Date published: 2026-10-22
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GAD-65/67 Antibody (C-9) - Western Blotting - Image 312782
GAD-65/67 抗体 (C-9): sc-365180. PANC-1 (A), A431 (B) 和 U-251-MG (C) 全细胞裂解液中 GAD-65/67 表达的 Western 印迹分析.
GAD-65/67 抗体 (C-9): sc-365180. PANC-1 (A), A431 (B) 和 U-251-MG (C) 全细胞裂解液中 GAD-65/67 表达的 Western 印迹分析.

GAD-65/67 抗体 (C-9): sc-365180

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说明书
  • GAD-65/67 抗体 C-9 是小鼠单克隆 IgG1 κ，GAD-65/67抗体, 在31篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的GAD-67的C-terminus的氨基酸569-594之间
  • GAD-65/67 抗体 (C-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GAD-65 and GAD-67; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗GAD-65/67抗体（C-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是GAD-65/67 Antibody (C-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与GAD-65/67 Antibody (C-9) 打包提供（请参阅下面的订购信息）。
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関連項目

附加GAD抗体，包括GAD-65和GAD-67

GAD-65/67抗体（C-9）是一种IgG1 κ型小鼠单克隆GAD-65/67抗体（也称为GAD65/67抗体或GAD1/2抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组化（IHC(P)）和酶联免疫吸附测定（ELISA）等方法检测来自小鼠、大鼠和人类的GAD-65/67蛋白。GAD-65/67抗体（C-9）既有非偶联形式的抗GAD-65/67抗体，也有多种偶联形式的抗GAD-65/67抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。大脑中存在两种形式的谷氨酸脱羧酶（GADs）：GAD-65（也称为GAD2）和GAD-67（也称为GAD1、GAD或SCP）。GAD-65和GAD-67是II型脱羧酶家族蛋白的成员，负责催化L-谷氨酸产生GABA（γ-氨基丁酸）的限速步骤。尽管两种GADs都存在于大脑中，但GAD-65定位于神经末梢的突触囊泡膜上，而GAD-67则分布于整个细胞中。GAD-67负责GABA合成的基础水平。在神经传递中对GABA需求增加的情况下，GAD-65会短暂激活以协助GABA的产生。GAD-65的缺失是有害的，可能会损害GABA的神经传递，但GAD-67的缺失是致命的。由于选择性剪接，GAD-67存在两种异构体，即主要的GAD-67形式和次要的GAD-25形式。GAD-25不在大脑中表达，但可以在各种内分泌组织中找到。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAD-65/67 抗体 (C-9) 参考文献:

  1. 神经元活动和神经营养因子调控大鼠视皮层器官型培养物中 GAD-65/67 mRNA 和蛋白的表达  |  Patz, S., et al. 2003. Eur J Neurosci. 18: 1-12. PMID: 12859332
  2. 大鼠初级听觉皮层中与年龄相关的GABA合成酶谷氨酸脱羧酶的丢失。  |  Ling, LL., et al. 2005. Neuroscience. 132: 1103-13. PMID: 15857714
  3. 抑郁症患者下丘脑室旁核 GAD(65/67) 免疫活性降低：一项尸检研究。  |  Gao, SF., et al. 2013. J Affect Disord. 149: 422-5. PMID: 23312397
  4. 人类致痫性局灶性皮质发育不良中不同的微球蛋白和GABA表达。  |  Medici, V., et al. 2016. Epilepsia. 57: 1109-19. PMID: 27173597
  5. 多巴胺D4受体的药理激活可调节吗啡诱导的基底神经节GAD65/67和GABAB受体表达的变化。  |  Negrete-Díaz, JV., et al. 2019. Neuropharmacology. 152: 22-29. PMID: 30682345
  6. 耳蜗核中Nav1.6的异常表达与水杨酸盐诱导的大鼠耳鸣有关。  |  Yin, M., et al. 2020. Biochem Biophys Res Commun. 526: 786-792. PMID: 32268959
  7. 单细胞多重定量PCR证实，腹内侧下丘脑核GABA能神经元存在性别差异的谷氨酸脱羧酶、雌激素受体和5'-AMP激活的蛋白激酶α亚基mRNA表达。  |  Ali, MH., et al. 2022. J Chem Neuroanat. 124: 102132. PMID: 35772680
  8. 葡萄籽提取物可抑制降钙素基因相关肽的分泌，并上调原发性三叉神经节培养物中 GAD 65/67 和 GABAB 受体的表达。  |  Antonopoulos, SR. and Durham, PL. 2022. IBRO Neurosci Rep. 13: 187-197. PMID: 36093283
  9. 菊花及其生物活性物质通过激活 Cl- 通道提高啮齿动物模型的睡眠质量  |  Kim, M., et al. 2023. Nutrients. 15: PMID: 36986039
  10. 在亨廷顿病大鼠模型中，重新编程的人外侧神经节隆起前体可生成纹状体神经元并恢复运动功能。  |  McCaughey-Chapman, A., et al. 2024. Stem Cell Res Ther. 15: 448. PMID: 39578834

订购信息

产品名称产品编号规格价格数量收藏夹

GAD-65/67 抗体 (C-9)

sc-365180
200 µg/ml
$322.00

GAD-65/67 (C-9): m-IgG Fc BP-HRP 套装

sc-529321
200 µg Ab; 10 µg BP
$361.00

GAD-65/67 (C-9): m-IgGκ BP-HRP 套装

sc-522154
200 µg Ab, 40 µg BP
$361.00

GAD-65/67 (C-9): m-IgG1 BP-HRP 套装

sc-543455
200 µg Ab; 20 µg BP
$361.00

GAD-65/67 抗体 (C-9) AC

sc-365180 AC
500 µg/ml, 25% agarose
$424.00

GAD-65/67 抗体 (C-9) HRP

sc-365180 HRP
200 µg/ml
$322.00

GAD-65/67 抗体 (C-9) FITC

sc-365180 FITC
200 µg/ml
$336.00

GAD-65/67 抗体 (C-9) PE

sc-365180 PE
200 µg/ml
$349.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 488

sc-365180 AF488
200 µg/ml
$364.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 546

sc-365180 AF546
200 µg/ml
$364.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 594

sc-365180 AF594
200 µg/ml
$364.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 647

sc-365180 AF647
200 µg/ml
$364.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 680

sc-365180 AF680
200 µg/ml
$364.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 790

sc-365180 AF790
200 µg/ml
$364.00

GAD-65/67 (C-9) 中和勝肽

sc-365180 P
100 µg/0.5 ml
$69.00

Hi, I hope you are in good health and doing well. I have GAD65/67 antibodies for Western blot but it's not working. I saw Santacruz also has a GAD65/67 antibody. Is it possible to get a free sample? Best, Shekhar

Asked by: Anonymous
Thank you for your question. Please get in touch with our technical service team for assistance.
Answered by: Tech Support Europe
Date published: 2024-03-28

can you ship to Egypt

Asked by: Salah Elsaid
We do! Please contact our Germany based team or the local Egypt distributor.
Answered by: Tech Sales
Date published: 2021-06-25

What is the recommended fixation method for IF with GAD-65/67 (C-9): sc-365180 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2017-02-03
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Rated 4 out of 5 by Conte from Good antibodyTested the antibody in immunofluorescent staining on human iPSC-derived neurons at 1:50 dilution. The antibody stains the cell bodies of a fraction of neurons as expected in this kind of culture that should have around 10-15% GABAergic neurons. Image shows sc-365180 Mouse anti-GAD65/67 (green), rabbit anti-Synapsin 1 (red), chicken anti-TubB3 (blue), nuclei are counterstained with DAPI (gray).
Date published: 2024-06-13
Rated 5 out of 5 by mfzappa from Western blot of glutamic acid decarboxylasesGood Western blot data of glutamic acid decarboxylases (GAD) expression, in their isoforms GAD65 and 67, in rat hippocampal brain tissue. Specific band at 1:200 dilution.
Date published: 2019-08-13
Rated 5 out of 5 by HAO Q from Very strong antibody for immunostainingI asked a free sample and tested it in my immunofluorescence staining on the midbrain of mouse. With 1:100 dilution, the signal is very strong and clear.
Date published: 2017-11-10
Rated 5 out of 5 by DoctorStrange from Excellent immunostaining for IHC-P of mouse brainThis antibody worked very well in immunofluorescence of formalin-fixed paraffin embedded mouse brain tissues, antigen retrieval used Pascal Citrate pH6, concentration 1:100.
Date published: 2017-08-15
Rated 5 out of 5 by RenXY from in the Western BlotReliable antibody for Western blot, this antibody gives a strong band in rat brain tissue
Date published: 2017-06-01
Rated 5 out of 5 by Sweetums from Good antibodyStrong, clean bands observed in Western blot for GAD-65/67 detection.
Date published: 2017-02-07
Rated 3 out of 5 by FL from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-04-03
Rated 4 out of 5 by MS from Nice band detected in WB using HUVNice band detected in WB using HUV-EC-C whole cell lysate. -SCBT QC
Date published: 2015-03-24
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GAD-65/67抗体 (C-9) is rated 4.4 out of 5 by 9.
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