-04-29

HA-Tag 抗体 (F-7) Questions - page 2

Rated 5 out of 5 by 1016sh from anti-HAAnti-HA mouse monoclonal antibody was working very in our Western blotting.

Date published: 2023-10-13

Rated 5 out of 5 by jacky18 from 效果非常好我购买了HA-Tag抗体，做流式效果非常好，此外western，免疫组化也不错，非常推荐各位购买使用

Date published: 2023-08-21

Rated 4 out of 5 by guardalab from It works very wellI tested the small size and it works well on cellular lysates by western blot.

Date published: 2023-06-27

Rated 4 out of 5 by Chu TH from Reliable antibody!I am so happy to use this antibody to perform WB and IHC

Date published: 2023-03-14

Rated 5 out of 5 by fullerrd from IP and co-IPGood detection of tagged proteins expressed in 293 cells. Capable of standard IP and WB in co-IP experiments.

Date published: 2023-01-24

Rated 4 out of 5 by CC lab from Good antibodyIt works well in IF, but not in WB (at least with our construct)

Date published: 2022-08-18

Rated 5 out of 5 by Robertmc from Ease of useVery easy to use. It worked as advertised. Quick delivery.

Date published: 2022-07-12

Rated 5 out of 5 by KlauMi from perfect HA antibodythis antibody works well in immunofluorescence, Western blotting and ChIP

Date published: 2022-03-04

HA-Tag 抗体 (F-7) Reviews - page 2

HA-Tag 抗体 (F-7) is rated 4.6 out of 5 by 129.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

HA-Tag 抗体 (F-7): sc-7392

HA-Tag 抗体 (F-7) 参考文献:

HA-Tag 抗体 (F-7) 规格

替代产品名称: HA-Tag

产品克隆名称: F-7

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG2a kappa light chain

氨基酸: 98-108

抗原表位靶点: the influenza hemagglutinin (HA) protein

抗原表位种属: influenza virus

产品反应性/检测: proteins containing the HA tag

种属反应性/检测: N/A

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, FCM, ELISA

瓶的信息: Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 3,050篇各种科学出版物的引文

HA-Tag 抗体 (F-7)

HA-Tag (F-7): m-IgG Fc BP-HRP 套装

HA-Tag (F-7): m-IgGκ BP-HRP 套装

HA-Tag (F-7): m-IgG2a BP-HRP 套装

HA-probe 抗体 (F-7) X

HA-probe 抗体 (F-7) AC

HA-probe 抗体 (F-7) HRP

HA-probe 抗体 (F-7) FITC

HA-probe 抗体 (F-7) PE

HA-probe 抗体 (F-7) Alexa Fluor® 488

HA-probe 抗体 (F-7) Alexa Fluor® 546

HA-probe 抗体 (F-7) Alexa Fluor® 594

HA-probe 抗体 (F-7) Alexa Fluor® 647

HA-probe 抗体 (F-7) Alexa Fluor® 680

HA-probe 抗体 (F-7) Alexa Fluor® 790

HA-probe 抗体 (F-7) B

HA-probe 抗体 (F-7) TRITC

HA-probe 抗体 (F-7) Alexa Fluor® 405

HA-probe (F-7) 中和勝肽

Does the anti HA antibody (F-7) work when diluted in 5% milk? And if the milk is used to for blocking?

Does the biotinylated version of this product contain one biotin per antibody molecule, or multiple biotins per antibody?

I want the HA-Tag Antibody (F-7): sc-7392 linked with HRP. Please let me know the price and how i can order it?

Hi, I was wondering if you have recommendations for isotype control used in immunoprecipitation of this product (sc-7392). Looking forward to hearing from you. Thanks,Yaoning

I want to detect influenza HA subtupy H7. this antibody ( # sc-7392) to detect influenza HA subtupy H7?

I have an expression plasmid where the HA-taq is located like this: N&acute;- 2xNLS - linker - HA - protein sequence -C&acute;. Can this antibody detect the HA-tag when it is not N- or C-terminal, but in the middle of the protein sequence?

Hello, For the WB detection, how many hours of treatment with 1:1000 dilution do you recommend?

sample을 받아서 사용해볼 수 있을까요? -연세대학교 생명공학과

抗体同型: IgG_2a kappa light chain

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

HA-Tag (F-7): m-IgG_2a BP-HRP 套装

HA-probe 抗体 (F-7) Alexa Fluor^® 488

HA-probe 抗体 (F-7) Alexa Fluor^® 546

HA-probe 抗体 (F-7) Alexa Fluor^® 594

HA-probe 抗体 (F-7) Alexa Fluor^® 647

HA-probe 抗体 (F-7) Alexa Fluor^® 680

HA-probe 抗体 (F-7) Alexa Fluor^® 790

HA-probe 抗体 (F-7) Alexa Fluor^® 405

I have an expression plasmid where the HA-taq is located like this: N´- 2xNLS - linker - HA - protein sequence -C´. Can this antibody detect the HA-tag when it is not N- or C-terminal, but in the middle of the protein sequence?