GDF-15抗体 (H-2) 推荐用于 mouse, rat 和 human 来源的precursor and mature GDF-15 WB, IP, IF 和 ELISA检测

GDF-15 抗体 (H-2): sc-515675

GDF-15抗体 (H-2) is rated 4.0 out of 5 by 1.
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说明书

    • GDF-15抗体(H-2)是小鼠单克隆IgG2a κ, 在1篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于rat物种的的N-terminus附近的氨基酸41-62之间
    • 抗-GDF-15 抗体 (H-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 precursor and mature GDF-15
    • 抗-GDF-15 抗体 (H-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取GDF-15 (H-2): sc-515675免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 GDF-15 Antibody (H-2) for WB 的首选应用二抗。这些试剂现在以套装形式提供GDF-15 Antibody (H-2)。 (见下方订购信息).
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Please tell me the blocking peptide corresponding to this antibody (sc-515675).

Asked by: yuki13
Thank you for your question. We do not offer a blocking peptide for this antibody.
Answered by: Tech Support Europe
Date published: 2020-07-29

I have a question. is western blot data non-reducing conditions or reducing conditions? I did WB to detect gdf15 in LNCaP by non-reducing conditions. I got 2 band : one above 40 kDa, the other around 35 kDa.

Asked by: Col12
Thank you for your question. Our Western blot data with this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
Answered by: Technical Support
Date published: 2019-05-25

I have a rat cell line, can this ab detect GDF15 from rat?

Asked by: M76gmm
Thank you for your question. Yes, this antibody is recommended for detection of GDF15 of mouse, rat and human origin. We have Western blot data on our website in the rat cell line, RIN-m5F.
Answered by: Technical Support
Date published: 2019-01-31

Has anyone any information that this Ab can detect human GDF15. According to the protein alignments in aa 41-62 the Human sequence differs by an additional 7 amino acids. Would be keen to see some experimental data if anyone has it. Thanks

Asked by: Satish Patel
Thank you for your question. Yes, we have human cell data on this antibody in LNCaP cells on the product page: https://www.scbt.com/scbt/product/gdf-15-antibody-h-2?requestFrom=search If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-28

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515675: GDF-15 (H-2) mouse monoclonal antibody will work?

Asked by: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 4 out of 5 by from Works well on murine cardiac samples 1h blocking in mPBST, GDF15 ab ON at 4°C, secondary ab 1 h at RT; Ab detected 2 bands: one slightly above 40 kDa, the other around 25 kDa
Date published: 2017-11-27
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