Date published: 2025-9-6
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Glucose Transporter Glut3 Antibody (G-5) - Western Blotting - Image 376845
Glut3 抗体 (G-5) HRP: sc-74399 HRP. Hep G2 (A), ES-2 (B) 和 TE671 (C) 全细胞裂解液中 Glut3 表达的直接 Western 印迹分析.
Glut3 抗体 (G-5) HRP: sc-74399 HRP. Hep G2 (A), ES-2 (B) 和 TE671 (C) 全细胞裂解液中 Glut3 表达的直接 Western 印迹分析.

Glucose Transporter Glut3 抗体 (G-5): sc-74399

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寫評論提問

说明书
  • Glucose Transporter Glut3 抗体 G-5 是小鼠单克隆 IgG1 κ，Glucose Transporter Glut3抗体, 在42篇文献中引用，规格为200 µg/ml
  • 针对氨基酸216-265的图谱，在Glut3的内部区域，human起源
  • Glucose Transporter Glut3 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Glut3
  • 抗Glucose Transporter Glut3抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Glucose Transporter Glut3 (G-5): sc-74399的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Glucose Transporter Glut3 Antibody (G-5) for WB applications. 的首选辅助检测试剂，这些试剂现与Glucose Transporter Glut3 Antibody (G-5) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Glut抗体，包括Glut1, Glut10, Glut2, Glut3, Glut4, Glut5和Glut6

Glut3抗体（G-5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Glut3蛋白。抗 -Glut3抗体（G-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Glut3，也称为SLC2A3，在葡萄糖转运中起着关键作用，尤其是在大脑中，Glut3主要在大脑中表达。这种高亲和力的葡萄糖转运体对于维持神经组织中的葡萄糖稳态至关重要，因为神经元严重依赖葡萄糖作为其主要能量来源。Glut3促进葡萄糖快速摄取的能力对于支持大脑的高代谢需求至关重要，尤其是在活动增加或压力大的时期。了解Glut3的功能至关重要，因为葡萄糖转运失调会导致各种神经疾病和代谢疾病。在研究中使用Glut3 (G-5)抗体可以深入了解葡萄糖转运机制及其对健康和疾病的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glucose Transporter Glut3 抗体 (G-5) 参考文献:

  1. 葡萄糖转运体同工型在非极化哺乳动物细胞中的差异定位：GLUT1 而非 GLUT3 分布于抗清洁剂膜域。  |  Sakyo, T. and Kitagawa, T. 2002. Biochim Biophys Acta. 1567: 165-75. PMID: 12488050
  2. 葡萄糖剥夺和 IGF-I 对绒毛膜细胞中葡萄糖转运和 GLUT1/GLUT3 转运体的不同调控。  |  Fladeby, C., et al. 2003. Biochim Biophys Acta. 1593: 201-8. PMID: 12581864
  3. PC12 细胞的葡萄糖摄取：新型 GLUT3-GFP 融合蛋白揭示的 GLUT3 囊泡贩运和融合。  |  Heather West Greenlee, M., et al. 2003. J Neurosci Res. 73: 518-25. PMID: 12898536
  4. 一氧化氮抑制线粒体呼吸可通过 5'-AMP 激活蛋白激酶迅速刺激细胞保护 GLUT3 介导的葡萄糖摄取。  |  Cidad, P., et al. 2004. Biochem J. 384: 629-36. PMID: 15320870
  5. 3T3-L1 脂肪细胞中 GLUT-4 靶向的分子调控。  |  Marsh, BJ., et al. 1995. J Cell Biol. 130: 1081-91. PMID: 7544796
  6. 培养的大鼠脂肪细胞中葡萄糖转运体的调控：胰岛素和地塞米松通过启动子激活对 GLUT4 基因表达的协同作用。  |  Hajduch, E., et al. 1995. Endocrinology. 136: 4782-9. PMID: 7588207
  7. 大鼠前脑整体缺血后，神经元特异性葡萄糖转运体 GLUT3 在海马中的免疫反应性逐渐丧失。  |  McCall, AL., et al. 1995. Brain Res. 670: 29-38. PMID: 7719721
  8. 洞穴素在脂肪细胞中的表达和分区：与 GLUT4 的协调调控和结构分离。  |  Kandror, KV., et al. 1995. J Cell Biol. 129: 999-1006. PMID: 7744970
  9. 下丘脑 GLUT 4 的表达：葡萄糖和胰岛素感应机制？  |  Livingstone, C., et al. 1995. Mol Cell Endocrinol. 107: 67-70. PMID: 7796936
  10. 促进葡萄糖转运体  |  Mueckler, M. 1994. Eur J Biochem. 219: 713-25. PMID: 8112322
  11. 葡萄糖转运体在肠道, 肾脏和肝脏葡萄糖通量调节中的作用。  |  Thorens, B. 1996. Am J Physiol. 270: G541-53. PMID: 8928783

订购信息

产品名称产品编号规格价格数量收藏夹

Glucose Transporter Glut3 抗体 (G-5)

sc-74399
200 µg/ml
$316.00

Glucose Transporter Glut3 (G-5): m-IgG Fc BP-HRP 套装

sc-527016
200 µg Ab; 10 µg BP
$354.00

Glucose Transporter Glut3 (G-5): m-IgG1 BP-HRP 套装

sc-532389
200 µg Ab; 20 µg BP
$354.00

Glucose Transporter Glut3 抗体 (G-5) AC

sc-74399 AC
500 µg/ml, 25% agarose
$416.00

Glucose Transporter Glut3 抗体 (G-5) HRP

sc-74399 HRP
200 µg/ml
$316.00

Glucose Transporter Glut3 抗体 (G-5) FITC

sc-74399 FITC
200 µg/ml
$330.00

Glucose Transporter Glut3 抗体 (G-5) PE

sc-74399 PE
200 µg/ml
$343.00

Glucose Transporter Glut3 抗体 (G-5) Alexa Fluor® 488

sc-74399 AF488
200 µg/ml
$357.00

Glucose Transporter Glut3 抗体 (G-5) Alexa Fluor® 546

sc-74399 AF546
200 µg/ml
$357.00

Glucose Transporter Glut3 抗体 (G-5) Alexa Fluor® 594

sc-74399 AF594
200 µg/ml
$357.00

Glucose Transporter Glut3 抗体 (G-5) Alexa Fluor® 647

sc-74399 AF647
200 µg/ml
$357.00

Glucose Transporter Glut3 抗体 (G-5) Alexa Fluor® 680

sc-74399 AF680
200 µg/ml
$357.00

Glucose Transporter Glut3 抗体 (G-5) Alexa Fluor® 790

sc-74399 AF790
200 µg/ml
$357.00

Hi, I have recently purchased Glucose Transporter Glut3 Antibody (G-5) Alexa Fluor® 488 sc-74399 AF488 , was wondering if my antibody will work for both mouse and human samples. Regards, Shubham

Asked by: Shubham
Thank you for your question. Glucose Transporter Glut3 Antibody (G-5) is recommended for detection of Glut3 of mouse, rat and human origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2025-04-03

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from Perfect antibodyI got the trail size of this antibody and I got nice protein bands in brain samples.
Date published: 2018-05-22
Rated 1 out of 5 by from kein Ergebnis im WBhat im WB (1/200) mit SHSY5Y + HEP Zellen keine spezifischen Banden gezeigt
Date published: 2018-04-03
Rated 5 out of 5 by from Very goodWorks very well in human neural stem cells. Visible band at 55 kDa.
Date published: 2017-12-14
Rated 5 out of 5 by from Strong WB and IFGreat western data; strong signal, low background; also very nice IF staining results
Date published: 2017-01-27
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-16
Rated 5 out of 5 by from Definite band of correct molecular weightDefinite band of correct molecular weight for Glut3 observed by Western Blot using U-87 MG whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Major band at expected molecular weightMajor band at expected molecular weight, with faint bands below observed by Western Blot for mouse brain tissue extract. -SCBT QC
Date published: 2015-04-11
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Glucose Transporter Glut3 抗体 (G-5) is rated 4.6 out of 5 by 12.
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