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GRP 94 Antibody (9G10) - Western Blotting - Image 380885
GRP 94 抗体 (9G10): sc-32249. HEL 92. 1. 7 (A), Hep G2 (B), c4 (C), 3T3-L1 (D), Neuro-2A (E) 和 C6 (F) 全细胞裂解液中 GRP 94 表达的 Western 印迹分析.
GRP 94 抗体 (9G10): sc-32249. HEL 92. 1. 7 (A), Hep G2 (B), c4 (C), 3T3-L1 (D), Neuro-2A (E) 和 C6 (F) 全细胞裂解液中 GRP 94 表达的 Western 印迹分析.

GRP 94 抗体 (9G10): sc-32249

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说明书
  • GRP 94 抗体 9G10 是大鼠单克隆 IgG2a，GRP 94抗体, 在32篇文献中引用，规格为200 µg/ml
  • 针对禽输卵管GRP 94
  • GRP 94 抗体 (9G10) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和chicken 来源的 GRP 94
  • 抗GRP 94抗体（9G10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GRP 94 (9G10): sc-32249的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成GRP 94 抗体 (9G10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
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研究信息

関連項目

此外 Glucose-Regulated Protein 抗体 包括 GRP 75, GRP 78和GRP 94

GRP 94抗体（9G10）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠、人类和鸡源的GRP 94蛋白。抗GRP 94抗体（9G10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。GRP 94蛋白，也称为热休克蛋白90kDa beta成员1（HSP90B1），作为位于内质网（ER）中的分子伴侣发挥着至关重要的作用，GRP 94协助蛋白质正确折叠和成熟，特别是那些参与细胞信号传导和免疫反应的蛋白质。GRP 94在ER中的定位确保了蛋白质在运送到最终目的地之前得到正确处理，这对于维持细胞内稳态和应对压力条件至关重要。GRP 94在癌症中尤为重要，因为GRP 94在肿瘤细胞中通常过度表达，有助于在不利条件下存活和增殖。GRP 94与多种蛋白质相互作用，包括肿瘤排斥抗原1（TRA1）和肿瘤坏死因子受体相关蛋白1（TRAP1），进一步凸显了GRP 94在细胞过程中的重要性以及作为治疗靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GRP 94 抗体 (9G10) 参考文献:

  1. PKC epsilon 是热休克反应中 hsp90 beta 基因的独特调节因子。  |  Wu, JM., et al. 2003. J Biol Chem. 278: 51143-9. PMID: 14532285
  2. HSP90 与癌症的伴侣作用。  |  Whitesell, L. and Lindquist, SL. 2005. Nat Rev Cancer. 5: 761-72. PMID: 16175177
  3. Hsp90 促进新性状的快速进化：多种真菌的抗药性。  |  Cowen, LE. and Lindquist, S. 2005. Science. 309: 2185-9. PMID: 16195452
  4. 通过可逆乙酰化调节分子伴侣 Hsp90 的功能。  |  Aoyagi, S. and Archer, TK. 2005. Trends Cell Biol. 15: 565-7. PMID: 16199163
  5. 人类基因组中的 HSP90 基因家族：对其分化和进化的见解。  |  Chen, B., et al. 2005. Genomics. 86: 627-37. PMID: 16269234
  6. Hsp90：蛋白质折叠和基因调控的伴侣。  |  Zhao, R. and Houry, WA. 2005. Biochem Cell Biol. 83: 703-10. PMID: 16333321
  7. 底物从伴侣 Hsp70 转移到 Hsp90。  |  Wegele, H., et al. 2006. J Mol Biol. 356: 802-11. PMID: 16403523
  8. 新生物素对 Hsp90 C 端结构域的伴侣功能和辅助伴侣相互作用的调节：香豆素类抗生素破坏 Hsp90 二聚化的证据。  |  Allan, RK., et al. 2006. J Biol Chem. 281: 7161-71. PMID: 16421106
  9. ATP 向粗面内质网衍生囊泡腔内的转运及其与包括 BiP（GRP 78）和 GRP 94 在内的腔内蛋白的结合。  |  Clairmont, CA., et al. 1992. J Biol Chem. 267: 3983-90. PMID: 1740446
  10. 癌症中的分子伴侣 GRP94/GP96： 肿瘤发生和治疗靶点。  |  Duan, X., et al. 2021. Front Oncol. 11: 629846. PMID: 33898309
  11. FBXL2 可抵消 Grp94，从而破坏表皮生长因子受体的稳定性，抑制表皮生长因子受体驱动的 NSCLC 生长。  |  Niu, M., et al. 2021. Nat Commun. 12: 5919. PMID: 34635651
  12. Grp 94相关蛋白对胰腺胆盐依赖性脂肪酶折叠和转运的伴侣功能。  |  Bruneau, N. and Lombardo, D. 1995. J Biol Chem. 270: 13524-33. PMID: 7768954

订购信息

产品名称产品编号规格价格数量收藏夹

GRP 94 抗体 (9G10)

sc-32249
200 µg/ml
$316.00

GRP 94 抗体 (9G10) AC

sc-32249 AC
500 µg/ml, 25% agarose
$416.00

GRP 94 抗体 (9G10) HRP

sc-32249 HRP
200 µg/ml
$316.00

GRP 94 抗体 (9G10) FITC

sc-32249 FITC
200 µg/ml
$330.00

GRP 94 抗体 (9G10) PE

sc-32249 PE
200 µg/ml
$343.00

GRP 94 抗体 (9G10) Alexa Fluor® 488

sc-32249 AF488
200 µg/ml
$357.00

GRP 94 抗体 (9G10) Alexa Fluor® 546

sc-32249 AF546
200 µg/ml
$357.00

GRP 94 抗体 (9G10) Alexa Fluor® 594

sc-32249 AF594
200 µg/ml
$357.00

GRP 94 抗体 (9G10) Alexa Fluor® 647

sc-32249 AF647
200 µg/ml
$357.00

GRP 94 抗体 (9G10) Alexa Fluor® 680

sc-32249 AF680
200 µg/ml
$357.00

GRP 94 抗体 (9G10) Alexa Fluor® 790

sc-32249 AF790
200 µg/ml
$357.00

Hi, What is the immunogen for this antibody? Which domain of GRP94? Thanks Andrew

Asked by: Andrew44444
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-07

Can GRP 94 (9G10): sc-32249 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, GRP 94 (9G10): sc-32249 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works very well in WB on human and murine cells.We have succesfully detected GRP-94 expression by western blot on lysates of human (Hek293) and murine (B16F10) cells, obtaining very clean signals. Antibody diluted at 1:150.
Date published: 2023-03-05
Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-24
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~94 kDa target protein in a HeLa/Jurkat cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from WB: works well in HeLaWB: works well in HeLa, K-562, NIH/3T3, KNRK, SK-BR-3 and T-47D whole cell lysates -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Clean bandsClean bands - immunoblotting of murine bone marrow mononuclear cells (BMMC). -SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by from HSP 90 expression observed in western blotHSP 90 expression observed in western blot analysis of non-transfected and human HSP 90 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-19
Rated 5 out of 5 by from Published western blot data using human foreskinPublished western blot data using human foreskin fibroblasts. -SCBT Publication Review
Date published: 2015-02-28
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GRP 94 抗体 (9G10) is rated 5.0 out of 5 by 8.
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