Date published: 2025-9-6
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GDF-15 Antibody (H-2) - Western Blotting - Image 356257
GDF-15 抗体 (H-2): sc-515675. LNCaP (A), C6 (B) 和 RIN-m5F (C) 全细胞裂解液及小鼠胎盘组织提取物 (D) 中 GDF-15 表达的 Western 印迹分析.
GDF-15 Antibody (H-2) - Western Blotting - Image 356257
GDF-15 抗体 (H-2): sc-515675. LNCaP (A), C6 (B) 和 RIN-m5F (C) 全细胞裂解液及小鼠胎盘组织提取物 (D) 中 GDF-15 表达的 Western 印迹分析.
GDF-15 Antibody (H-2) - Western Blotting - Image 380723
GDF-15 抗体 (H-2): sc-515675. C6 (A) 和 RAW 264. 7 (B) 全细胞裂解液中 GDF-15 表达的 Western 印迹分析.
GDF-15 Antibody (H-2) - Western Blotting - Image 400713
GDF-15 抗体 (H-2) HRP: sc-515675 HRP. LNCaP (A), C6 (B) 和 RIN-m5F (C) 全细胞裂解液中 GDF-15 表达的直接 Western 印迹分析.
GDF-15 抗体 (H-2): sc-515675. LNCaP (A), C6 (B) 和 RIN-m5F (C) 全细胞裂解液及小鼠胎盘组织提取物 (D) 中 GDF-15 表达的 Western 印迹分析.

GDF-15 抗体 (H-2): sc-515675

4.5(2)
寫評論提問

说明书
  • GDF-15抗体(H-2)是小鼠单克隆IgG2a κ, 在12篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于rat物种的的N-terminus附近的氨基酸41-62之间
  • 抗-GDF-15 抗体 (H-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 precursor and mature GDF-15
  • 抗GDF-15抗体（H-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GDF-15 (H-2): sc-515675的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是GDF-15 Antibody (H-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与GDF-15 Antibody (H-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外GDF抗体，包括GDF-11, GDF-15, GDF-16, GDF-5, GDF-6, GDF-7, GDF-8, GDF-9和GDF-9B

GDF-15抗体（H-2）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）在小鼠、大鼠和人类样本中检测生长分化因子15（GDF-15）。抗GDF-15抗体（H-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。GDF-15，也称为PDF、MIC-1、PLAB、NAG-1和PTGF-β，属于转化生长因子β（TGF-β）超家族，在细胞对压力和炎症的反应中起着至关重要的作用。GDF-15在细胞内合成，并以二聚体形式分泌，通过二硫键连接，在上皮细胞和巨噬细胞中表达量最高，同时在成人组织中广泛分布。GDF-15从大脑中的脉络丛分泌到脑脊液中，凸显了其在中枢神经系统功能中的重要性。p53肿瘤抑制蛋白调节GDF-15基因的表达，在小脑颗粒神经元的培养中，GDF-15通过激活Akt通路并抑制ERK信号传导来防止细胞死亡。这种营养因子可促进特定神经元群中的细胞存活和分化。前列腺癌中GDF-15的过度表达可作为潜在的诊断标志物。在怀孕期间，GDF-15可帮助抑制来自母亲的促炎细胞因子，从而提高胎儿的存活率。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GDF-15 抗体 (H-2) 参考文献:

  1. GDF15的代谢效应由孤儿受体GFRAL介导。  |  Emmerson, PJ., et al. 2017. Nat Med. 23: 1215-1219. PMID: 28846098
  2. GDF15是小鼠和人类营养压力的内分泌信号  |  Patel, S., et al. 2019. Cell Metab. 29: 707-718.e8. PMID: 30639358
  3. ARRB1通过促进GDF15的成熟来抑制非酒精性脂肪性肝炎的发展。  |  Zhang, Z., et al. 2020. J Hepatol. 72: 976-989. PMID: 31857195
  4. GDF15 通过激活 AMPK 来介导 PPARβ/δ 的代谢作用。  |  Aguilar-Recarte, D., et al. 2021. Cell Rep. 36: 109501. PMID: 34380027
  5. 一种表达生长因子的巨噬细胞亚群通过GDF-15协调再生炎症。  |  Patsalos, A., et al. 2022. J Exp Med. 219: PMID: 34846534
  6. 生长分化因子15（GDF15）和塞马鲁肽通过截然不同、相互叠加的机制抑制食物摄入和体重增加。  |  Ghidewon, M., et al. 2022. Diabetes Obes Metab. 24: 1010-1020. PMID: 35129264
  7. 内分泌学中的生长分化因子15（GDF-15）。  |  Iglesias, P., et al. 2023. Endocrine. 81: 419-431. PMID: 37129758
  8. 肝脏葡萄糖醛酸 C5-表聚酶通过稳定 GDF15 来对抗肥胖。  |  He, F., et al. 2023. J Hepatol. 79: 605-617. PMID: 37217020
  9. 肾脏疾病中的生长分化因子15（GDF-15）。  |  Delrue, C., et al. 2023. Adv Clin Chem. 114: 1-46. PMID: 37268330
  10. 巨噬细胞作为GDF-15的来源和靶标。  |  Silva-Bermudez, LS., et al. 2024. Int J Mol Sci. 25: PMID: 39000420

订购信息

产品名称产品编号规格价格数量收藏夹

GDF-15 抗体 (H-2)

sc-515675
200 µg/ml
$316.00

GDF-15 (H-2): m-IgG Fc BP-HRP 套装

sc-531464
200 µg Ab; 10 µg BP
$354.00

GDF-15 (H-2): m-IgGκ BP-HRP 套装

sc-525159
200 µg Ab, 40 µg BP
$354.00

GDF-15 (H-2): m-IgG2a BP-HRP 套装

sc-547796
200 µg Ab; 10 µg BP
$354.00

GDF-15 抗体 (H-2) AC

sc-515675 AC
500 µg/ml, 25% agarose
$416.00

GDF-15 抗体 (H-2) HRP

sc-515675 HRP
200 µg/ml
$316.00

GDF-15 抗体 (H-2) FITC

sc-515675 FITC
200 µg/ml
$330.00

GDF-15 抗体 (H-2) PE

sc-515675 PE
200 µg/ml
$343.00

GDF-15 抗体 (H-2) Alexa Fluor® 488

sc-515675 AF488
200 µg/ml
$357.00

GDF-15 抗体 (H-2) Alexa Fluor® 546

sc-515675 AF546
200 µg/ml
$357.00

GDF-15 抗体 (H-2) Alexa Fluor® 594

sc-515675 AF594
200 µg/ml
$357.00

GDF-15 抗体 (H-2) Alexa Fluor® 647

sc-515675 AF647
200 µg/ml
$357.00

GDF-15 抗体 (H-2) Alexa Fluor® 680

sc-515675 AF680
200 µg/ml
$357.00

GDF-15 抗体 (H-2) Alexa Fluor® 790

sc-515675 AF790
200 µg/ml
$357.00

I have a question. I did WB to detect gdf15 in H9C2 cell by use the GDF-15 antibody (H-2): sc-515675. I got 2 band : one above 50 kDa, the other around 35 kDa. is this the right phenomenon?

Asked by: nana
Thank you for your question.It would be helpful if you could call us directly at (+86) 021-60936350 or email our Asia Service department at asia@scbio.cn for more interactive discussions on this and other related issues.
Answered by: Zhanglong
Date published: 2023-12-08

Hi, could you please tell me what the enterotoxin levels are for this product?

Asked by: Giuseppe
Thank you for your question. Enterotoxin levels are not determined for this product.
Answered by: Tech Support Europe
Date published: 2022-08-17

Please tell me the blocking peptide corresponding to this antibody (sc-515675).

Asked by: yuki13
Thank you for your question. We do not offer a blocking peptide for this antibody.
Answered by: Tech Support Europe
Date published: 2021-02-04

I have a question. is western blot data non-reducing conditions or reducing conditions? I did WB to detect gdf15 in LNCaP by non-reducing conditions. I got 2 band : one above 40 kDa, the other around 35 kDa.

Asked by: Col12
Thank you for your question. Our Western blot data with this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
Answered by: Technical Support
Date published: 2023-12-05

I have a rat cell line, can this ab detect GDF15 from rat?

Asked by: M76gmm
Thank you for your question. Yes, this antibody is recommended for detection of GDF15 of mouse, rat and human origin. We have Western blot data on our website in the rat cell line, RIN-m5F.
Answered by: Technical Support
Date published: 2019-01-31

Has anyone any information that this Ab can detect human GDF15. According to the protein alignments in aa 41-62 the Human sequence differs by an additional 7 amino acids. Would be keen to see some experimental data if anyone has it. Thanks

Asked by: Satish Patel
Thank you for your question. Yes, we have human cell data on this antibody in LNCaP cells on the product page: https://www.scbt.com/scbt/product/gdf-15-antibody-h-2?requestFrom=search If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-28

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515675: GDF-15 (H-2) mouse monoclonal antibody will work?

Asked by: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2024-04-16
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Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:500 for overnight at 4oC
Date published: 2024-12-13
Rated 4 out of 5 by from Works well on murine cardiac samples1h blocking in mPBST, GDF15 ab ON at 4°C, secondary ab 1 h at RT; Ab detected 2 bands: one slightly above 40 kDa, the other around 25 kDa
Date published: 2017-11-27
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GDF-15 抗体 (H-2) is rated 4.5 out of 5 by 2.
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