Date published: 2025-9-7

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Flotillin-2 抗体 (B-6): sc-28320

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说明书
  • Flotillin-2 抗体 B-6 是小鼠单克隆 IgG1 κ,Flotillin-2抗体, 在59篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Flotillin-2的氨基酸151-240
  • Flotillin-2 抗体 (B-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Flotillin-2
  • 抗Flotillin-2抗体(B-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Flotillin-2 (B-6): sc-28320免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Flotillin-2 抗体 (B-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Flotillin-2 抗体 (B-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Flotillin-2抗体(B-6)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Flotillin-2蛋白。抗Flotillin-2抗体(B-6)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Flotillin-2,又称表皮表面抗原,在脂筏的形成和维持中发挥着关键作用。脂筏是富含鞘脂和胆固醇的膜微区,对于信号转导、膜运输和分子分拣等众多细胞过程至关重要。这些脂筏充当信号复合物组装的平台,促进细胞内和细胞间的有效通信。Flotillin-2在各种组织中普遍表达,Flotillin-2与其他蛋白质(如CAP和Cbl)的相互作用对于将信号复合物定位到细胞膜特定脂筏亚域至关重要。这种定位对于快速激活信号通路(如MAP激酶级联反应)以响应雌激素等外部刺激尤为重要。此外,浮萍蛋白的表达水平是独立调节的,并且与阿尔茨海默氏症的病理进展相关,这凸显了浮萍蛋白在正常细胞功能和疾病状态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Flotillin-2 抗体 (B-6) 参考文献:

  1. 氟替林素/空穴素在细胞和组织中不同程度地表达,并在体内与空穴素形成异构体复合物。新型氟替林素-1单克隆抗体探针的特征和表位图谱。  |  Volonte, D., et al. 1999. J Biol Chem. 274: 12702-9. PMID: 10212252
  2. Flotillin 2 与表皮表面抗原(ESA)不同,与丝状体的形成有关。  |  Hazarika, P., et al. 1999. J Cell Biochem. 75: 147-59. PMID: 10462713
  3. 人脑中氟替林素的定域及其随阿尔茨海默病病理进展的积累。  |  Kokubo, H., et al. 2000. Neurosci Lett. 290: 93-6. PMID: 10936685
  4. 雌激素在大脑中作用的新部位和机制。  |  Toran-Allerand, CD. 2000. Novartis Found Symp. 230: 56-69; discussion 69-73. PMID: 10965502
  5. CAP 定义了胰岛素刺激葡萄糖转运所需的第二个信号通路。  |  Baumann, CA., et al. 2000. Nature. 407: 202-7. PMID: 11001060
  6. Stomatin, flotillin-1 和 flotillin-2 是红细胞脂质筏的主要整合蛋白。  |  Salzer, U. and Prohaska, R. 2001. Blood. 97: 1141-3. PMID: 11159550
  7. 富集在成熟吞噬体上的 Flotillin-1 脂质筏结构域。  |  Dermine, JF., et al. 2001. J Biol Chem. 276: 18507-12. PMID: 11279173
  8. Flotillin-2缺乏导致小鼠乳腺癌模型肺部转移减少。  |  Berger, T., et al. 2013. Oncogene. 32: 4989-94. PMID: 23146906
  9. Flotillins(flotillin-1和flotillin-2)在人类癌症中的预后价值:荟萃分析。  |  Deng, Y., et al. 2018. Clin Chim Acta. 481: 90-98. PMID: 29499201
  10. Flotillin-2 预测肝内胆管癌的不良预后并促进肿瘤侵袭。  |  Xu, Z., et al. 2020. Oncol Lett. 19: 2243-2250. PMID: 32194722
  11. Flotillin-2调节表皮生长因子受体的活化, 通过Cbl介导的泛素化降解以及癌症生长。  |  Wisniewski, DJ., et al. 2023. J Biol Chem. 299: 102766. PMID: 36470425
  12. 新型表皮细胞表面抗原(ESA)的克隆和特征描述。  |  Schroeder, WT., et al. 1994. J Biol Chem. 269: 19983-91. PMID: 8051082

订购信息

产品名称产品编号规格价格数量收藏夹

Flotillin-2 抗体 (B-6)

sc-28320
200 µg/ml
$316.00

Flotillin-2 (B-6): m-IgG Fc BP-HRP 套装

sc-528421
200 µg Ab; 10 µg BP
$354.00

Flotillin-2 (B-6): m-IgGκ BP-HRP 套装

sc-520795
200 µg Ab, 40 µg BP
$354.00

Flotillin-2 (B-6): m-IgG1 BP-HRP 套装

sc-542923
200 µg Ab; 20 µg BP
$354.00

Flotillin-2 抗体 (B-6) AC

sc-28320 AC
500 µg/ml, 25% agarose
$416.00

Flotillin-2 抗体 (B-6) HRP

sc-28320 HRP
200 µg/ml
$316.00

Flotillin-2 抗体 (B-6) FITC

sc-28320 FITC
200 µg/ml
$330.00

Flotillin-2 抗体 (B-6) PE

sc-28320 PE
200 µg/ml
$343.00

Flotillin-2 抗体 (B-6) Alexa Fluor® 488

sc-28320 AF488
200 µg/ml
$357.00

Flotillin-2 抗体 (B-6) Alexa Fluor® 546

sc-28320 AF546
200 µg/ml
$357.00

Flotillin-2 抗体 (B-6) Alexa Fluor® 594

sc-28320 AF594
200 µg/ml
$357.00

Flotillin-2 抗体 (B-6) Alexa Fluor® 647

sc-28320 AF647
200 µg/ml
$357.00

Flotillin-2 抗体 (B-6) Alexa Fluor® 680

sc-28320 AF680
200 µg/ml
$357.00

Flotillin-2 抗体 (B-6) Alexa Fluor® 790

sc-28320 AF790
200 µg/ml
$357.00

お世話になります。Flotillin-2 (B-6): sc-28320のサンプルを頂くことは可能でしょうか。

Asked by: Ayumu
Thank you very much for your question. Please ask your Japanese dealer to apply for sample products. Website: www.cosmobio.co.jp , www.iwai-chem.co.jp or www.nacalai.co.jp/ss/Contact/ 質問頂き、誠にありがとうございます。サンプル品の申請は日本のディーラーにお尋ねお願いします。ウェブサイト: www.cosmobio.co.jp , www.iwai-chem.co.jp 又はwww.nacalai.co.jp/ss/Contact/
Answered by: Jiajun Z
Date published: 2024-11-01

Is it possible to use this mouse monoclonal Flotillin-2 Antibody (B-6): sc-28320 for IHC or IF on mouse tissue samples?

Asked by: Anna99
Yes. This can be done using a directly conjugated primary antibody. This Flotillin-2 Antibody is available conjugated to HRP (sc-28320 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-28320 PE), fluorescein (sc-28320 FITC), Alexa Fluor® 488 (sc-28320 AF488) or Alexa Fluor® 647 (sc-28320 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 13
Date published: 2017-01-16
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Rated 5 out of 5 by from Perfect!I have used this antibody for no transfected HEK293T cell lysate and it worked very well. I saw very sharp bands without any background. conditions: dilution 1:500 in 5%milk incubation for 2h at R.T
Date published: 2021-01-29
Rated 5 out of 5 by from Very good for Western BlotI checked this antibody on Western Blot and it worked very well - used it to check if my siRNAs are working, so I could see that antibody is specific.
Date published: 2018-02-16
Rated 5 out of 5 by from good producti bought this antibody a month a ago and did experiment in hela cell line did a good data
Date published: 2017-09-12
Rated 5 out of 5 by from Nice and clean bandWe used at 1:2000 in Jurkat cells, and got a nice and specific band at 43kd.
Date published: 2017-08-18
Rated 5 out of 5 by from Our most popular Flotillin antibodyFlotillin-2 (B-6) gives a very nice clean band for Western blot (WB) at 42 kDa.
Date published: 2016-12-21
Rated 5 out of 5 by from Good specificity in WB in human HEK293 cellsGood specificity in WB in human HEK293 cells. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Good publishable data in IF in human andGood publishable data in IF in human and mouse HEK 293, NIH 3T3, PC12. -SCBT Publication Review
Date published: 2015-05-02
Rated 5 out of 5 by from Good staining in iHC in monkey brain samplesGood staining in iHC in monkey brain samples. -SCBT Publication Review
Date published: 2015-04-19
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Flotillin-2 抗体 (B-6) is rated 5.0 out of 5 by 14.
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