GLI-1/GLI1抗体 (C-1) 推荐用于 mouse, rat 和 human 来源的GLI-1 WB, IP, IF 和 ELISA检测

GLI-1/GLI1 抗体 (C-1): sc-515751

GLI-1/GLI1抗体 (C-1) is rated 4.3 out of 5 by 12.
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说明书

    • GLI-1/GLI1抗体(C-1)是小鼠单克隆IgG1 κ, 在21篇文献中引用,规格为200 µg/ml
    • 免疫human物种的的氨基酸781-1080
    • 抗-GLI-1/GLI1 抗体 (C-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 GLI-1
    • 抗-GLI-1/GLI1 抗体 (C-1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-515751 X, 200 µg/0.1 ml)
    • 关于如何获取GLI-1/GLI1 (C-1): sc-515751免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 GLI-1/GLI1 Antibody (C-1) for WB 的首选应用二抗。这些试剂现在以套装形式提供GLI-1/GLI1 Antibody (C-1)。 (见下方订购信息).
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Has it been tested for human IHC(P) yet? If it isn't, is there any Santa Cruz anti-Gli1 validated for IHC(P)? I have searched for the antibody you recommended two years ago but it seems it's not available anymore. Thank you very much

Asked by: Pablo19
Thank you for your question. We have not yet tested this antibody on formalin-fixed paraffin-embedded tissue sections and so IHC(P) is not a recommended application at this time. We do currently offer an alternative antibody that has been tested for human IHC(P), GLI-1 Antibody (A-7): sc-515781.
Answered by: Technical Support
Date published: 2019-02-27

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515751: GLI-1 (C-1) mouse monoclonal antibody will work?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06

Are they good for human IHC(P)? Thank you.

Asked by: Natalia11
Thank you for your question. We have not yet tested our, GLI-1 Antibody (C-1): sc-515751, on formalin-fixed paraffin-embedded tissue sections, so we do not know if it is suitable for IHC(P). We do currently warranty the antibody to work for IF, or immunohistochemistry or IF on fresh/frozen tissues/cells fixed in methanol or acetone. We do offer an alternative antibody to GLI-1 that is validated to work for IHC(P) on human tissues, GLI-1 Antibody (1B9F8): sc-517290 (https://www.scbt.com/scbt/product/gli-1-antibody-1b9f8)
Answered by: Technical Support
Date published: 2017-02-03
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Rated 5 out of 5 by from good signal We used this antibody to investigate the expression and clinical significance of Gli1 protein in breast cancer tissues in Hedgehog signaling pathway,we found this antibody is very useful ,we got strong signal around 135kda use the dilution 1:800.
Date published: 2017-08-12
Rated 5 out of 5 by from Good antibody Using the concentration at 1:800/1:1000 for western blot is very good,
Date published: 2017-08-09
Rated 5 out of 5 by from very good with WB We got the sample for this antibody and it worked excellent for Western Blot! Strong Signal and clean background! We used human lung cancer cell lines PC9 A549 and H1299.
Date published: 2017-07-06
Rated 5 out of 5 by from great quality Works very well for western blotting; strong signal with no background; great antibody.
Date published: 2017-05-27
Rated 5 out of 5 by from Best one we've used. We've used other Gli1 antibodies with mediocre results- inconsistent banding, short degradation time of the antibody, poor signal. This Gli1, with the corresponding m-IgGk BP-HRP secondary gave us great and repeatable Western Blot results on a sometimes tricky project. See the basic WB data below where a tagged Gli1 was over expressed in a cell line (first 3 lanes) and empty vector controls (last 3 lines).
Date published: 2017-03-16
Rated 5 out of 5 by from the function of this antibody is very good I had tried Gli-1 antibody that comes from other company before I tried this antibody. I found that this antibody is very perfect
Date published: 2017-02-28
Rated 5 out of 5 by from no complaints I use this antibody do western blot at 1:500 dilution, in the F9 whole cell lysate the band is specific.
Date published: 2017-02-12
Rated 3 out of 5 by from oxygenation reactions. It is well known that zinc finger proteins include some form of transcriptional activation or inhibition that interacts with oxidases in the human body. Our experiments are performed by recruiting histone-modified proteins for oxygenation reactions.
Date published: 2017-02-12
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