Date published: 2025-9-5
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Glutathione Peroxidase 4/GPX4 Antibody (E-12) - Western Blotting - Image 389015
GPx-4 抗体 (E-12) HRP: sc-166570 HRP. 对 MCF7 (A), F9 (B), AN3 CA (C) 和 Neuro-2A (D) 全细胞裂解液以及小鼠睾丸 (E) 和人类睾丸 (F) 组织提取物中 GPx-4 的表达进行直接 Western 印迹分析.
GPx-4 抗体 (E-12) HRP: sc-166570 HRP. 对 MCF7 (A), F9 (B), AN3 CA (C) 和 Neuro-2A (D) 全细胞裂解液以及小鼠睾丸 (E) 和人类睾丸 (F) 组织提取物中 GPx-4 的表达进行直接 Western 印迹分析.

Glutathione Peroxidase 4/GPX4 抗体 (E-12): sc-166570

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说明书
  • Glutathione Peroxidase 4/GPX4 抗体 E-12 是小鼠单克隆 IgG2b κ，Glutathione Peroxidase 4/GPX4抗体, 在87篇文献中引用，规格为200 µg/ml
  • 针对氨基酸108-197的抗原，在GPx-4的C-terminus处映射，human来源
  • Glutathione Peroxidase 4/GPX4 抗体 (E-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GPx-4
  • 抗Glutathione Peroxidase 4/GPX4抗体（E-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Glutathione Peroxidase 4/GPX4 (E-12): sc-166570的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Glutathione Peroxidase 4/GPX4 抗体 (E-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Glutathione Peroxidase 4/GPX4 抗体 (E-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外GPx抗体，包括GPx-1, GPx-2, GPx-3, GPx-4和GPx-5

GPx-4抗体（E-12）是一种IgG2b κ型小鼠单克隆GPx-4抗体（也被称为GPX4抗体、PHGPx抗体、磷脂氢过氧化物谷胱甘肽过氧化物酶抗体、磷脂氢过氧化物酶抗体、SnPHGPx抗体、SnGPx抗体或SMDS抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组化（IHC-P）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的GPx-4蛋白。GPx-4抗体（E-12）既可作为非共轭的抗GPx-4抗体形式提供，也可作为多种共轭形式的抗GPx-4抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。谷胱甘肽过氧化物酶（GPx）**酶通常是含硒的四聚体糖蛋白，有助于防止细胞膜的脂质过氧化。GPx酶能将脂质氢过氧化物还原为醇，并将游离的过氧化氢还原为水。在高等脊椎动物中，GPx成员是少数已知含有硒代半胱氨酸的蛋白质之一，这种硒代半胱氨酸位于谷胱甘肽过氧化物酶的活性部位，并由无义（终止）密码子TGA编码。目前已知有八种GPx同源物（GPx-1至GPx-8）。其中，GPx-1、GPx-2和GPx-3以同源四聚体的形式存在。GPx-4则具有高形成高分子量寡聚体的倾向。GPx-1在应对氧化应激时，对血管壁和神经细胞的抗氧化防御起着重要作用。GPx-2是肺部的主要同工型，其基础或诱导表达依赖于Nrf2。GPx-3受低氧应激的调节，其表达和缺乏与心血管疾病和中风有关。与其他成员不同，GPx-5不依赖于硒，它与精子的头部结合，在那里它可以保护精子在附睾中不发生过早的顶体反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glutathione Peroxidase 4/GPX4 抗体 (E-12) 参考文献:

  1. GPX4 对硒的利用是防止过氧化氢诱导的铁猝死的必要条件  |  Ingold, I., et al. 2018. Cell. 172: 409-422.e21. PMID: 29290465
  2. GPX4 在铁氧化过程中的作用及其药理作用  |  Seibt, TM., et al. 2019. Free Radic Biol Med. 133: 144-152. PMID: 30219704
  3. 在 AKI 中，Legumain 可通过促进伴侣介导的 GPX4 自噬来促进肾小管铁变态反应。  |  Chen, C., et al. 2021. Cell Death Dis. 12: 65. PMID: 33431801
  4. Wnt/beta-catenin信号通过靶向GPX4赋予胃癌铁蛋白酶抗性  |  Wang, Y., et al. 2022. Cell Death Differ. 29: 2190-2202. PMID: 35534546
  5. 硫司群通过 STAT3/GPX4 信号诱导胰腺癌细胞的铁变态反应  |  Zhang, W., et al. 2022. Cell Death Dis. 13: 630. PMID: 35859150
  6. 黄芩苷通过新型Nrf2/xCT/GPX4调控轴诱导骨肉瘤中的铁细胞减少症  |  Wen, RJ., et al. 2023. Phytomedicine. 116: 154881. PMID: 37209607
  7. HDAC3 畸变导致的 GPX4 抑制推动了肾脏铁变态反应和 AKI-CKD 进展。  |  Zhang, L., et al. 2023. Redox Biol. 68: 102939. PMID: 37890360
  8. SMURF2通过促进GSTP1降解，以GPX4无关的方式使癌细胞易于发生铁变态反应。  |  Zhang, W., et al. 2023. Mol Cell. 83: 4352-4369.e8. PMID: 38016474
  9. GPX4 的 m6A 和 m5C 修饰可通过 STING 激活促进抗癌免疫。  |  Chen, B., et al. 2023. Cell Death Dis. 14: 809. PMID: 38065948
  10. OTUD5的自噬作用会破坏GPX4的稳定性，从而导致依赖于铁蛋白沉积的肾损伤。  |  Chu, LK., et al. 2023. Nat Commun. 14: 8393. PMID: 38110369

订购信息

产品名称产品编号规格价格数量收藏夹

Glutathione Peroxidase 4/GPX4 抗体 (E-12)

sc-166570
200 µg/ml
$316.00

Glutathione Peroxidase 4/GPX4 (E-12): m-IgGκ BP-HRP 套装

sc-521659
200 µg Ab, 40 µg BP
$354.00

Glutathione Peroxidase 4/GPX4 (E-12): m-IgG2b BP-HRP 套装

sc-548948
200 µg Ab; 10 µg BP
$354.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) AC

sc-166570 AC
500 µg/ml, 25% agarose
$416.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) HRP

sc-166570 HRP
200 µg/ml
$316.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) FITC

sc-166570 FITC
200 µg/ml
$330.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) PE

sc-166570 PE
200 µg/ml
$343.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) Alexa Fluor® 488

sc-166570 AF488
200 µg/ml
$357.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) Alexa Fluor® 546

sc-166570 AF546
200 µg/ml
$357.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) Alexa Fluor® 594

sc-166570 AF594
200 µg/ml
$357.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) Alexa Fluor® 647

sc-166570 AF647
200 µg/ml
$357.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) Alexa Fluor® 680

sc-166570 AF680
200 µg/ml
$357.00

Glutathione Peroxidase 4/GPX4 抗体 (E-12) Alexa Fluor® 790

sc-166570 AF790
200 µg/ml
$357.00

Is the protein sequence of this antibody provided somewhere? Or will it be provided once purchased? 

Asked by: vs2000
Thank you for your question. Glutathione Peroxidase 4/GPX4 Antibody (E-12): sc-166570 was raised against amino acids 108-197 mapping at the C-terminus of GPx-4 of human origin.
Answered by: Tech Support Europe
Date published: 2022-10-25

Hello Which is the recomended protocol for cell fixation and permeabilization to be used in flow cytometry? And the recomended staining dilution?

Asked by: Rsilva
Thank you for your question. For cell fixation and permeabilization, we recommend using our FCM Fixation Buffer (sc-3622) and FCM Permeabilization Buffer, sc-3623. We suggest 1 µg of fluorochrome-conjugated antibody per 100 µl of the prepared cell suspension (equal to 1 million cells). Please find our the link to our FCM protocol below. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: Blake J
Date published: 2021-07-08

What is the difference between GPX-4 E-12 and B-12? E-12 is suitable for SHSY-5Y cells?

Asked by: chateauxpers
Thank you for your question. These antibodies have been raised against the same amino acid range but bind to different epitopes within that region. While GPx-4 Antibody (B-12) is not recommended for IHC/IHC(P) use, both antibodies are suitable for the detection of GPx-4 in any cells or tissues of mouse, rat or human origin by WB, IP, IF, and ELISA.
Answered by: Tech Support Europe
Date published: 2025-03-28

Hi there. I have some questions for the antibody, GPx-4 (E-12) sc-166570 . Should this antibody be made in a 5% skim milk blocking solution in PBST? Or should a different blocking solution be used? Similarly, should washing steps be performed in PBST?

Asked by: GuyFenton
Thank you for your question. We recommend using 5% nonfat milk in TBST for blocking and for primary and secondary antibody incubations. We recommend washing in TBST, with a final wash in TBS without Tween-20. Please refer to our Western blotting protocol for more detailed information.
Answered by: Technical Support
Date published: 2019-03-10

What is the recommended starting dilution for Western blot for sc-166570: GPx-4 (E-12) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from 抗体特异性特别强我们在实验中应用了这个抗体，我们发现这个抗体特异性特别强，灵敏度很高，背景噪声低，显色特别清楚，拍摄的照片很漂亮，是实验的理想选择
Date published: 2025-04-10
Rated 1 out of 5 by from Not good for IHCi tried to perform IHC with this ab. I either tried with different antigen retrival buffer or change the dilution, but i didnt get any positivity
Date published: 2024-05-23
Rated 4 out of 5 by from It works wellWe used this antibody in WB, good signal. 1:500 concentration
Date published: 2023-10-23
Rated 4 out of 5 by from Buon anticorpo per wbL'anticorpo è stato testato su cellule U373. Ottimi segnali
Date published: 2021-09-28
Rated 1 out of 5 by from not very wellnot recommended for WB in 293T and HT1080 cells. Very weak and not clean signal. I tried two different dilutions (1:500 and 1:100) but the signal is always too weak and dirty.
Date published: 2021-08-09
Rated 5 out of 5 by from はっきりみえたウェスタンブロッティングで使用させていただきました。バンドが、予想される位置にはっきりと見えたので非常に良い商品だと感じました。　ありがとうございます。　
Date published: 2021-06-21
Rated 3 out of 5 by from Good antibody for WBThis antibody worked very well with mouse tissue for WB
Date published: 2018-10-03
Rated 4 out of 5 by from Worked in HCC827 cellsWorked at 1:500 concentration for western blot; used HCC827 cancer cell line. Faint band, but readable and reliable!
Date published: 2018-04-18
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Glutathione Peroxidase 4/GPX4 抗体 (E-12) is rated 4.0 out of 5 by 22.
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