Date published: 2025-9-6

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DDIT3/GADD153/CHOP 抗体 (H-5): sc-166682

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说明书
  • DDIT3/GADD153/CHOP抗体(H-5)是小鼠单克隆IgG3 κ, 在30篇文献中引用,规格为200 µg/ml
  • 与氨基酸序列C-terminus(h)对应的多肽序列(GADD 153的human起源)
  • 抗-DDIT3/GADD153/CHOP 抗体 (H-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 GADD 153; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗DDIT3/GADD153/CHOP抗体(H-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DDIT3/GADD153/CHOP (H-5): sc-166682免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP 是DDIT3/GADD153/CHOP 抗体 (H-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与DDIT3/GADD153/CHOP 抗体 (H-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

DDIT3/CHOP/GADD153抗体(H-5)是一种IgG3κ小鼠单克隆GADD 153抗体(也称为GADD 153抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的GADD 153。DDIT3/CHOP/GADD153抗体(H-5)可以以非偶联的抗GADD 153抗体形式提供,也可以以多种偶联形式的抗GADD 153抗体形式提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。GADD 153被描述为一种生长阻滞和DNA损伤诱导型基因,编码一种与C/EBP相关的核蛋白。该蛋白也被指定为C/EBP同源蛋白(CHOP-10)。GADD 153的表达受到各种细胞应激的诱导,包括营养缺乏和代谢紊乱。GADD 153的功能是阻止细胞在G1到S期的细胞周期进程,并通过与其他C/EBP蛋白二聚化来指导GADD 153二聚体远离"经典"C/EBP结合位点,而是识别出独特的"非经典"位点。因此,GADD 153在某些终末分化细胞中充当负调节剂,类似于Id对Myo D和Myo D相关蛋白活性的调节作用,这些蛋白参与肌肉细胞的发育。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DDIT3/GADD153/CHOP 抗体 (H-5) 参考文献:

  1. 调控的翻译起始控制哺乳动物细胞中应激诱导的基因表达。  |  Harding, HP., et al. 2000. Mol Cell. 6: 1099-108. PMID: 11106749
  2. 了解间质癌:以脂肪肉瘤相关的 FUS-DDIT3 融合基因为模型。  |  Pérez-Mancera, PA. and Sánchez-García, I. 2005. Semin Cancer Biol. 15: 206-14. PMID: 15826835
  3. 黄酮素B通过内质网应激诱导的细胞自噬来抑制胶质瘤的生长。  |  Wang, J., et al. 2018. Autophagy. 14: 2007-2022. PMID: 30025493
  4. DDIT3(CHOP)可导致DBA/2J小鼠视网膜神经节细胞体细胞丢失,但不会导致轴突变性。  |  Marola, OJ., et al. 2019. Cell Death Discov. 5: 140. PMID: 31632741
  5. DDIT3 通过 DDIT3-SQSTM1-STING 通路拮抗先天性免疫反应,促进牛阿尔法疱疹病毒 1 的复制。  |  Wang, S., et al. 2022. Virulence. 13: 514-529. PMID: 35259065
  6. FUS::DDIT3融合肿瘤蛋白可抑制肌样脂肪肉瘤中BAF复合物的靶向和活性。  |  Zullow, HJ., et al. 2022. Mol Cell. 82: 1737-1750.e8. PMID: 35390276
  7. 一种新型DDIT3激活剂脱氢吴茱萸碱可有效抑制胰腺癌的肿瘤生长和肿瘤细胞干细胞特性。  |  Zhu, SL., et al. 2024. Phytomedicine. 128: 155377. PMID: 38503154
  8. DDIT3与乳腺癌预后和免疫微环境有关:生物信息学和免疫组织化学综合分析。  |  Yu, X., et al. 2024. J Cancer. 15: 3873-3889. PMID: 38911383
  9. 转录因子DDIT3是胰腺癌的潜在驱动因子。  |  Ju, G., et al. 2024. Cell Mol Biol (Noisy-le-grand). 70: 148-154. PMID: 39097882
  10. DDIT3通过上调间充质干细胞中的DKK1,减弱Wnt/β-catenin信号传导,从而将BMP9的成骨潜能转化为脂肪生成潜能。  |  Luo, HH., et al. 2024. Aging (Albany NY). 16: 12543-12558. PMID: 39331002

订购信息

产品名称产品编号规格价格数量收藏夹

DDIT3/GADD153/CHOP 抗体 (H-5)

sc-166682
200 µg/ml
$316.00

DDIT3/CHOP/GADD153 (H-5): m-IgG3 BP-HRP 套装

sc-550557
200 µg Ab; 40 µg BP
$354.00

DDIT3/CHOP/GADD153 抗体 (H-5) AC

sc-166682 AC
500 µg/ml, 25% agarose
$416.00

DDIT3/CHOP/GADD153 抗体 (H-5) HRP

sc-166682 HRP
200 µg/ml
$316.00

DDIT3/CHOP/GADD153 抗体 (H-5) FITC

sc-166682 FITC
200 µg/ml
$330.00

DDIT3/CHOP/GADD153 抗体 (H-5) PE

sc-166682 PE
200 µg/ml
$343.00

DDIT3/CHOP/GADD153 抗体 (H-5) Alexa Fluor® 488

sc-166682 AF488
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (H-5) Alexa Fluor® 546

sc-166682 AF546
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (H-5) Alexa Fluor® 594

sc-166682 AF594
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (H-5) Alexa Fluor® 647

sc-166682 AF647
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (H-5) Alexa Fluor® 680

sc-166682 AF680
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (H-5) Alexa Fluor® 790

sc-166682 AF790
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 (H-5) 中和勝肽

sc-166682 P
100 µg/0.5 ml
$68.00

Hi, for IHC use, what is the suggested pH for antigen retrieval pH? Citrate buffer pH 6.0 or TE buffer pH 9.0? Thanks!

Asked by: JessyQ
Thank you for your question. We would suggest using sodium citrate buffer pH 6 for antigen retrieval.
Answered by: Tech Service
Date published: 2020-05-17

What is the protocol for the negative control blocking peptide sc-166682 P?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use by preincubating with the primary antibody to block the specific reactivity. The details of the protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 5
Date published: 2017-01-19

As "GADD153(H-5) :sc-166682" antibody is labeled as "IgG2b (kappa light chain)", could I use anti-mouse IgG secondary antibody? Or I need to use special secondary antibody?

Asked by: huihui
Thank you for your question. Yes, you can use any anti-mouse IgG secondary antibody for detection of our primary antibody, GADD153 (H-5): sc-166682. This primary antibody has an isotype of IgG2b (kappa light chain). An isotype-specific secondary antibody may be used, but it is not required.
Answered by: Technical Support
Date published: 2016-10-07
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from gongnengchuse看到文献里用的是这个产品 所以来订购的 之前有买过其他品牌的这个蛋白的抗体 然而不好用 做了很久 用这个试试 好用会推荐给周围人的
Date published: 2017-08-15
Rated 5 out of 5 by from Superb productNice western blot data; Also great product for IHC staining
Date published: 2017-02-02
Rated 5 out of 5 by from Works Great!This antibody works great in western blot analysis of full length mouse recombinant GADD 153 fusion protein.
Date published: 2017-01-11
Rated 5 out of 5 by from Clean band for WB in mouse GADD 153 transfected(B)Clean band for WB in mouse GADD 153 transfected(B) 293T whole cell lysates. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Good signal strength for western blot analysisGood signal strength for western blot analysis of full length mouse recombinant GADD 153 fusion protein. -SCBT QC
Date published: 2013-05-04
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DDIT3/GADD153/CHOP 抗体 (H-5) is rated 5.0 out of 5 by 5.
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