Date published: 2025-9-6

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GAD-65/67 抗体 (C-9): sc-365180

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说明书
  • GAD-65/67 抗体 C-9 是小鼠单克隆 IgG1 κ,GAD-65/67抗体, 在31篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的GAD-67的C-terminus的氨基酸569-594之间
  • GAD-65/67 抗体 (C-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 GAD-65 and GAD-67; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗GAD-65/67抗体(C-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GAD-65/67 (C-9): sc-365180免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 GAD-65/67 抗体 (C-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GAD-65/67 抗体 (C-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

GAD-65/67抗体(C-9)是一种IgG1 κ型小鼠单克隆GAD-65/67抗体(也称为GAD65/67抗体或GAD1/2抗体),可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组化(IHC(P))和酶联免疫吸附测定(ELISA)等方法检测来自小鼠、大鼠和人类的GAD-65/67蛋白。GAD-65/67抗体(C-9)既有非偶联形式的抗GAD-65/67抗体,也有多种偶联形式的抗GAD-65/67抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。大脑中存在两种形式的谷氨酸脱羧酶(GADs):GAD-65(也称为GAD2)和GAD-67(也称为GAD1、GAD或SCP)。GAD-65和GAD-67是II型脱羧酶家族蛋白的成员,负责催化L-谷氨酸产生GABA(γ-氨基丁酸)的限速步骤。尽管两种GADs都存在于大脑中,但GAD-65定位于神经末梢的突触囊泡膜上,而GAD-67则分布于整个细胞中。GAD-67负责GABA合成的基础水平。在神经传递中对GABA需求增加的情况下,GAD-65会短暂激活以协助GABA的产生。GAD-65的缺失是有害的,可能会损害GABA的神经传递,但GAD-67的缺失是致命的。由于选择性剪接,GAD-67存在两种异构体,即主要的GAD-67形式和次要的GAD-25形式。GAD-25不在大脑中表达,但可以在各种内分泌组织中找到。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GAD-65/67 抗体 (C-9)

sc-365180
200 µg/ml
$316.00

GAD-65/67 (C-9): m-IgG Fc BP-HRP 套装

sc-529321
200 µg Ab; 10 µg BP
$354.00

GAD-65/67 (C-9): m-IgGκ BP-HRP 套装

sc-522154
200 µg Ab, 40 µg BP
$354.00

GAD-65/67 (C-9): m-IgG1 BP-HRP 套装

sc-543455
200 µg Ab; 20 µg BP
$354.00

GAD-65/67 抗体 (C-9) AC

sc-365180 AC
500 µg/ml, 25% agarose
$416.00

GAD-65/67 抗体 (C-9) HRP

sc-365180 HRP
200 µg/ml
$316.00

GAD-65/67 抗体 (C-9) FITC

sc-365180 FITC
200 µg/ml
$330.00

GAD-65/67 抗体 (C-9) PE

sc-365180 PE
200 µg/ml
$343.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 488

sc-365180 AF488
200 µg/ml
$357.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 546

sc-365180 AF546
200 µg/ml
$357.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 594

sc-365180 AF594
200 µg/ml
$357.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 647

sc-365180 AF647
200 µg/ml
$357.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 680

sc-365180 AF680
200 µg/ml
$357.00

GAD-65/67 抗体 (C-9) Alexa Fluor® 790

sc-365180 AF790
200 µg/ml
$357.00

GAD-65/67 (C-9) 中和勝肽

sc-365180 P
100 µg/0.5 ml
$68.00

Hi, I hope you are in good health and doing well. I have GAD65/67 antibodies for Western blot but it's not working. I saw Santacruz also has a GAD65/67 antibody. Is it possible to get a free sample? Best, Shekhar

Asked by: Anonymous
Thank you for your question. Please get in touch with our technical service team for assistance.
Answered by: Tech Support Europe
Date published: 2024-03-28

can you ship to Egypt

Asked by: Salah Elsaid
We do! Please contact our Germany based team or the local Egypt distributor.
Answered by: Tech Sales
Date published: 2021-06-25

What is the recommended fixation method for IF with GAD-65/67 (C-9): sc-365180 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2017-02-03
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Rated 4 out of 5 by from Good antibodyTested the antibody in immunofluorescent staining on human iPSC-derived neurons at 1:50 dilution. The antibody stains the cell bodies of a fraction of neurons as expected in this kind of culture that should have around 10-15% GABAergic neurons. Image shows sc-365180 Mouse anti-GAD65/67 (green), rabbit anti-Synapsin 1 (red), chicken anti-TubB3 (blue), nuclei are counterstained with DAPI (gray).
Date published: 2024-06-13
Rated 5 out of 5 by from Western blot of glutamic acid decarboxylasesGood Western blot data of glutamic acid decarboxylases (GAD) expression, in their isoforms GAD65 and 67, in rat hippocampal brain tissue. Specific band at 1:200 dilution.
Date published: 2019-08-13
Rated 5 out of 5 by from Very strong antibody for immunostainingI asked a free sample and tested it in my immunofluorescence staining on the midbrain of mouse. With 1:100 dilution, the signal is very strong and clear.
Date published: 2017-11-10
Rated 5 out of 5 by from Excellent immunostaining for IHC-P of mouse brainThis antibody worked very well in immunofluorescence of formalin-fixed paraffin embedded mouse brain tissues, antigen retrieval used Pascal Citrate pH6, concentration 1:100.
Date published: 2017-08-15
Rated 5 out of 5 by from in the Western BlotReliable antibody for Western blot, this antibody gives a strong band in rat brain tissue
Date published: 2017-06-01
Rated 5 out of 5 by from Good antibodyStrong, clean bands observed in Western blot for GAD-65/67 detection.
Date published: 2017-02-07
Rated 3 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-04-03
Rated 4 out of 5 by from Nice band detected in WB using HUVNice band detected in WB using HUV-EC-C whole cell lysate. -SCBT QC
Date published: 2015-03-24
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GAD-65/67抗体 (C-9) is rated 4.4 out of 5 by 9.
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