Date published: 2025-9-9
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gp91phox/CYBB/NOX2 Antibody (54.1) - Immunohistochemistry - Image 353005
gp91-phox 抗体 (54. 1): sc-130543. 福尔马林固定, 石蜡包埋的人骨髓组织的免疫过氧化物酶染色显示造血细胞的胞浆染色.
gp91-phox 抗体 (54. 1): sc-130543. 福尔马林固定, 石蜡包埋的人骨髓组织的免疫过氧化物酶染色显示造血细胞的胞浆染色.

gp91phox/CYBB/NOX2 抗体 (54.1): sc-130543

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说明书
  • gp91phox/CYBB/NOX2 抗体 54.1 是小鼠单克隆 IgG1 κ，gp91phox/CYBB/NOX2抗体, 在146篇文献中引用，规格为200 µg/ml
  • 与human的gp91-phox相比，具有与氨基酸383-390的表位图谱
  • gp91phox/CYBB/NOX2 抗体 (54.1) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 gp91-phox; 还建议用于检测ERp57, Mox1, Nox3 and Nox4
  • 抗gp91phox/CYBB/NOX2抗体（54.1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取gp91phox/CYBB/NOX2 (54.1): sc-130543的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 gp91phox/CYBB/NOX2 抗体 (54.1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与gp91phox/CYBB/NOX2 抗体 (54.1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

gp91-phox抗体（54.1）是一种IgG1 κ小鼠单克隆gp91-phox抗体（也称为CYBB抗体、细胞色素b-245 beta链抗体或GP91PHOX抗体），可通过WB、IP、IF和IHC(P)检测小鼠、大鼠和人源的gp91-phox蛋白。gp91-phox抗体（54.1）既可以是非偶联的抗gp91-phox抗体形式，也可以是多种偶联形式的抗gp91-phox抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Mox1和糖蛋白gp91-phox是密切相关的蛋白质，它们是NADPH氧化酶的重要组成部分。产生超氧化物的NADPH氧化酶存在于吞噬细胞、神经上皮体、血管平滑肌细胞和内皮细胞中。它包括一个膜结合的黄素细胞色素，该细胞色素含有两个亚基gp91-phox和p22-phox，以及胞质蛋白p47-phox和p67-phox。在NADPH氧化酶激活过程中，p47-phox和p67-phox迁移到质膜，在那里它们与黄素细胞色素b558结合，形成有活性的酶复合物。p22-和gp91-phox亚基还作为表面O2传感器，在缺氧条件下启动细胞信号传导。Mox1和gp91具有相同的C末端序列，但它们的表达模式不同。gp91-phox在嗜酸性粒细胞、中性粒细胞、单核细胞和B淋巴细胞中表达，而Mox1主要在结肠中检测到，在子宫和前列腺中也有低表达。Mox1在血管平滑肌细胞中对PDGF刺激也有上调反应，这共同表明Mox1可能与gp91-phox有类似的功能，但在非吞噬细胞中调节NADPH超氧化物的产生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

gp91phox/CYBB/NOX2 抗体 (54.1) 参考文献:

  1. 缺乏 NADPH 氧化酶 gp91 phox 亚基的小鼠对 O2 的感应得以保留。  |  Archer, SL., et al. 1999. Proc Natl Acad Sci U S A. 96: 7944-9. PMID: 10393927
  2. 产生超氧化物的氧化酶 Mox1 对细胞的改造  |  Suh, YA., et al. 1999. Nature. 401: 79-82. PMID: 10485709
  3. 严重恶性骨质疏松症患者转化的 B 淋巴细胞中超氧化物的生成。  |  Yang, S., et al. 1999. Mol Cell Biochem. 199: 15-24. PMID: 10544947
  4. 人类内皮细胞中功能性白细胞型 NADPH 氧化酶的鉴定：潜在的致动脉粥样硬化活性氧来源。  |  Meyer, JW., et al. 1999. Endothelium. 7: 11-22. PMID: 10599557
  5. 证据表明，在人类多形核白细胞中，不同的信号转导通路参与调节NADPH氧化酶成分（gp91-phox、p47-phox和p22-phox）和IgG高亲和力受体（Fc gamma R-I）的组成和干扰素γ依赖性基因表达。  |  Amezaga, MA., et al. 1992. Blood. 79: 735-44. PMID: 1531037
  6. Prodigiosin抑制gp91(phox)和iNOS的表达，保护小鼠免受缺氧缺血性脑损伤。  |  Chang, CC., et al. 2011. Toxicol Appl Pharmacol. 257: 137-47. PMID: 21925195
  7. 单核细胞AML细胞灭活抗白血病淋巴细胞：NADPH氧化酶/gp91（phox）表达和PARP-1/PAR凋亡途径的作用  |  Aurelius, J., et al. 2012. Blood. 119: 5832-7. PMID: 22550344
  8. 基于AAV的RNAi沉默NADPH氧化酶gp91（phox）可减轻寒冷引起的心血管功能障碍。  |  Wang, X., et al. 2012. Hum Gene Ther. 23: 1016-26. PMID: 22888847
  9. 在顺铂治疗的大鼠肾小球和近端小管中，超氧阴离子产生以及gp91(phox)和p47(phox)的表达增加。  |  Trujillo, J., et al. 2015. J Biochem Mol Toxicol. 29: 149-56. PMID: 25388649
  10. 白藜芦醇通过上调U937细胞中gp91-phox基因的表达，显著增强视黄酸诱导的超氧化物生成活性。  |  Kikuchi, H., et al. 2018. Biochem Biophys Res Commun. 495: 1195-1200. PMID: 29183727
  11. 花生四烯酸可激活的 NADPH 氧化酶缔合作用 H+ 通道。有证据表明，gp91-phox 是该通道的重要组成部分。  |  Henderson, LM., et al. 1995. J Biol Chem. 270: 5909-16. PMID: 7890722
  12. p22phox 是产生超氧化物的 NADH/NADPH 氧化酶系统的重要组成部分，它调节血管紧张素 II 诱导的血管平滑肌细胞肥大。  |  Ushio-Fukai, M., et al. 1996. J Biol Chem. 271: 23317-21. PMID: 8798532

订购信息

产品名称产品编号规格价格数量收藏夹

gp91phox/CYBB/NOX2 抗体 (54.1)

sc-130543
200 µg/ml
$316.00

gp91phox/CYBB/NOX2 (54.1): m-IgG Fc BP-HRP 套装

sc-528733
200 µg Ab; 10 µg BP
$354.00

gp91phox/CYBB/NOX2 (54.1): m-IgGκ BP-HRP 套装

sc-521213
200 µg Ab, 40 µg BP
$354.00

gp91phox/CYBB/NOX2 (54.1): m-IgG1 BP-HRP 套装

sc-543101
200 µg Ab; 20 µg BP
$354.00

gp91phox/CYBB/NOX2 抗体 (54.1) AC

sc-130543 AC
500 µg/ml, 25% agarose
$416.00

gp91phox/CYBB/NOX2 抗体 (54.1) HRP

sc-130543 HRP
200 µg/ml
$316.00

gp91phox/CYBB/NOX2 抗体 (54.1) FITC

sc-130543 FITC
200 µg/ml
$330.00

gp91phox/CYBB/NOX2 抗体 (54.1) PE

sc-130543 PE
200 µg/ml
$343.00

gp91phox/CYBB/NOX2 抗体 (54.1) Alexa Fluor® 488

sc-130543 AF488
200 µg/ml
$357.00

gp91phox/CYBB/NOX2 抗体 (54.1) Alexa Fluor® 546

sc-130543 AF546
200 µg/ml
$357.00

gp91phox/CYBB/NOX2 抗体 (54.1) Alexa Fluor® 594

sc-130543 AF594
200 µg/ml
$357.00

gp91phox/CYBB/NOX2 抗体 (54.1) Alexa Fluor® 647

sc-130543 AF647
200 µg/ml
$357.00

gp91phox/CYBB/NOX2 抗体 (54.1) Alexa Fluor® 680

sc-130543 AF680
200 µg/ml
$357.00

gp91phox/CYBB/NOX2 抗体 (54.1) Alexa Fluor® 790

sc-130543 AF790
200 µg/ml
$357.00

è possibile usare questo anticorpo per IP?

Asked by: Mavi
Thank you for your question. gp91phox/CYBB/NOX2 Antibody (54.1) is recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF and IHC(P). It is also recommended for detection of ERp57, Mox1, Nox3 and Nox4 gp91phox/CYBB/NOX2 Antibody (54.1) é raccomandato per il rilevamento di gp91-phox di origine mouse, rat e human in WB, IP, IF e IHC(P); anche raccomandato per riconoscimento di ERp57, Mox1, Nox3 and Nox4.
Answered by: Tech Support Europe
Date published: 2024-12-06

Does this antibody also detect in mice tissue all other Nox isoforms besides Nox2? your datasheet explains it is good for nox3 and nox4 detection too. Does it mean the signal in the whole brain is an overall nox2+nox3 and 4?

Asked by: Aurora2023
Thank you for your question. gp91-phox (54.1) antibody is recommended for ERp57, Mox1, Nox3 and Nox4. We recommend using mouse, rat or human samples .
Answered by: BlakeJ
Date published: 2022-12-15

Can this antibody be used for frozen tissue (for IHC)?

Asked by: mart7
Thank you for your question. Anti-gp91-phox Antibody (54.1): sc-130543 is recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF as well as IHC on paraffin-embedded and frozen tissue sections.
Answered by: Tech Support Europe
Date published: 2022-06-21

Does this antibody work with flow cytometry?

Asked by: pinder
thank you for your question. Anti-gp91-phox Antibody (54.1) is available conjugated to either phycoerythrin or FITC for FCM, IF, and IHC(P).
Answered by: Tech Support Europe
Date published: 2021-04-07

Can I use this antibody conjugated with Phycoerythrin for IHC or IF? If yes, under which channel can I image the stained cells? Is there a protocol for IHC or IF for this antibody? Thank you

Asked by: Keertana
Thank you for your question. Anti-gp91-phox Antibody (54.1) and its conjugates are recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF and IHC(P). They are also recommended for detection of ERp57, Mox1, Nox3 and Nox4. You can find our recommended protocols for IHC and IF experiments under this link: https://www.scbt.com/resources/protocols PE absorption peaks in the visible light spectrum are measured at 495 and 545/566 nm.
Answered by: Technical Support Europe
Date published: 2020-10-12

Is it possible for me to have a free trial sample for the above antibody? Thank you!

Asked by: Renato
Thank you very much for your inquiry. Please send us an email to uk@scbt.com with your complete delivery address, we will contact you back as soon as possible.
Answered by: Technical Support Europe
Date published: 2017-12-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-130543 PE) or fluoroscein (sc-130543 FITC), Alexa Fluor® 488 (sc-130543 AF488) or Alexa Fluor® 647 (sc-130543 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-04
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Rated 4 out of 5 by from Buon funzionamentoBuon funzionamento anche se qualche banda aspecifica. Dil 1:100
Date published: 2025-05-30
Rated 5 out of 5 by from Good antibody for Western blotIt works great - I'd recommend this Nox2 antibody.
Date published: 2022-05-05
Rated 5 out of 5 by from Good antibody for Western blotProduced specific Western blot data of NOX2 expression in mouse mesenteric arteries extracts.
Date published: 2022-02-09
Rated 5 out of 5 by from good antibodythe antibody works very well in IHC. No problem with background.
Date published: 2020-12-01
Rated 5 out of 5 by from Very good for Western BlottingWorks great for western blotting (mouse heart lysate), can see clear bands at about 60 kDa.
Date published: 2019-07-05
Rated 5 out of 5 by from workedIt works great - I 'd recommend this Nox2 antibody.
Date published: 2018-03-16
Rated 5 out of 5 by from Great IB antibodyThis antibody works great with mouse cells. We also included Nox2-KO cells as a genetic control.
Date published: 2017-10-08
Rated 4 out of 5 by from very goodThe strips are very clear and there are not many bands.
Date published: 2017-08-27
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gp91phox/CYBB/NOX2 抗体 (54.1) is rated 4.9 out of 5 by 23.
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