GHS-R1 Antibody (E-7): sc-374515

GHS-R1 Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects GHS-R1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GHS-R1 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GHS-R1, also known as growth hormone secretagogue receptor type 1, is a G protein-coupled receptor that plays a crucial role in regulating growth hormone release. GHS-R1 binds to synthetic peptidyl and nonpeptidyl growth hormone secretagogues, which are essential for stimulating growth hormone secretion. Notably, the interaction between GHS and GHS-R1 is magnesium-dependent and is distinct from the regulation of growth hormone by hypothalamic hormones such as growth hormone-releasing hormone and somatostatin. This specificity indicates that GHS-R1 may serve as a unique regulator of growth hormone, highlighting potential importance in understanding growth disorders and metabolic diseases. GHS-R1 is primarily localized in the hypothalamus and pituitary gland, with elevated expression observed in pituitary adenoma tissues, suggesting involvement in pathological conditions related to growth hormone dysregulation.

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GHS-R1 Antibody (E-7) References:

1. Expression of functional growth hormone secretagogue receptors in human pituitary adenomas: polymerase chain reaction, triple in-situ hybridization and cell culture studies.  |  Barlier, A., et al. 1999. J Neuroendocrinol. 11: 491-502. PMID: 10444306
2. Identification, characterization, and biological activity of specific receptors for natural (ghrelin) and synthetic growth hormone secretagogues and analogs in human breast carcinomas and cell lines.  |  Cassoni, P., et al. 2001. J Clin Endocrinol Metab. 86: 1738-45. PMID: 11297611
3. GH-releasing peptides and bone.  |  Cocchi, D., et al. 2005. J Endocrinol Invest. 28: 11-4. PMID: 16323823
4. Ghrelin stimulates insulin-induced glucose uptake in adipocytes.  |  Patel, AD., et al. 2006. Regul Pept. 134: 17-22. PMID: 16338009
5. Association of cord blood des-acyl ghrelin with birth weight, and placental GHS-R1 receptor expression in SGA, AGA, and LGA newborns.  |  González-Domínguez, MI., et al. 2016. Endocrine. 53: 182-91. PMID: 26754660
6. Ghrelin receptor antagonist attenuated sickness behavior and activation of HPA-axis induced by immunological challenge in male rats.  |  Paula, DE., et al. 2022. Life Sci. 288: 120173. PMID: 34822799
7. Desacylghrelin modulates GHS-R1 receptor expression and cell differentiation in placental BeWo cells.  |  Coria-Caballero, V., et al. 2023. Mol Cell Endocrinol. 577: 112035. PMID: 37506870
8. Identification of a new G-protein-linked receptor for growth hormone secretagogues.  |  Pong, SS., et al. 1996. Mol Endocrinol. 10: 57-61. PMID: 8838145
9. Hypothalamic growth hormone secretagogue-receptor (GHS-R) expression is regulated by growth hormone in the rat.  |  Bennett, PA., et al. 1997. Endocrinology. 138: 4552-7. PMID: 9348177
10. Distribution of mRNA encoding the growth hormone secretagogue receptor in brain and peripheral tissues.  |  Guan, XM., et al. 1997. Brain Res Mol Brain Res. 48: 23-9. PMID: 9379845
11. Expression of the growth hormone secretagogue receptor in pituitary adenomas and other neuroendocrine tumors.  |  Korbonits, M., et al. 1998. J Clin Endocrinol Metab. 83: 3624-30. PMID: 9768675
12. Growth hormone-dependent regulation of pituitary GH secretagogue receptor (GHS-R) mRNA levels in the spontaneous dwarf Rat.  |  Kamegai, J., et al. 1998. Neuroendocrinology. 68: 312-8. PMID: 9822798

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GHS-R1 Antibody (E-7) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-GHS-R1 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GHS-R1 Antibody (E-7) Specifications

Alternate Product Name: Ghrelin Receptor

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 186-265

Epitope Target: GHS-R1

Epitope Species: human

Product Reactivity/Detection: GHS-R1a and GHS-R1b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for GHS-R1 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin